skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films

Abstract

In previous work, we developed novel antibacterial hybrid coatings based on dextran containing dispersed Ag NPs (∼ 5 nm, DEX-Ag) aimed to offer dual protection against two of the most common complications associated with implant surgery, infections and rejection of the implant. However, their blood-material interactions are unknown. In this study, we assess the hemocompatibility and biocompatibility of DEX-Ag using fresh blood and two cell lines of the immune system, monocytes (THP-1 cells) and macrophages (PMA-stimulated THP-1 cells). Glass, polyurethane (PU) and bare dextran (DEX) were used as reference surfaces. PU, DEX and DEX-Ag exhibited non-hemolytic properties. Relative to glass (100%), platelet attachment on PU, DEX and DEX-Ag was 15%, 10% and 34%, respectively. Further, we assessed cell morphology and viability, pro-inflammatory cytokines expression (TNF-α and IL-1β), pro-inflammatory eicosanoid expression (Prostaglandin E{sub 2}, PGE{sub 2}) and release of reactive oxygen species (ROS, superoxide and H{sub 2}O{sub 2}) following incubation of the cells with the surfaces. The morphology and cell viability of THP-1 cells were not affected by DEX-Ag whereas DEX-Ag minimized spreading of PMA-stimulated THP-1 cells and caused a reduction in cell viability (16% relative to other surfaces). Although DEX-Ag slightly enhanced release of ROS, the expression of pro-inflammatory cytokinesmore » remained minimal with similar levels of PGE{sub 2}, as compared to the other surfaces studied. These results highlight low toxicity of DEX-Ag and hold promise for future applications in vivo. - Highlights: • We examined specific blood-contact reactions of dextran doped with Ag NPs coatings. • Biocompatibility was assessed with THP-1 cells and PMA-stimulated THP-1 cells. • Glass, polyurethane and dextran were used as reference surfaces. • Hybrid coatings exhibited non-hemolytic properties. • Low toxicity, inflammatory response and ROS suggest potential for in vivo use.« less

Authors:
 [1];  [1];  [2]
  1. Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA 19104 (United States)
  2. Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104 (United States)
Publication Date:
OSTI Identifier:
22285462
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 272; Journal Issue: 3; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; COATINGS; DEXTRAN; HYBRIDIZATION; HYDROGEN PEROXIDE; INFLAMMATION; LYMPHOKINES; MACROPHAGES; MONOCYTES; NANOSTRUCTURES; OXYGEN; POLYURETHANES; SILVER; SURFACES

Citation Formats

Coll Ferrer, M. Carme, Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104, Eckmann, Uriel N., Composto, Russell J., and Eckmann, David M., E-mail: eckmanndm@uphs.upenn.edu. Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films. United States: N. p., 2013. Web. doi:10.1016/J.TAAP.2013.07.023.
Coll Ferrer, M. Carme, Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104, Eckmann, Uriel N., Composto, Russell J., & Eckmann, David M., E-mail: eckmanndm@uphs.upenn.edu. Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films. United States. https://doi.org/10.1016/J.TAAP.2013.07.023
Coll Ferrer, M. Carme, Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104, Eckmann, Uriel N., Composto, Russell J., and Eckmann, David M., E-mail: eckmanndm@uphs.upenn.edu. Fri . "Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films". United States. https://doi.org/10.1016/J.TAAP.2013.07.023.
@article{osti_22285462,
title = {Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films},
author = {Coll Ferrer, M. Carme and Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104 and Eckmann, Uriel N. and Composto, Russell J. and Eckmann, David M., E-mail: eckmanndm@uphs.upenn.edu},
abstractNote = {In previous work, we developed novel antibacterial hybrid coatings based on dextran containing dispersed Ag NPs (∼ 5 nm, DEX-Ag) aimed to offer dual protection against two of the most common complications associated with implant surgery, infections and rejection of the implant. However, their blood-material interactions are unknown. In this study, we assess the hemocompatibility and biocompatibility of DEX-Ag using fresh blood and two cell lines of the immune system, monocytes (THP-1 cells) and macrophages (PMA-stimulated THP-1 cells). Glass, polyurethane (PU) and bare dextran (DEX) were used as reference surfaces. PU, DEX and DEX-Ag exhibited non-hemolytic properties. Relative to glass (100%), platelet attachment on PU, DEX and DEX-Ag was 15%, 10% and 34%, respectively. Further, we assessed cell morphology and viability, pro-inflammatory cytokines expression (TNF-α and IL-1β), pro-inflammatory eicosanoid expression (Prostaglandin E{sub 2}, PGE{sub 2}) and release of reactive oxygen species (ROS, superoxide and H{sub 2}O{sub 2}) following incubation of the cells with the surfaces. The morphology and cell viability of THP-1 cells were not affected by DEX-Ag whereas DEX-Ag minimized spreading of PMA-stimulated THP-1 cells and caused a reduction in cell viability (16% relative to other surfaces). Although DEX-Ag slightly enhanced release of ROS, the expression of pro-inflammatory cytokines remained minimal with similar levels of PGE{sub 2}, as compared to the other surfaces studied. These results highlight low toxicity of DEX-Ag and hold promise for future applications in vivo. - Highlights: • We examined specific blood-contact reactions of dextran doped with Ag NPs coatings. • Biocompatibility was assessed with THP-1 cells and PMA-stimulated THP-1 cells. • Glass, polyurethane and dextran were used as reference surfaces. • Hybrid coatings exhibited non-hemolytic properties. • Low toxicity, inflammatory response and ROS suggest potential for in vivo use.},
doi = {10.1016/J.TAAP.2013.07.023},
url = {https://www.osti.gov/biblio/22285462}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 272,
place = {United States},
year = {2013},
month = {11}
}