Nanoflow electrospinning serial femtosecond crystallography
- SLAC National Accelerator Laboratory, Menlo Park, CA 94025 (United States)
- Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States)
- Technische Universität Berlin, Strasse des 17 Juni 135, 10623 Berlin (Germany)
- Stanford University, Stanford, CA 94025 (United States)
- European Synchrotron Radiation Facility, Grenoble (France)
- Umeå Universitet, Umeå (Sweden)
A low flow rate liquid microjet method for delivery of hydrated protein crystals to X-ray lasers is presented. Linac Coherent Light Source data demonstrates serial femtosecond protein crystallography with micrograms, a reduction of sample consumption by orders of magnitude. An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min{sup −1} to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min{sup −1} and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
- OSTI ID:
- 22351266
- Journal Information:
- Acta Crystallographica. Section D: Biological Crystallography, Vol. 68, Issue Pt 11; Other Information: PMCID: PMC3478121; PMID: 23090408; PUBLISHER-ID: lv5021; OAI: oai:pubmedcentral.nih.gov:3478121; Copyright (c) International Union of Crystallography 2012; Country of input: International Atomic Energy Agency (IAEA); ISSN 0907-4449
- Country of Publication:
- Denmark
- Language:
- English
Similar Records
A novel inert crystal delivery medium for serial femtosecond crystallography
Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals