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Title: Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF

Abstract

Migration-stimulating factor (MSF), an oncofetal truncated isoform of fibronectin, is a potent stimulator of cell invasion. However, its distribution and motogenic role in non-small cell lung cancer (NSCLC) have never been identified. In this study, real-time PCR and immunohistochemical staining (IHC) were performed to detect MSF mRNA and protein levels in tumor tissues and matched adjacent tumor-free tissues. Furthermore, to examine the effect of MSF on invasiveness, MSF was upregulated in A549 cells. The invasiveness and viability of A549 cells were then determined using a transwell migration assay and the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assays, respectively. The expression level of MSF in NSCLC tissue was markedly higher than in matched adjacent tumor-free tissue. Additionally, the level of MSF protein expression in stage III and IV NSCLC samples was higher than in stage I and II NSCLC samples. More importantly, we also demonstrated that migration and invasion of A549 cells increased substantially after upregulating MSF, although proliferation remained unchanged. Meanwhile, we found no correlation between increasing motility and invasiveness of MSF-overexpressing cells and expression levels and activities of matrix metalloprotease MMP-2 and MMP-9. Our current study shows that MSF plays a role in migration and invasion of A549 cells and suggestsmore » that MSF may be a potential biomarker of NSCLC progression. - Highlights: • MSF expression was upregulated in NSCLC and correlated with TNM stages. • MSF may be a new biomarker for NSCLC progression. • MSF promoted migration and invasion in A549 cells, independent of MMP-2/MMP-9 expression.« less

Authors:
 [1];  [2];  [3];  [4];  [5]; ;  [1]
  1. Department of Thoracic Surgery, Tangdu Hospital, Forth Military Medical University (China)
  2. (China)
  3. Department of Cardio-thoracic Surgery, Affiliated Hospital of Academy of Military Medical Sciences (China)
  4. Department of Pathology, Affiliated Hospital of Academy of Military Medical Sciences (China)
  5. College of Life Sciences and Bioengineering, Beijing Jiaotong University (China)
Publication Date:
OSTI Identifier:
22278172
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 319; Journal Issue: 17; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MARKERS; LUNGS; MESSENGER-RNA; NEOPLASMS; PROTEINS

Citation Formats

Deng, Xuefeng, E-mail: dengxfdoctor@hotmail.com, Department of Cardio-thoracic Surgery, Affiliated Hospital of Academy of Military Medical Sciences, Ma, Qunfeng, Zhang, Bo, Jiang, Hong, Zhang, Zhipei, and Wang, Yunjie. Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF. United States: N. p., 2013. Web. doi:10.1016/J.YEXCR.2013.05.016.
Deng, Xuefeng, E-mail: dengxfdoctor@hotmail.com, Department of Cardio-thoracic Surgery, Affiliated Hospital of Academy of Military Medical Sciences, Ma, Qunfeng, Zhang, Bo, Jiang, Hong, Zhang, Zhipei, & Wang, Yunjie. Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF. United States. doi:10.1016/J.YEXCR.2013.05.016.
Deng, Xuefeng, E-mail: dengxfdoctor@hotmail.com, Department of Cardio-thoracic Surgery, Affiliated Hospital of Academy of Military Medical Sciences, Ma, Qunfeng, Zhang, Bo, Jiang, Hong, Zhang, Zhipei, and Wang, Yunjie. Tue . "Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF". United States. doi:10.1016/J.YEXCR.2013.05.016.
@article{osti_22278172,
title = {Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF},
author = {Deng, Xuefeng, E-mail: dengxfdoctor@hotmail.com and Department of Cardio-thoracic Surgery, Affiliated Hospital of Academy of Military Medical Sciences and Ma, Qunfeng and Zhang, Bo and Jiang, Hong and Zhang, Zhipei and Wang, Yunjie},
abstractNote = {Migration-stimulating factor (MSF), an oncofetal truncated isoform of fibronectin, is a potent stimulator of cell invasion. However, its distribution and motogenic role in non-small cell lung cancer (NSCLC) have never been identified. In this study, real-time PCR and immunohistochemical staining (IHC) were performed to detect MSF mRNA and protein levels in tumor tissues and matched adjacent tumor-free tissues. Furthermore, to examine the effect of MSF on invasiveness, MSF was upregulated in A549 cells. The invasiveness and viability of A549 cells were then determined using a transwell migration assay and the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assays, respectively. The expression level of MSF in NSCLC tissue was markedly higher than in matched adjacent tumor-free tissue. Additionally, the level of MSF protein expression in stage III and IV NSCLC samples was higher than in stage I and II NSCLC samples. More importantly, we also demonstrated that migration and invasion of A549 cells increased substantially after upregulating MSF, although proliferation remained unchanged. Meanwhile, we found no correlation between increasing motility and invasiveness of MSF-overexpressing cells and expression levels and activities of matrix metalloprotease MMP-2 and MMP-9. Our current study shows that MSF plays a role in migration and invasion of A549 cells and suggests that MSF may be a potential biomarker of NSCLC progression. - Highlights: • MSF expression was upregulated in NSCLC and correlated with TNM stages. • MSF may be a new biomarker for NSCLC progression. • MSF promoted migration and invasion in A549 cells, independent of MMP-2/MMP-9 expression.},
doi = {10.1016/J.YEXCR.2013.05.016},
journal = {Experimental Cell Research},
number = 17,
volume = 319,
place = {United States},
year = {Tue Oct 15 00:00:00 EDT 2013},
month = {Tue Oct 15 00:00:00 EDT 2013}
}