skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus

Abstract

Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. They belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 Å, β = 108.9°. The asymmetric unit contains one ligase molecule.

Authors:
;  [1];  [2];  [1]
  1. Department of Structural Biology, Biomolecular Engineering Research Institute (BERI), 6-2-3 Furuedai, Suita, Osaka 565-0874 (Japan)
  2. Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581 (Japan)
Publication Date:
OSTI Identifier:
22356193
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 61; Journal Issue: Pt 12; Other Information: PMCID: PMC1978145; PMID: 16511246; PUBLISHER-ID: za5124; OAI: oai:pubmedcentral.nih.gov:1978145; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DNA; ETHANOL; MOLECULES; RECOMBINATION; RESOLUTION; SPACE GROUPS

Citation Formats

Nishida, Hirokazu, Tsuchiya, Daisuke, Ishino, Yoshizumi, and Morikawa, Kosuke, E-mail: morikako@protein.osaka-u.ac.jp. Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus. United Kingdom: N. p., 2005. Web. doi:10.1107/S1744309105038649.
Nishida, Hirokazu, Tsuchiya, Daisuke, Ishino, Yoshizumi, & Morikawa, Kosuke, E-mail: morikako@protein.osaka-u.ac.jp. Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus. United Kingdom. doi:10.1107/S1744309105038649.
Nishida, Hirokazu, Tsuchiya, Daisuke, Ishino, Yoshizumi, and Morikawa, Kosuke, E-mail: morikako@protein.osaka-u.ac.jp. Thu . "Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus". United Kingdom. doi:10.1107/S1744309105038649.
@article{osti_22356193,
title = {Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus},
author = {Nishida, Hirokazu and Tsuchiya, Daisuke and Ishino, Yoshizumi and Morikawa, Kosuke, E-mail: morikako@protein.osaka-u.ac.jp},
abstractNote = {Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates in these events and the sealing of these breaks depends solely on the proper function of DNA ligase. Crystals of the archaeal DNA ligase from Pyrococcus furiosus were obtained using 6.6%(v/v) ethanol as a precipitant and diffracted X-rays to 1.7 Å resolution. They belong to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 61.1, b = 88.3, c = 63.4 Å, β = 108.9°. The asymmetric unit contains one ligase molecule.},
doi = {10.1107/S1744309105038649},
journal = {Acta Crystallographica. Section F},
number = Pt 12,
volume = 61,
place = {United Kingdom},
year = {Thu Dec 01 00:00:00 EST 2005},
month = {Thu Dec 01 00:00:00 EST 2005}
}