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Title: Crystallization and X-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72

Abstract

Crystals of 6-aminohexanoate-cyclic-dimer hydrolase were obtained by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. Diffraction data from native and mercury-derivative crystals were collected to resolutions of 1.90 and 2.06 Å, respectively. 6-Aminohexanoate-cyclic-dimer hydrolase (EI) from Arthrobacter sp. KI72 was expressed in Escherichia coli and purified by anion-exchange chromatography. EI was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as precipitant in imidazole buffer pH 8.0. The crystal is hexagonal, with unit-cell parameters a = b = 130.75, c = 58.23 Å. Diffraction data were collected from native and mercury(II) dichloride-derivative crystals to resolutions of 1.90 and 2.06 Å, respectively.

Authors:
;  [1];  [2]; ;  [1];  [2]
  1. Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280 (Japan)
  2. Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297 (Japan)
Publication Date:
OSTI Identifier:
22356408
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 62; Journal Issue: Pt 12; Other Information: PMCID: PMC2225356; PMID: 17142898; PUBLISHER-ID: en5199; OAI: oai:pubmedcentral.nih.gov:2225356; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BIODEGRADATION; BUFFERS; CITRATES; CRYSTALLIZATION; CRYSTALS; DIFFUSION; DIMERS; ESCHERICHIA COLI; MERCURY; RESOLUTION; SODIUM COMPOUNDS; X-RAY DIFFRACTION

Citation Formats

Yasuhira, Kengo, Uedo, Yuki, Shibata, Naoki, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Negoro, Seiji, Takeo, Masahiro, Higuchi, Yoshiki, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, and Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280. Crystallization and X-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72. United Kingdom: N. p., 2006. Web. doi:10.1107/S1744309106045076.
Yasuhira, Kengo, Uedo, Yuki, Shibata, Naoki, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Negoro, Seiji, Takeo, Masahiro, Higuchi, Yoshiki, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, & Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280. Crystallization and X-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72. United Kingdom. https://doi.org/10.1107/S1744309106045076
Yasuhira, Kengo, Uedo, Yuki, Shibata, Naoki, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Negoro, Seiji, Takeo, Masahiro, Higuchi, Yoshiki, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, and Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280. 2006. "Crystallization and X-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72". United Kingdom. https://doi.org/10.1107/S1744309106045076.
@article{osti_22356408,
title = {Crystallization and X-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72},
author = {Yasuhira, Kengo and Uedo, Yuki and Shibata, Naoki and RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248 and Negoro, Seiji and Takeo, Masahiro and Higuchi, Yoshiki and RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248 and Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280},
abstractNote = {Crystals of 6-aminohexanoate-cyclic-dimer hydrolase were obtained by the sitting-drop vapour-diffusion method using sodium citrate as a precipitant. Diffraction data from native and mercury-derivative crystals were collected to resolutions of 1.90 and 2.06 Å, respectively. 6-Aminohexanoate-cyclic-dimer hydrolase (EI) from Arthrobacter sp. KI72 was expressed in Escherichia coli and purified by anion-exchange chromatography. EI was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as precipitant in imidazole buffer pH 8.0. The crystal is hexagonal, with unit-cell parameters a = b = 130.75, c = 58.23 Å. Diffraction data were collected from native and mercury(II) dichloride-derivative crystals to resolutions of 1.90 and 2.06 Å, respectively.},
doi = {10.1107/S1744309106045076},
url = {https://www.osti.gov/biblio/22356408}, journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 12,
volume = 62,
place = {United Kingdom},
year = {Fri Dec 01 00:00:00 EST 2006},
month = {Fri Dec 01 00:00:00 EST 2006}
}