Crystallization and preliminary diffraction analysis of a group I ribozyme from bacteriophage Twort
A group I self-splicing RNA has been synthesized and cocrystallized with a four-nucleotide product RNA. Iodination of the product RNA produces a heavy-atom derivative suitable for structure determination. Group I introns are catalytic RNAs that are capable of performing a variety of phosphotransesterification reactions including self-splicing and RNA cleavage. The reactions are efficient, accurate and dependent only on the presence of guanosine-nucleotide substrate and sufficient magnesium ion to stabilize the structure of the RNA. To understand how the group I intron active-site facilitates catalysis, crystals of a 242-nucleotide ribozyme bound to a four-nucleotide product RNA have been produced that diffract to 3.6 Å resolution. The space group of these crystals is I2{sub 1}2{sub 1}2{sub 1} and the unit-cell parameters are a = 94.6, b = 141.0, c = 210.9 Å. A single heavy-atom derivative has been synthesized by covalent modification of the product RNA with iodine.
- OSTI ID:
- 22356061
- Journal Information:
- Acta Crystallographica. Section F, Vol. 61, Issue Pt 1; Other Information: PMCID: PMC1952367; PMID: 16508095; PUBLISHER-ID: ll5002; OAI: oai:pubmedcentral.nih.gov:1952367; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Introns in the genome of bacteriophage T4
Compaction of a Bacterial Group I Ribozyme Coincides with the Assembly of Core Helices