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Title: A national facility for biological cryo-electron microscopy

Abstract

This review provides a brief update on the use of cryo-electron microscopy for integrated structural biology, along with an overview of the plans for the UK national facility for electron microscopy being built at the Diamond synchrotron. Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an understanding of the molecular machinery of cells, disease processes and the actions of pathogenic organisms from atomic detail through to the cellular context. However, cutting-edge research in this field requires very substantial resources for equipment, infrastructure and expertise. Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. State-of-the-art equipment operated with expert support will be provided, optimized for both atomic-level single-particle analysis of purified macromolecules and complexes and for tomography of cell sections. The access to and organization of the facility will be modelled on the highly successful macromolecular crystallography (MX) synchrotron beamlines, and will be embedded at the Diamond Light Source, facilitating the development of user-friendly workflows providing near-real-time experimental feedback.

Authors:
 [1];  [2];  [2];  [3];  [3]
  1. Birkbeck College, Malet Street, London WC1E 7HX (United Kingdom)
  2. University of Oxford, Oxford OX3 7BN (United Kingdom)
  3. (United Kingdom)
Publication Date:
OSTI Identifier:
22347705
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 71; Journal Issue: Pt 1; Other Information: PMCID: PMC4304693; PMID: 25615867; PUBLISHER-ID: ba5227; OAI: oai:pubmedcentral.nih.gov:4304693; Copyright (c) Saibil et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ATOMS; CRYSTALS; CUTTING; DIAMONDS; ELECTRON MICROSCOPY; ELECTRONS; FEEDBACK; LIGHT SOURCES; SYNCHROTRONS; TOMOGRAPHY

Citation Formats

Saibil, Helen R., E-mail: h.saibil@mail.cryst.bbk.ac.uk, Grünewald, Kay, Stuart, David I., Diamond Light Source, Didcot OX11 0DE, and Birkbeck College, Malet Street, London WC1E 7HX. A national facility for biological cryo-electron microscopy. Denmark: N. p., 2015. Web. doi:10.1107/S1399004714025280.
Saibil, Helen R., E-mail: h.saibil@mail.cryst.bbk.ac.uk, Grünewald, Kay, Stuart, David I., Diamond Light Source, Didcot OX11 0DE, & Birkbeck College, Malet Street, London WC1E 7HX. A national facility for biological cryo-electron microscopy. Denmark. doi:10.1107/S1399004714025280.
Saibil, Helen R., E-mail: h.saibil@mail.cryst.bbk.ac.uk, Grünewald, Kay, Stuart, David I., Diamond Light Source, Didcot OX11 0DE, and Birkbeck College, Malet Street, London WC1E 7HX. Thu . "A national facility for biological cryo-electron microscopy". Denmark. doi:10.1107/S1399004714025280.
@article{osti_22347705,
title = {A national facility for biological cryo-electron microscopy},
author = {Saibil, Helen R., E-mail: h.saibil@mail.cryst.bbk.ac.uk and Grünewald, Kay and Stuart, David I. and Diamond Light Source, Didcot OX11 0DE and Birkbeck College, Malet Street, London WC1E 7HX},
abstractNote = {This review provides a brief update on the use of cryo-electron microscopy for integrated structural biology, along with an overview of the plans for the UK national facility for electron microscopy being built at the Diamond synchrotron. Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an understanding of the molecular machinery of cells, disease processes and the actions of pathogenic organisms from atomic detail through to the cellular context. However, cutting-edge research in this field requires very substantial resources for equipment, infrastructure and expertise. Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. State-of-the-art equipment operated with expert support will be provided, optimized for both atomic-level single-particle analysis of purified macromolecules and complexes and for tomography of cell sections. The access to and organization of the facility will be modelled on the highly successful macromolecular crystallography (MX) synchrotron beamlines, and will be embedded at the Diamond Light Source, facilitating the development of user-friendly workflows providing near-real-time experimental feedback.},
doi = {10.1107/S1399004714025280},
journal = {Acta Crystallographica. Section D: Biological Crystallography},
number = Pt 1,
volume = 71,
place = {Denmark},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}