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Title: Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya

Abstract

The Kunitz-type trypsin/chymotrypsin inhibitor isolated from C. papaya latex has been crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms are observed, diffracting to 2.6 and 1.7 Å. A Kunitz-type protease inhibitor purified from the latex of green papaya (Carica papaya) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 Å and belongs to space group P3{sub 1} or P3{sub 2}. This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit-cell parameters a = b = 74.70, c = 78.97 Å. Crystal form II, which was grown in the presence of Co{sup 2+}, diffracts to a resolution of 1.7 Å and belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 44.26, b = 81.99, c = 140.89 Å.

Authors:
 [1];  [2];  [1]; ;  [2];  [1]
  1. Université Libre de Bruxelles, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme (CP 609), 808 Route de Lennik, B-1070 Brussels (Belgium)
  2. Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie and Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel (Belgium)
Publication Date:
OSTI Identifier:
22356419
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 62; Journal Issue: Pt 12; Other Information: PMCID: PMC2225369; PMID: 17142906; PUBLISHER-ID: ll5090; OAI: oai:pubmedcentral.nih.gov:2225369; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CATIONS; CRYSTALLIZATION; DIFFUSION; LATEX; METALS; MOLECULES; RESOLUTION; SPACE GROUPS

Citation Formats

Azarkan, Mohamed, Garcia-Pino, Abel, Dibiani, Rachid, Wyns, Lode, Loris, Remy, E-mail: reloris@vub.ac.be, and Baeyens-Volant, Danielle. Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya. United Kingdom: N. p., 2006. Web. doi:10.1107/S1744309106046367.
Azarkan, Mohamed, Garcia-Pino, Abel, Dibiani, Rachid, Wyns, Lode, Loris, Remy, E-mail: reloris@vub.ac.be, & Baeyens-Volant, Danielle. Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya. United Kingdom. doi:10.1107/S1744309106046367.
Azarkan, Mohamed, Garcia-Pino, Abel, Dibiani, Rachid, Wyns, Lode, Loris, Remy, E-mail: reloris@vub.ac.be, and Baeyens-Volant, Danielle. Fri . "Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya". United Kingdom. doi:10.1107/S1744309106046367.
@article{osti_22356419,
title = {Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya},
author = {Azarkan, Mohamed and Garcia-Pino, Abel and Dibiani, Rachid and Wyns, Lode and Loris, Remy, E-mail: reloris@vub.ac.be and Baeyens-Volant, Danielle},
abstractNote = {The Kunitz-type trypsin/chymotrypsin inhibitor isolated from C. papaya latex has been crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms are observed, diffracting to 2.6 and 1.7 Å. A Kunitz-type protease inhibitor purified from the latex of green papaya (Carica papaya) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 Å and belongs to space group P3{sub 1} or P3{sub 2}. This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit-cell parameters a = b = 74.70, c = 78.97 Å. Crystal form II, which was grown in the presence of Co{sup 2+}, diffracts to a resolution of 1.7 Å and belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 44.26, b = 81.99, c = 140.89 Å.},
doi = {10.1107/S1744309106046367},
journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 12,
volume = 62,
place = {United Kingdom},
year = {2006},
month = {12}
}