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Title: Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus

Abstract

The molybdenum-cofactor biosynthesis protein C from T. thermophilus has been crystallized in two different space groups, P2{sub 1} and R32; the crystals diffracted to 1.9 and 1.75 Å resolution, respectively. The Gram-negative aerobic eubacterium Thermus thermophilus is an extremely important thermophilic microorganism that was originally isolated from a thermal vent environment in Japan. The molybdenum cofactor in this organism is considered to be an essential component required by enzymes that catalyze diverse key reactions in the global metabolism of carbon, nitrogen and sulfur. The molybdenum-cofactor biosynthesis protein C derived from T. thermophilus was crystallized in two different space groups. Crystals obtained using the first crystallization condition belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 64.81, b = 109.84, c = 115.19 Å, β = 104.9°; the crystal diffracted to a resolution of 1.9 Å. The other crystal form belonged to space group R32, with unit-cell parameters a = b = 106.57, c = 59.25 Å, and diffracted to 1.75 Å resolution. Preliminary calculations reveal that the asymmetric unit contains 12 monomers and one monomer for the crystals belonging to space group P2{sub 1} and R32, respectively.

Authors:
;  [1];  [2];  [2];  [3]; ; ;  [4]; ; ;  [2];  [2];  [3];  [2];  [1];  [5];  [2];  [3];  [3];  [6]
  1. Bioinformatics Centre (Centre of Excellence in Structural Biology and Bio-computing), Indian Institute of Science, Bangalore 560 012 (India)
  2. RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan)
  3. (Japan)
  4. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 (United States)
  5. (India)
  6. (Centre of Excellence in Structural Biology and Bio-computing), Indian Institute of Science, Bangalore 560 012 (India)
Publication Date:
OSTI Identifier:
22360245
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 1; Other Information: PMCID: PMC2330104; PMID: 17183168; PUBLISHER-ID: en5212; OAI: oai:pubmedcentral.nih.gov:2330104; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CARBON; CRYSTALLIZATION; CRYSTALS; ENVIRONMENT; IRON; MOLYBDENUM; MONOMERS; NITROGEN; RESOLUTION; SPACE GROUPS; SULFUR

Citation Formats

Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Chen, Lirong, Liu, Zhi-Jie, Wang, Bi-Cheng, Nishida, Masami, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Shiro, Yoshitsugu, Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in, Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012, Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, and Bioinformatics Centre. Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309106052560.
Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Chen, Lirong, Liu, Zhi-Jie, Wang, Bi-Cheng, Nishida, Masami, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Shiro, Yoshitsugu, Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in, Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012, Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, & Bioinformatics Centre. Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus. United Kingdom. doi:10.1107/S1744309106052560.
Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Chen, Lirong, Liu, Zhi-Jie, Wang, Bi-Cheng, Nishida, Masami, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Shiro, Yoshitsugu, Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in, Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012, Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, and Bioinformatics Centre. Mon . "Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus". United Kingdom. doi:10.1107/S1744309106052560.
@article{osti_22360245,
title = {Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus},
author = {Kanaujia, Shankar Prasad and Ranjani, Chellamuthu Vasuki and Jeyakanthan, Jeyaraman and Baba, Seiki and Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 and Chen, Lirong and Liu, Zhi-Jie and Wang, Bi-Cheng and Nishida, Masami and Ebihara, Akio and Shinkai, Akeo and Kuramitsu, Seiki and Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 and Shiro, Yoshitsugu and Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in and Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012 and Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in and RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045 and Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 and Bioinformatics Centre},
abstractNote = {The molybdenum-cofactor biosynthesis protein C from T. thermophilus has been crystallized in two different space groups, P2{sub 1} and R32; the crystals diffracted to 1.9 and 1.75 Å resolution, respectively. The Gram-negative aerobic eubacterium Thermus thermophilus is an extremely important thermophilic microorganism that was originally isolated from a thermal vent environment in Japan. The molybdenum cofactor in this organism is considered to be an essential component required by enzymes that catalyze diverse key reactions in the global metabolism of carbon, nitrogen and sulfur. The molybdenum-cofactor biosynthesis protein C derived from T. thermophilus was crystallized in two different space groups. Crystals obtained using the first crystallization condition belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 64.81, b = 109.84, c = 115.19 Å, β = 104.9°; the crystal diffracted to a resolution of 1.9 Å. The other crystal form belonged to space group R32, with unit-cell parameters a = b = 106.57, c = 59.25 Å, and diffracted to 1.75 Å resolution. Preliminary calculations reveal that the asymmetric unit contains 12 monomers and one monomer for the crystals belonging to space group P2{sub 1} and R32, respectively.},
doi = {10.1107/S1744309106052560},
journal = {Acta Crystallographica. Section F},
number = Pt 1,
volume = 63,
place = {United Kingdom},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}