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Title: Purification, crystallization and preliminary X-ray diffraction studies of d-tagatose 3-epimerase from Pseudomonas cichorii

Abstract

Recombinant d-tagatose 3-epimerase from P. cichorii was purified and crystallized. Diffraction data were collected to 2.5 Å resolution. d-Tagatose 3-epimerase (D-TE) from Pseudomonas cichorii catalyzes the epimerization of various ketohexoses at the C3 position. The epimerization of d-psicose has not been reported with epimerases other than P. cichorii D-TE and d-psicose 3-epimerase from Agrobacterium tumefaciens. Recombinant P. cichorii D-TE has been purified and crystallized. Crystals of P. cichorii D-TE were obtained by the sitting-drop method at room temperature. The crystal belongs to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 76.80, b = 94.92, c = 91.73 Å, β = 102.82°. Diffraction data were collected to 2.5 Å resolution. The asymmetric unit is expected to contain four molecules.

Authors:
;  [1]; ; ;  [2];  [1]
  1. Molecular Structure Research Group, Information Technology Center and Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan)
  2. Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center, Kagawa University, Miki-cho, Kagawa 761-0795 (Japan)
Publication Date:
OSTI Identifier:
22360261
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 2; Other Information: PMCID: PMC2330126; PMID: 17277456; PUBLISHER-ID: gx5105; OAI: oai:pubmedcentral.nih.gov:2330126; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; MOLECULES; RESOLUTION; SPACE GROUPS; X-RAY DIFFRACTION

Citation Formats

Yoshida, Hiromi, Yamada, Mitsugu, Nishitani, Takeyori, Takada, Goro, Izumori, Ken, and Kamitori, Shigehiro, E-mail: kamitori@med.kagawa-u.ac.jp. Purification, crystallization and preliminary X-ray diffraction studies of d-tagatose 3-epimerase from Pseudomonas cichorii. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107001169.
Yoshida, Hiromi, Yamada, Mitsugu, Nishitani, Takeyori, Takada, Goro, Izumori, Ken, & Kamitori, Shigehiro, E-mail: kamitori@med.kagawa-u.ac.jp. Purification, crystallization and preliminary X-ray diffraction studies of d-tagatose 3-epimerase from Pseudomonas cichorii. United Kingdom. doi:10.1107/S1744309107001169.
Yoshida, Hiromi, Yamada, Mitsugu, Nishitani, Takeyori, Takada, Goro, Izumori, Ken, and Kamitori, Shigehiro, E-mail: kamitori@med.kagawa-u.ac.jp. Thu . "Purification, crystallization and preliminary X-ray diffraction studies of d-tagatose 3-epimerase from Pseudomonas cichorii". United Kingdom. doi:10.1107/S1744309107001169.
@article{osti_22360261,
title = {Purification, crystallization and preliminary X-ray diffraction studies of d-tagatose 3-epimerase from Pseudomonas cichorii},
author = {Yoshida, Hiromi and Yamada, Mitsugu and Nishitani, Takeyori and Takada, Goro and Izumori, Ken and Kamitori, Shigehiro, E-mail: kamitori@med.kagawa-u.ac.jp},
abstractNote = {Recombinant d-tagatose 3-epimerase from P. cichorii was purified and crystallized. Diffraction data were collected to 2.5 Å resolution. d-Tagatose 3-epimerase (D-TE) from Pseudomonas cichorii catalyzes the epimerization of various ketohexoses at the C3 position. The epimerization of d-psicose has not been reported with epimerases other than P. cichorii D-TE and d-psicose 3-epimerase from Agrobacterium tumefaciens. Recombinant P. cichorii D-TE has been purified and crystallized. Crystals of P. cichorii D-TE were obtained by the sitting-drop method at room temperature. The crystal belongs to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 76.80, b = 94.92, c = 91.73 Å, β = 102.82°. Diffraction data were collected to 2.5 Å resolution. The asymmetric unit is expected to contain four molecules.},
doi = {10.1107/S1744309107001169},
journal = {Acta Crystallographica. Section F},
number = Pt 2,
volume = 63,
place = {United Kingdom},
year = {Thu Feb 01 00:00:00 EST 2007},
month = {Thu Feb 01 00:00:00 EST 2007}
}