Crystallization and preliminary crystallographic analysis of endo-1,3-β-glucanase from Arthrobacter sp
- Graduate School of Agriculture, Kyoto Prefectural University, 1-5 Shimogamo, Nakaragi-cho, Sakyo-ku, Kyoto 606-8522 (Japan)
- Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011 (Japan)
Endo-1,3-β-glucanase from Arthrobacter sp. has been crystallized and X-ray diffraction data have been collected to 1.66 Å resolution. Endo-1,3-β-glucanases hydrolyze internal 1,3-β-glucosyl linkages. The endo-1,3-β-glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P4{sub 1}, with unit-cell parameters a = 71.31, c = 60.07 Å, and contained one molecule per asymmetric unit. The Matthews coefficient (V{sub M}) and the solvent content were 2.35 Å{sup 3} Da{sup −1} and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 Å at SPring-8 using a MAR CCD area detector and gave a data set with an overall R{sub merge} of 5.4% and a completeness of 99.4%.
- OSTI ID:
- 22356060
- Journal Information:
- Acta Crystallographica. Section F, Vol. 61, Issue Pt 1; Other Information: PMCID: PMC1952366; PMID: 16508094; PUBLISHER-ID: vr5029; OAI: oai:pubmedcentral.nih.gov:1952366; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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