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Title: Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds

Abstract

A lectin from C. roseum seeds (CRL) has been purified, characterized and crystallized. A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris–HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2{sub 1}2{sub 1}2{sub 1}. Crystallographic refinement and full amino-acid sequence determination are in progress.

Authors:
; ; ; ;  [1];  [2];  [1];  [3]; ;  [4];  [5];  [6];  [7];  [8]
  1. BioMol-Lab - Department of Biochemistry, Federal University of Ceará (Brazil)
  2. INPA, Manaus, Amazonas (Brazil)
  3. Biomol-Mar-Fishing Engineering Faculty, Federal University of Ceará (Brazil)
  4. Programa de Pós-Graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP (Brazil)
  5. Departamento de Morfofisiologia - CCBS - UFMS, Campo Grande-MS, 79070-900 (Brazil)
  6. Faculdade de Biociências - PUCRS - Porto Alegre-RS, 90619-900 (Brazil)
  7. Laboratoire de Chimie Biologique et Unité Mixte de Recherche No. 8576 du CNRS, Université des Sciences et Technologies de Lille (France)
  8. (Brazil)
Publication Date:
OSTI Identifier:
22356283
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 62; Journal Issue: Pt 3; Other Information: PMCID: PMC2197170; PMID: 16511310; PUBLISHER-ID: en5148; OAI: oai:pubmedcentral.nih.gov:2197170; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALS; DIFFUSION; MATHEMATICAL SOLUTIONS; RESOLUTION; SOLUTIONS; SPACE GROUPS; X-RAY DIFFRACTION

Citation Formats

Cavada, Benildo S., E-mail: bscavada@ufc.br, Marinho, Emmanuel S., Souza, Emmanuel P., Benevides, Raquel G., Delatorre, Plínio, Souza, Luis A. G., Nascimento, Kyria S., Sampaio, Alexandre H., Moreno, Frederico B. M. B., Rustiguel, Joane K. R., Canduri, Fernanda, Azevedo, Walter F. Jr de, E-mail: bscavada@ufc.br, Debray, Henri, and BioMol-Lab - Department of Biochemistry, Federal University of Ceará. Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds. United Kingdom: N. p., 2006. Web. doi:10.1107/S174430910600371X.
Cavada, Benildo S., E-mail: bscavada@ufc.br, Marinho, Emmanuel S., Souza, Emmanuel P., Benevides, Raquel G., Delatorre, Plínio, Souza, Luis A. G., Nascimento, Kyria S., Sampaio, Alexandre H., Moreno, Frederico B. M. B., Rustiguel, Joane K. R., Canduri, Fernanda, Azevedo, Walter F. Jr de, E-mail: bscavada@ufc.br, Debray, Henri, & BioMol-Lab - Department of Biochemistry, Federal University of Ceará. Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds. United Kingdom. doi:10.1107/S174430910600371X.
Cavada, Benildo S., E-mail: bscavada@ufc.br, Marinho, Emmanuel S., Souza, Emmanuel P., Benevides, Raquel G., Delatorre, Plínio, Souza, Luis A. G., Nascimento, Kyria S., Sampaio, Alexandre H., Moreno, Frederico B. M. B., Rustiguel, Joane K. R., Canduri, Fernanda, Azevedo, Walter F. Jr de, E-mail: bscavada@ufc.br, Debray, Henri, and BioMol-Lab - Department of Biochemistry, Federal University of Ceará. Wed . "Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds". United Kingdom. doi:10.1107/S174430910600371X.
@article{osti_22356283,
title = {Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds},
author = {Cavada, Benildo S., E-mail: bscavada@ufc.br and Marinho, Emmanuel S. and Souza, Emmanuel P. and Benevides, Raquel G. and Delatorre, Plínio and Souza, Luis A. G. and Nascimento, Kyria S. and Sampaio, Alexandre H. and Moreno, Frederico B. M. B. and Rustiguel, Joane K. R. and Canduri, Fernanda and Azevedo, Walter F. Jr de, E-mail: bscavada@ufc.br and Debray, Henri and BioMol-Lab - Department of Biochemistry, Federal University of Ceará},
abstractNote = {A lectin from C. roseum seeds (CRL) has been purified, characterized and crystallized. A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris–HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2{sub 1}2{sub 1}2{sub 1}. Crystallographic refinement and full amino-acid sequence determination are in progress.},
doi = {10.1107/S174430910600371X},
journal = {Acta Crystallographica. Section F},
number = Pt 3,
volume = 62,
place = {United Kingdom},
year = {Wed Mar 01 00:00:00 EST 2006},
month = {Wed Mar 01 00:00:00 EST 2006}
}
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