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Title: Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3

Abstract

A putative ribosomal RNA-processing factor consisting of two KH domains from Pyrococcus horikoshii OT3 (PH1566; 25 kDa) was crystallized by the sitting-drop vapour-diffusion method using PEG 3000 as the precipitant. The space group of the crystals was determined as primitive orthorhombic P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 45.9, b = 47.4, c = 95.7 Å. A putative ribosomal RNA-processing factor consisting of two KH domains from Pyrococcus horikoshii OT3 (PH1566; 25 kDa) was crystallized by the sitting-drop vapour-diffusion method using PEG 3000 as the precipitant. The crystals diffracted X-rays to beyond 2.0 Å resolution using a synchrotron-radiation source. The space group of the crystals was determined as primitive orthorhombic P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 45.9, b = 47.4, c = 95.7 Å. The crystals contain one molecule in the asymmetric unit (V{sub M} = 2.5 Å{sup 3} Da{sup −1}) and have a solvent content of 50%.

Authors:
; ;  [1]; ;  [1];  [2]
  1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)
  2. (Japan)
Publication Date:
OSTI Identifier:
22356251
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 62; Journal Issue: Pt 1; Other Information: PMCID: PMC2150936; PMID: 16511260; PUBLISHER-ID: pu5113; OAI: oai:pubmedcentral.nih.gov:2150936; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFUSION; MOLECULES; PROCESSING; RESOLUTION; SOLVENTS; SPACE GROUPS

Citation Formats

Jia, Min Ze, Ohtsuka, Jun, Lee, Woo Cheol, Nagata, Koji, Tanokura, Masaru, E-mail: amtanok@mail.ecc.u-tokyo.ac.jp, and Biological Supramolecular Crystallography Laboratory, RIKEN Harima Institute at SPring-8, 1-1-1 Koto, Mikadzuki-cho, Sayo-gun, Hyogo 679-5148. Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. United Kingdom: N. p., 2006. Web. doi:10.1107/S1744309105040613.
Jia, Min Ze, Ohtsuka, Jun, Lee, Woo Cheol, Nagata, Koji, Tanokura, Masaru, E-mail: amtanok@mail.ecc.u-tokyo.ac.jp, & Biological Supramolecular Crystallography Laboratory, RIKEN Harima Institute at SPring-8, 1-1-1 Koto, Mikadzuki-cho, Sayo-gun, Hyogo 679-5148. Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. United Kingdom. doi:10.1107/S1744309105040613.
Jia, Min Ze, Ohtsuka, Jun, Lee, Woo Cheol, Nagata, Koji, Tanokura, Masaru, E-mail: amtanok@mail.ecc.u-tokyo.ac.jp, and Biological Supramolecular Crystallography Laboratory, RIKEN Harima Institute at SPring-8, 1-1-1 Koto, Mikadzuki-cho, Sayo-gun, Hyogo 679-5148. Sun . "Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3". United Kingdom. doi:10.1107/S1744309105040613.
@article{osti_22356251,
title = {Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3},
author = {Jia, Min Ze and Ohtsuka, Jun and Lee, Woo Cheol and Nagata, Koji and Tanokura, Masaru, E-mail: amtanok@mail.ecc.u-tokyo.ac.jp and Biological Supramolecular Crystallography Laboratory, RIKEN Harima Institute at SPring-8, 1-1-1 Koto, Mikadzuki-cho, Sayo-gun, Hyogo 679-5148},
abstractNote = {A putative ribosomal RNA-processing factor consisting of two KH domains from Pyrococcus horikoshii OT3 (PH1566; 25 kDa) was crystallized by the sitting-drop vapour-diffusion method using PEG 3000 as the precipitant. The space group of the crystals was determined as primitive orthorhombic P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 45.9, b = 47.4, c = 95.7 Å. A putative ribosomal RNA-processing factor consisting of two KH domains from Pyrococcus horikoshii OT3 (PH1566; 25 kDa) was crystallized by the sitting-drop vapour-diffusion method using PEG 3000 as the precipitant. The crystals diffracted X-rays to beyond 2.0 Å resolution using a synchrotron-radiation source. The space group of the crystals was determined as primitive orthorhombic P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 45.9, b = 47.4, c = 95.7 Å. The crystals contain one molecule in the asymmetric unit (V{sub M} = 2.5 Å{sup 3} Da{sup −1}) and have a solvent content of 50%.},
doi = {10.1107/S1744309105040613},
journal = {Acta Crystallographica. Section F},
number = Pt 1,
volume = 62,
place = {United Kingdom},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}