Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Journal Article · Tue Nov 01 00:00:00 EST 2005 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309105034330· OSTI ID:22356172

Rhee, Kyung-Hee ^[1]; Lee, Ki Seog ^[2]; Priyadarshi, Amit; Kim, Eunice Eunkyung ^[1]

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1. Biomedical Research Center, Division of Life Sicence, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Sungbuk-gu, Seoul 136-791 (Korea, Republic of)
2. Division of Biotechnology and Genetic Engineering, Korea University, Seoul 136-701 (Korea, Republic of)

Fatty acid-CoA racemase from M. tuberculosis H37Rv has been overexpressed, purified and crystallized. Diffraction data have been collected to beyond 2.7 Å resolution using a synchrotron-radiation source. Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3{sub 1} or P3{sub 2}, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a V{sub M} value of 2.15 Å{sup 3} Da{sup −1}. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.

• OSTI ID: 22356172
• Journal Information: Acta Crystallographica. Section F, Vol. 61, Issue Pt 11; Other Information: PMCID: PMC1978124; PMID: 16511223; PUBLISHER-ID: pu5111; OAI: oai:pubmedcentral.nih.gov:1978124; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CARBOXYLIC ACIDS
CONVERSION
CRYSTALLIZATION
CRYSTALS
DIFFRACTION
DIFFUSION
MONOMERS
OXIDATION
RESOLUTION
SPACE GROUPS