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Title: Bidirectional regulation of bakuchiol, an estrogenic-like compound, on catecholamine secretion

Abstract

Excess or deficiency of catecholamine (CA) secretion was related with several diseases. Recently, estrogen and phytoestrogens were reported to regulate the activity of CA system. Bakuchiol is a phytoestrogen isolated from the seeds of Psoralea corylifolia L. (Leguminosae) which has been used in Traditional Chinese medicine as a tonic or aphrodisiac. In the present study, bovine adrenal medullary cells were employed to investigate the effects and mechanisms of bakuchiol on the regulation of CA secretion. Further, its anti-depressant like and anti-stress effects were evaluated by using behavioral despair and chronic immobilization stress models. Our results indicated that bakuchiol showed bidirectional regulation on CA secretion. It stimulated basal CA secretion in a concentration dependent manner (p < 0.01), while it reduced 300 μM acetylcholine (ACh) (p < 0.01), 100 μM veratridine (Ver) (p < 0.01) and 56 mM K{sup +} (p < 0.05) induced CA secretion, respectively. We also found that the stimulation of basal CA secretion by bakuchiol may act through estrogen-like effect and the JNK pathway in an extra-cellular calcium independent manner. Further, bakuchiol elevated tyrosine hydroxylase Ser40 and Ser31 phosphorylation (p < 0.01) through the PKA and ERK1/2 pathways, respectively. Bakuchiol inhibited ACh, Ver and 56 mM K{supmore » +} induced CA secretion was related with reduction of intracellular calcium rise. In vivo experiments, we found that bakuchiol significantly reduced immobilization time in behavioral despair mouse (p < 0.05 or 0.01), and plasma epinephrine (E) and norepinephrine (NE) levels in chronic immobilization stress (p < 0.05). Overall, these results present a bidirectional regulation of bakuchiol on CA secretion which indicated that bakuchiol may exert anti-stress and the potential anti-depressant-like effects. - Highlights: • Bakuchiol stimulated basal catecholamine secretion. • Bakuchiol inhibited various secretagogues induced catecholamine secretion. • Bakuchiol may have anti-stress and the potential anti-depression-like effects.« less

Authors:
; ; ; ; ;  [1];  [2];  [3]; ;  [1];  [2];  [1];  [2]
  1. Tianjin State Key Laboratory of Modern Chinese Medicine, Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae (China)
  2. (China)
  3. First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin (China)
Publication Date:
OSTI Identifier:
22285568
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 274; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACETYLCHOLINE; ADRENALINE; CALCIUM; CATECHOLAMINES; DOPAMINE; ESTROGENS; HYDROXYLASES; LEGUMINOSAE; MICE; NORADRENALINE; POTASSIUM IONS; SECRETION; TYROSINE

Citation Formats

Mao, Haoping, Wang, Hong, Ma, Shangwei, Xu, Yantong, Zhang, Han, Wang, Yuefei, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, Niu, Zichang, Fan, Guanwei, Zhu, Yan, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, Gao, Xiu Mei, E-mail: gaoxiumei@tjutcm.edu.cn, and Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin. Bidirectional regulation of bakuchiol, an estrogenic-like compound, on catecholamine secretion. United States: N. p., 2014. Web. doi:10.1016/J.TAAP.2013.11.001.
Mao, Haoping, Wang, Hong, Ma, Shangwei, Xu, Yantong, Zhang, Han, Wang, Yuefei, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, Niu, Zichang, Fan, Guanwei, Zhu, Yan, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, Gao, Xiu Mei, E-mail: gaoxiumei@tjutcm.edu.cn, & Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin. Bidirectional regulation of bakuchiol, an estrogenic-like compound, on catecholamine secretion. United States. doi:10.1016/J.TAAP.2013.11.001.
Mao, Haoping, Wang, Hong, Ma, Shangwei, Xu, Yantong, Zhang, Han, Wang, Yuefei, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, Niu, Zichang, Fan, Guanwei, Zhu, Yan, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, Gao, Xiu Mei, E-mail: gaoxiumei@tjutcm.edu.cn, and Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin. Wed . "Bidirectional regulation of bakuchiol, an estrogenic-like compound, on catecholamine secretion". United States. doi:10.1016/J.TAAP.2013.11.001.
@article{osti_22285568,
title = {Bidirectional regulation of bakuchiol, an estrogenic-like compound, on catecholamine secretion},
author = {Mao, Haoping and Wang, Hong and Ma, Shangwei and Xu, Yantong and Zhang, Han and Wang, Yuefei and Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin and Niu, Zichang and Fan, Guanwei and Zhu, Yan and Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin and Gao, Xiu Mei, E-mail: gaoxiumei@tjutcm.edu.cn and Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin},
abstractNote = {Excess or deficiency of catecholamine (CA) secretion was related with several diseases. Recently, estrogen and phytoestrogens were reported to regulate the activity of CA system. Bakuchiol is a phytoestrogen isolated from the seeds of Psoralea corylifolia L. (Leguminosae) which has been used in Traditional Chinese medicine as a tonic or aphrodisiac. In the present study, bovine adrenal medullary cells were employed to investigate the effects and mechanisms of bakuchiol on the regulation of CA secretion. Further, its anti-depressant like and anti-stress effects were evaluated by using behavioral despair and chronic immobilization stress models. Our results indicated that bakuchiol showed bidirectional regulation on CA secretion. It stimulated basal CA secretion in a concentration dependent manner (p < 0.01), while it reduced 300 μM acetylcholine (ACh) (p < 0.01), 100 μM veratridine (Ver) (p < 0.01) and 56 mM K{sup +} (p < 0.05) induced CA secretion, respectively. We also found that the stimulation of basal CA secretion by bakuchiol may act through estrogen-like effect and the JNK pathway in an extra-cellular calcium independent manner. Further, bakuchiol elevated tyrosine hydroxylase Ser40 and Ser31 phosphorylation (p < 0.01) through the PKA and ERK1/2 pathways, respectively. Bakuchiol inhibited ACh, Ver and 56 mM K{sup +} induced CA secretion was related with reduction of intracellular calcium rise. In vivo experiments, we found that bakuchiol significantly reduced immobilization time in behavioral despair mouse (p < 0.05 or 0.01), and plasma epinephrine (E) and norepinephrine (NE) levels in chronic immobilization stress (p < 0.05). Overall, these results present a bidirectional regulation of bakuchiol on CA secretion which indicated that bakuchiol may exert anti-stress and the potential anti-depressant-like effects. - Highlights: • Bakuchiol stimulated basal catecholamine secretion. • Bakuchiol inhibited various secretagogues induced catecholamine secretion. • Bakuchiol may have anti-stress and the potential anti-depression-like effects.},
doi = {10.1016/J.TAAP.2013.11.001},
journal = {Toxicology and Applied Pharmacology},
number = 1,
volume = 274,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 2014},
month = {Wed Jan 01 00:00:00 EST 2014}
}
  • The decidualized endometrium during the first trimester of pregnancy synthesizes and secretes a 32-kDa insulin-like growth factor-binding protein (termed hIGFBP-1) at high levels. IGFBP-1 is the major soluble protein product of this tissue and is principally localized to the differentiated endometrial stromal cell, the decidual cell. In the present study long term culture of stromal cells from the nonpregnant endometrium have been employed to elucidate the hormonal requirements for IGFBP-1 production. Immunoreactive IGFBP-1 was undetectable in control cultures. However, inclusion of medroxyprogesterone acetate (MPA) induced rates of 0.35 +/- 0.09 microgram/0.1 mg cell DNA.day after 20-30 days. In these culturesmore » cells exhibited morphological changes consistent with decidual cell differentiation. In all cultures removal of MPA after exposure for 10-16 days, with or without subsequent inclusion of relaxin (RLX), increased production of IGFBP-1 450- to 4600-fold to rates of 150-710 micrograms/0.1 mg cell DNA.day or 26-131 micrograms/10(6) cells.day on days 24-26. The rates tended to be higher with the inclusion of RLX and were sustained in contrast to cultures without RLX, where rates fell by day 30. Individual cultures responded differently to RLX when added from the initiation of culture, with either a response similar to MPA alone or a cyclical change in production, achieving maximal rates of 190-290 micrograms/0.1 mg cell DNA.day. Cultures in which RLX alone induced high IGFBP-1 high production were obtained from endometrium during the progesterone-dominated luteal phase. In cultures exhibiting high rates of immunoreactive IGFBP-1 production, the protein represented their major secretory protein product. This was confirmed by ({sup 35}S)methionine incorporation and the presence of IGFBP-1 as the predominant protein in serum-free culture medium.« less
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