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Title: Automating the application of smart materials for protein crystallization

Abstract

The first semi-liquid, non-protein nucleating agent for automated protein crystallization trials is described. This ‘smart material’ is demonstrated to induce crystal growth and will provide a simple, cost-effective tool for scientists in academia and industry. The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as ‘smart materials’) for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials.

Authors:
;  [1]; ;  [2];  [1]
  1. Imperial College London, London SW7 2AZ (United Kingdom)
  2. University of Surrey, Guildford, Surrey GU2 7XH (United Kingdom)
Publication Date:
OSTI Identifier:
22347720
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 71; Journal Issue: Pt 3; Other Information: PMCID: PMC4356364; PMID: 25760603; PUBLISHER-ID: bw5424; OAI: oai:pubmedcentral.nih.gov:4356364; Copyright (c) Khurshid et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTAL GROWTH; CRYSTALLIZATION; CRYSTALS; FABRICATION; LIQUIDS; MATERIALS; POLYMERS; PROBABILITY; PROTEINS; SCREENING; TEXTURE; VALIDATION

Citation Formats

Khurshid, Sahir, Govada, Lata, EL-Sharif, Hazim F., Reddy, Subrayal M., and Chayen, Naomi E., E-mail: n.chayen@imperial.ac.uk. Automating the application of smart materials for protein crystallization. Denmark: N. p., 2015. Web. doi:10.1107/S1399004714027643.
Khurshid, Sahir, Govada, Lata, EL-Sharif, Hazim F., Reddy, Subrayal M., & Chayen, Naomi E., E-mail: n.chayen@imperial.ac.uk. Automating the application of smart materials for protein crystallization. Denmark. doi:10.1107/S1399004714027643.
Khurshid, Sahir, Govada, Lata, EL-Sharif, Hazim F., Reddy, Subrayal M., and Chayen, Naomi E., E-mail: n.chayen@imperial.ac.uk. Sun . "Automating the application of smart materials for protein crystallization". Denmark. doi:10.1107/S1399004714027643.
@article{osti_22347720,
title = {Automating the application of smart materials for protein crystallization},
author = {Khurshid, Sahir and Govada, Lata and EL-Sharif, Hazim F. and Reddy, Subrayal M. and Chayen, Naomi E., E-mail: n.chayen@imperial.ac.uk},
abstractNote = {The first semi-liquid, non-protein nucleating agent for automated protein crystallization trials is described. This ‘smart material’ is demonstrated to induce crystal growth and will provide a simple, cost-effective tool for scientists in academia and industry. The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as ‘smart materials’) for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials.},
doi = {10.1107/S1399004714027643},
journal = {Acta Crystallographica. Section D: Biological Crystallography},
number = Pt 3,
volume = 71,
place = {Denmark},
year = {Sun Mar 01 00:00:00 EST 2015},
month = {Sun Mar 01 00:00:00 EST 2015}
}