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Title: Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11

Abstract

The crystallization and preliminary X-ray characterization of the polysaccharide lyase family 11 rhamnogalacturonan lyase are presented. Rhamnogalacturonan lyases degrade rhamnogalacturonan I, a major component of pectin, through a β-elimination reaction. YesW from Bacillus subtilis strain 168 is a novel rhamnogalacturonan lyase classified into polysaccharide lyase family 11 (PL-11). The enzyme was crystallized at 293 K using the sitting-drop vapour-diffusion method with 2-methyl-2,4-pentanediol (MPD) as a precipitant. Preliminary X-ray analysis revealed that the YesW crystals belong to space group P2{sub 1} and diffract to 2.40 Å resolution, with unit-cell parameters a = 56.7, b = 105.6, c = 101.4 Å, β = 94.9°. This is the first report on the crystallization and preliminary X-ray analysis of a family PL-11 rhamnogalacturonan lyase.

Authors:
 [1]; ; ;  [2]; ;  [1]
  1. Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011 (Japan)
  2. Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011 (Japan)
Publication Date:
OSTI Identifier:
22356312
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 62; Journal Issue: Pt 5; Other Information: PMCID: PMC2219969; PMID: 16682770; PUBLISHER-ID: en5165; OAI: oai:pubmedcentral.nih.gov:2219969; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFUSION; RESOLUTION; SPACE GROUPS; STRAINS

Citation Formats

Ochiai, Akihito, Yamasaki, Masayuki, Itoh, Takafumi, Mikami, Bunzo, Hashimoto, Wataru, and Murata, Kousaku, E-mail: kmurata@kais.kyoto-u.ac.jp. Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11. United Kingdom: N. p., 2006. Web. doi:10.1107/S1744309106011894.
Ochiai, Akihito, Yamasaki, Masayuki, Itoh, Takafumi, Mikami, Bunzo, Hashimoto, Wataru, & Murata, Kousaku, E-mail: kmurata@kais.kyoto-u.ac.jp. Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11. United Kingdom. doi:10.1107/S1744309106011894.
Ochiai, Akihito, Yamasaki, Masayuki, Itoh, Takafumi, Mikami, Bunzo, Hashimoto, Wataru, and Murata, Kousaku, E-mail: kmurata@kais.kyoto-u.ac.jp. Mon . "Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11". United Kingdom. doi:10.1107/S1744309106011894.
@article{osti_22356312,
title = {Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11},
author = {Ochiai, Akihito and Yamasaki, Masayuki and Itoh, Takafumi and Mikami, Bunzo and Hashimoto, Wataru and Murata, Kousaku, E-mail: kmurata@kais.kyoto-u.ac.jp},
abstractNote = {The crystallization and preliminary X-ray characterization of the polysaccharide lyase family 11 rhamnogalacturonan lyase are presented. Rhamnogalacturonan lyases degrade rhamnogalacturonan I, a major component of pectin, through a β-elimination reaction. YesW from Bacillus subtilis strain 168 is a novel rhamnogalacturonan lyase classified into polysaccharide lyase family 11 (PL-11). The enzyme was crystallized at 293 K using the sitting-drop vapour-diffusion method with 2-methyl-2,4-pentanediol (MPD) as a precipitant. Preliminary X-ray analysis revealed that the YesW crystals belong to space group P2{sub 1} and diffract to 2.40 Å resolution, with unit-cell parameters a = 56.7, b = 105.6, c = 101.4 Å, β = 94.9°. This is the first report on the crystallization and preliminary X-ray analysis of a family PL-11 rhamnogalacturonan lyase.},
doi = {10.1107/S1744309106011894},
journal = {Acta Crystallographica. Section F},
number = Pt 5,
volume = 62,
place = {United Kingdom},
year = {Mon May 01 00:00:00 EDT 2006},
month = {Mon May 01 00:00:00 EDT 2006}
}