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Title: Myosin heavy chain-like localizes at cell contact sites during Drosophila myoblast fusion and interacts in vitro with Rolling pebbles 7

Abstract

Besides representing the sarcomeric thick filaments, myosins are involved in many cellular transport and motility processes. Myosin heavy chains are grouped into 18 classes. Here we show that in Drosophila, the unconventional group XVIII myosin heavy chain-like (Mhcl) is transcribed in the mesoderm of embryos, most prominently in founder cells (FCs). An ectopically expressed GFP-tagged Mhcl localizes in the growing muscle at cell–cell contacts towards the attached fusion competent myoblast (FCM). We further show that Mhcl interacts in vitro with the essential fusion protein Rolling pebbles 7 (Rols7), which is part of a protein complex established at cell contact sites (Fusion-restricted Myogenic-Adhesive Structure or FuRMAS). Here, branched F-actin is likely needed to widen the fusion pore and to integrate the myoblast into the growing muscle. We show that the localization of Mhcl is dependent on the presence of Rols7, and we postulate that Mhcl acts at the FuRMAS as an actin motor protein. We further show that Mhcl deficient embryos develop a wild-type musculature. We thus propose that Mhcl functions redundantly to other myosin heavy chains in myoblasts. Lastly, we found that the protein is detectable adjacent to the sarcomeric Z-discs, suggesting an additional function in mature muscles. - Highlights:more » ► The class XVIII myosin encoding gene Mhcl is transcribed in the mesoderm. ► Mhcl localization at contact sites of fusing myoblasts depends on Rols7. ► Mhcl interacts in vitro with Rols7 which is essential for myogenesis. ► Functional redundancy with other myosins is likely as mutants show no muscle defects. ► Mhcl localizes adjacent to Z-discs of sarcomeres and might support muscle integrity.« less

Authors:
; ; ; ; ; ;  [1];  [1]
  1. Developmental Biology, Department of Biology, Philipps-Universität Marburg, Karl-von-Frisch-Strasse 8, 35037 Marburg (Germany)
Publication Date:
OSTI Identifier:
22278210
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 319; Journal Issue: 4; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; DROSOPHILA; EMBRYOS; IN VITRO; MYOBLASTS; MYOSIN

Citation Formats

Bonn, Bettina R., Rudolf, Anja, Hornbruch-Freitag, Christina, Daum, Gabor, Kuckwa, Jessica, Kastl, Lena, Buttgereit, Detlev, and Renkawitz-Pohl, Renate, E-mail: renkawit@biologie.uni-marburg.de. Myosin heavy chain-like localizes at cell contact sites during Drosophila myoblast fusion and interacts in vitro with Rolling pebbles 7. United States: N. p., 2013. Web. doi:10.1016/J.YEXCR.2012.12.005.
Bonn, Bettina R., Rudolf, Anja, Hornbruch-Freitag, Christina, Daum, Gabor, Kuckwa, Jessica, Kastl, Lena, Buttgereit, Detlev, & Renkawitz-Pohl, Renate, E-mail: renkawit@biologie.uni-marburg.de. Myosin heavy chain-like localizes at cell contact sites during Drosophila myoblast fusion and interacts in vitro with Rolling pebbles 7. United States. doi:10.1016/J.YEXCR.2012.12.005.
Bonn, Bettina R., Rudolf, Anja, Hornbruch-Freitag, Christina, Daum, Gabor, Kuckwa, Jessica, Kastl, Lena, Buttgereit, Detlev, and Renkawitz-Pohl, Renate, E-mail: renkawit@biologie.uni-marburg.de. Fri . "Myosin heavy chain-like localizes at cell contact sites during Drosophila myoblast fusion and interacts in vitro with Rolling pebbles 7". United States. doi:10.1016/J.YEXCR.2012.12.005.
@article{osti_22278210,
title = {Myosin heavy chain-like localizes at cell contact sites during Drosophila myoblast fusion and interacts in vitro with Rolling pebbles 7},
author = {Bonn, Bettina R. and Rudolf, Anja and Hornbruch-Freitag, Christina and Daum, Gabor and Kuckwa, Jessica and Kastl, Lena and Buttgereit, Detlev and Renkawitz-Pohl, Renate, E-mail: renkawit@biologie.uni-marburg.de},
abstractNote = {Besides representing the sarcomeric thick filaments, myosins are involved in many cellular transport and motility processes. Myosin heavy chains are grouped into 18 classes. Here we show that in Drosophila, the unconventional group XVIII myosin heavy chain-like (Mhcl) is transcribed in the mesoderm of embryos, most prominently in founder cells (FCs). An ectopically expressed GFP-tagged Mhcl localizes in the growing muscle at cell–cell contacts towards the attached fusion competent myoblast (FCM). We further show that Mhcl interacts in vitro with the essential fusion protein Rolling pebbles 7 (Rols7), which is part of a protein complex established at cell contact sites (Fusion-restricted Myogenic-Adhesive Structure or FuRMAS). Here, branched F-actin is likely needed to widen the fusion pore and to integrate the myoblast into the growing muscle. We show that the localization of Mhcl is dependent on the presence of Rols7, and we postulate that Mhcl acts at the FuRMAS as an actin motor protein. We further show that Mhcl deficient embryos develop a wild-type musculature. We thus propose that Mhcl functions redundantly to other myosin heavy chains in myoblasts. Lastly, we found that the protein is detectable adjacent to the sarcomeric Z-discs, suggesting an additional function in mature muscles. - Highlights: ► The class XVIII myosin encoding gene Mhcl is transcribed in the mesoderm. ► Mhcl localization at contact sites of fusing myoblasts depends on Rols7. ► Mhcl interacts in vitro with Rols7 which is essential for myogenesis. ► Functional redundancy with other myosins is likely as mutants show no muscle defects. ► Mhcl localizes adjacent to Z-discs of sarcomeres and might support muscle integrity.},
doi = {10.1016/J.YEXCR.2012.12.005},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 4,
volume = 319,
place = {United States},
year = {2013},
month = {2}
}