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Title: Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines

Abstract

Scaffold/matrix attachment regions (S/MARs) are potential element that can be integrated into expression vector to increase expression of recombinant protein. Many studies on S/MAR have been done but none has revealed the distribution of S/MAR in a genome. In this study, we have isolated S/MAR sequences from HEK293 and Chinese hamster ovary cell lines (CHO DG44) using two different methods utilizing 2 M NaCl and lithium-3,5-diiodosalicylate (LIS). The isolated S/MARs were sequenced using Next Generation Sequencing (NGS) platform. Based on reference mapping analysis against human genome database, a total of 8,994,856 and 8,412,672 contigs of S/MAR sequences were retrieved from 2M NaCl and LIS extraction of HEK293 respectively. On the other hand, reference mapping analysis of S/MAR derived from CHO DG44 against our own CHO DG44 database have generated a total of 7,204,348 and 4,672,913 contigs from 2 M NaCl and LIS extraction method respectively.

Authors:
; ;  [1];  [2]
  1. School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia)
  2. Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia)
Publication Date:
OSTI Identifier:
22308343
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1614; Journal Issue: 1; Conference: 2014 UKM FST postgraduate colloquium, Selangor (Malaysia), 9-11 Apr 2014; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; CHO CELLS; DISTRIBUTION; EXTRACTION; HUMAN POPULATIONS; MAPPING; MATRICES; PROTEINS; SODIUM CHLORIDES; VECTORS

Citation Formats

Pilus, Nur Shazwani Mohd, Ahmad, Azrin, Yusof, Nurul Yuziana Mohd, and Johari, Norazfa. Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines. United States: N. p., 2014. Web. doi:10.1063/1.4895256.
Pilus, Nur Shazwani Mohd, Ahmad, Azrin, Yusof, Nurul Yuziana Mohd, & Johari, Norazfa. Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines. United States. doi:10.1063/1.4895256.
Pilus, Nur Shazwani Mohd, Ahmad, Azrin, Yusof, Nurul Yuziana Mohd, and Johari, Norazfa. Wed . "Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines". United States. doi:10.1063/1.4895256.
@article{osti_22308343,
title = {Mapping analysis of scaffold/matrix attachment regions (s/MARs) from two different mammalian cell lines},
author = {Pilus, Nur Shazwani Mohd and Ahmad, Azrin and Yusof, Nurul Yuziana Mohd and Johari, Norazfa},
abstractNote = {Scaffold/matrix attachment regions (S/MARs) are potential element that can be integrated into expression vector to increase expression of recombinant protein. Many studies on S/MAR have been done but none has revealed the distribution of S/MAR in a genome. In this study, we have isolated S/MAR sequences from HEK293 and Chinese hamster ovary cell lines (CHO DG44) using two different methods utilizing 2 M NaCl and lithium-3,5-diiodosalicylate (LIS). The isolated S/MARs were sequenced using Next Generation Sequencing (NGS) platform. Based on reference mapping analysis against human genome database, a total of 8,994,856 and 8,412,672 contigs of S/MAR sequences were retrieved from 2M NaCl and LIS extraction of HEK293 respectively. On the other hand, reference mapping analysis of S/MAR derived from CHO DG44 against our own CHO DG44 database have generated a total of 7,204,348 and 4,672,913 contigs from 2 M NaCl and LIS extraction method respectively.},
doi = {10.1063/1.4895256},
journal = {AIP Conference Proceedings},
number = 1,
volume = 1614,
place = {United States},
year = {Wed Sep 03 00:00:00 EDT 2014},
month = {Wed Sep 03 00:00:00 EDT 2014}
}
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