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Title: Nanoflow electrospinning serial femtosecond crystallography

Abstract

A low flow rate liquid microjet method for delivery of hydrated protein crystals to X-ray lasers is presented. Linac Coherent Light Source data demonstrates serial femtosecond protein crystallography with micrograms, a reduction of sample consumption by orders of magnitude. An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min{sup −1} to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min{sup −1} and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

Authors:
;  [1];  [2];  [3]; ;  [2];  [1];  [2]; ;  [4];  [1]; ; ;  [2];  [1];  [5];  [6]; ; ; more »; ; ; ; ; ;  [1];  [2];  [7]; ;  [1];  [2]; ;  [1];  [4];  [8];  [2];  [1]; ;  [2];  [1];  [3] « less
  1. SLAC National Accelerator Laboratory, Menlo Park, CA 94025 (United States)
  2. Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States)
  3. (United States)
  4. Technische Universität Berlin, Strasse des 17 Juni 135, 10623 Berlin (Germany)
  5. (Sweden)
  6. Stanford University, Stanford, CA 94025 (United States)
  7. European Synchrotron Radiation Facility, Grenoble (France)
  8. Umeå Universitet, Umeå (Sweden)
Publication Date:
OSTI Identifier:
22351266
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section D: Biological Crystallography
Additional Journal Information:
Journal Volume: 68; Journal Issue: Pt 11; Other Information: PMCID: PMC3478121; PMID: 23090408; PUBLISHER-ID: lv5021; OAI: oai:pubmedcentral.nih.gov:3478121; Copyright (c) International Union of Crystallography 2012; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0907-4449
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLOGRAPHY; CRYSTALS; DELIVERY; DIFFRACTION; FLOW RATE; LIGHT SOURCES; LINEAR ACCELERATORS; LIQUIDS; PROTEINS; REDUCTION; RESOLUTION; SPIN; SUSPENSIONS; X-RAY LASERS

Citation Formats

Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, Stockholm University, S-106 91 Stockholm, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J., E-mail: mbogan@slac.stanford.edu, and SLAC National Accelerator Laboratory, Menlo Park, CA 94025. Nanoflow electrospinning serial femtosecond crystallography. Denmark: N. p., 2012. Web. doi:10.1107/S0907444912038152.
Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, Stockholm University, S-106 91 Stockholm, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J., E-mail: mbogan@slac.stanford.edu, & SLAC National Accelerator Laboratory, Menlo Park, CA 94025. Nanoflow electrospinning serial femtosecond crystallography. Denmark. doi:10.1107/S0907444912038152.
Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, Stockholm University, S-106 91 Stockholm, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J., E-mail: mbogan@slac.stanford.edu, and SLAC National Accelerator Laboratory, Menlo Park, CA 94025. Thu . "Nanoflow electrospinning serial femtosecond crystallography". Denmark. doi:10.1107/S0907444912038152.
@article{osti_22351266,
title = {Nanoflow electrospinning serial femtosecond crystallography},
author = {Sierra, Raymond G. and Laksmono, Hartawan and Kern, Jan and SLAC National Accelerator Laboratory, Menlo Park, CA 94025 and Tran, Rosalie and Hattne, Johan and Alonso-Mori, Roberto and Lassalle-Kaiser, Benedikt and Glöckner, Carina and Hellmich, Julia and Schafer, Donald W. and Echols, Nathaniel and Gildea, Richard J. and Grosse-Kunstleve, Ralf W. and Sellberg, Jonas and Stockholm University, S-106 91 Stockholm and McQueen, Trevor A. and Fry, Alan R. and Messerschmidt, Marc M. and Miahnahri, Alan and Seibert, M. Marvin and Hampton, Christina Y. and Starodub, Dmitri and Loh, N. Duane and Sokaras, Dimosthenis and Weng, Tsu-Chien and Zwart, Petrus H. and Glatzel, Pieter and Milathianaki, Despina and White, William E. and Adams, Paul D. and Williams, Garth J. and Boutet, Sébastien and Zouni, Athina and Messinger, Johannes and Sauter, Nicholas K. and Bergmann, Uwe and Yano, Junko and Yachandra, Vittal K. and Bogan, Michael J., E-mail: mbogan@slac.stanford.edu and SLAC National Accelerator Laboratory, Menlo Park, CA 94025},
abstractNote = {A low flow rate liquid microjet method for delivery of hydrated protein crystals to X-ray lasers is presented. Linac Coherent Light Source data demonstrates serial femtosecond protein crystallography with micrograms, a reduction of sample consumption by orders of magnitude. An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min{sup −1} to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min{sup −1} and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.},
doi = {10.1107/S0907444912038152},
journal = {Acta Crystallographica. Section D: Biological Crystallography},
issn = {0907-4449},
number = Pt 11,
volume = 68,
place = {Denmark},
year = {2012},
month = {11}
}