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Title: Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae

Abstract

Heat-shock protein Hsp33 from S. cerevisiae was crystallized and diffraction data were collected to 2.7 Å resolution. The heat-shock protein Hsp33 from the yeast Saccharomyces cerevisiae has been overexpressed, purified and crystallized. A crystal was obtained using the hanging-drop vapour-diffusion method and a data set was collected to 2.7 Å resolution. The crystal belongs to space group P4{sub 3}2{sub 1}2, with unit-cell parameters a = b = 96.43, c = 132.22 Å, α = β = γ = 90°. The asymmetric unit is assumed to contain two subunits of Hsp33, with a V{sub M} value of 2.96 Å{sup 3} Da{sup −1} and a solvent content of 58.41%.

Authors:
; ; ;  [1]
  1. Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027 (China)
Publication Date:
OSTI Identifier:
22360260
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 2; Other Information: PMCID: PMC2330124; PMID: 17277453; PUBLISHER-ID: fw5121; OAI: oai:pubmedcentral.nih.gov:2330124; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; DIFFUSION; RESOLUTION; SOLVENTS; SPACE GROUPS

Citation Formats

Liu, Wei, Zhou, Ye-Yun, Teng, Mai-Kun, and Zhou, Cong-Zhao, E-mail: zcz@ustc.edu.cn. Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107000681.
Liu, Wei, Zhou, Ye-Yun, Teng, Mai-Kun, & Zhou, Cong-Zhao, E-mail: zcz@ustc.edu.cn. Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae. United Kingdom. doi:10.1107/S1744309107000681.
Liu, Wei, Zhou, Ye-Yun, Teng, Mai-Kun, and Zhou, Cong-Zhao, E-mail: zcz@ustc.edu.cn. Thu . "Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae". United Kingdom. doi:10.1107/S1744309107000681.
@article{osti_22360260,
title = {Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae},
author = {Liu, Wei and Zhou, Ye-Yun and Teng, Mai-Kun and Zhou, Cong-Zhao, E-mail: zcz@ustc.edu.cn},
abstractNote = {Heat-shock protein Hsp33 from S. cerevisiae was crystallized and diffraction data were collected to 2.7 Å resolution. The heat-shock protein Hsp33 from the yeast Saccharomyces cerevisiae has been overexpressed, purified and crystallized. A crystal was obtained using the hanging-drop vapour-diffusion method and a data set was collected to 2.7 Å resolution. The crystal belongs to space group P4{sub 3}2{sub 1}2, with unit-cell parameters a = b = 96.43, c = 132.22 Å, α = β = γ = 90°. The asymmetric unit is assumed to contain two subunits of Hsp33, with a V{sub M} value of 2.96 Å{sup 3} Da{sup −1} and a solvent content of 58.41%.},
doi = {10.1107/S1744309107000681},
journal = {Acta Crystallographica. Section F},
number = Pt 2,
volume = 63,
place = {United Kingdom},
year = {Thu Feb 01 00:00:00 EST 2007},
month = {Thu Feb 01 00:00:00 EST 2007}
}