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Title: Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima

Abstract

Cytosolic class II α-mannosidase from T. maritima (TM1851), a family 38 glycoside hydrolase, was crystallized. A diffraction data set was collected to 2.9 Å resolution. Class II α-mannosidase cleaves off α-1,2-, α-1,3- and α-1,6-mannose residues. In this paper, the crystallization and preliminary X-ray analysis of cytosolic class II α-mannosidase from Thermotoga maritima (TM1851), a family 38 glycoside hydrolase, is described. The crystal of recombinant TM1851 belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 244.7, b = 87.4, c = 166.6 Å, β = 124.7°. X-ray diffraction data were collected to a resolution of 2.9 Å.

Authors:
; ; ; ;  [1]
  1. Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)
Publication Date:
OSTI Identifier:
22356269
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 62; Journal Issue: Pt 2; Other Information: PMCID: PMC2150957; PMID: 16511275; PUBLISHER-ID: pu5120; OAI: oai:pubmedcentral.nih.gov:2150957; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; RESOLUTION; SPACE GROUPS; X-RAY DIFFRACTION

Citation Formats

Nakajima, Masahiro, Fushinobu, Shinya, E-mail: asfushi@mail.ecc.u-tokyo.ac.jp, Imamura, Hiromi, Shoun, Hirofumi, and Wakagi, Takayoshi. Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima. United Kingdom: N. p., 2006. Web. doi:10.1107/S1744309105042508.
Nakajima, Masahiro, Fushinobu, Shinya, E-mail: asfushi@mail.ecc.u-tokyo.ac.jp, Imamura, Hiromi, Shoun, Hirofumi, & Wakagi, Takayoshi. Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima. United Kingdom. doi:10.1107/S1744309105042508.
Nakajima, Masahiro, Fushinobu, Shinya, E-mail: asfushi@mail.ecc.u-tokyo.ac.jp, Imamura, Hiromi, Shoun, Hirofumi, and Wakagi, Takayoshi. Wed . "Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima". United Kingdom. doi:10.1107/S1744309105042508.
@article{osti_22356269,
title = {Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima},
author = {Nakajima, Masahiro and Fushinobu, Shinya, E-mail: asfushi@mail.ecc.u-tokyo.ac.jp and Imamura, Hiromi and Shoun, Hirofumi and Wakagi, Takayoshi},
abstractNote = {Cytosolic class II α-mannosidase from T. maritima (TM1851), a family 38 glycoside hydrolase, was crystallized. A diffraction data set was collected to 2.9 Å resolution. Class II α-mannosidase cleaves off α-1,2-, α-1,3- and α-1,6-mannose residues. In this paper, the crystallization and preliminary X-ray analysis of cytosolic class II α-mannosidase from Thermotoga maritima (TM1851), a family 38 glycoside hydrolase, is described. The crystal of recombinant TM1851 belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 244.7, b = 87.4, c = 166.6 Å, β = 124.7°. X-ray diffraction data were collected to a resolution of 2.9 Å.},
doi = {10.1107/S1744309105042508},
journal = {Acta Crystallographica. Section F},
number = Pt 2,
volume = 62,
place = {United Kingdom},
year = {Wed Feb 01 00:00:00 EST 2006},
month = {Wed Feb 01 00:00:00 EST 2006}
}