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Title: Nesfatin-1 inhibits ovarian epithelial carcinoma cell proliferation in vitro

Abstract

Highlights: •Nesfatin-1 inhibits the proliferation and growth of HO-8910 cells by G1 phase arrest. •Nesfatin-1 enhances HO-8910 cell apoptosis. •Nesfatin-1 inhibits HO-8910 cell proliferation via mTOR and RhoA/ROCK signaling pathway. •The first report of nesfatin-1-mediated proliferation in ovarian epithelial carcinoma. -- Abstract: Nesfatin-1, an 82-amino-acid peptide derived from a 396-amino-acid precursor protein nucleobindin 2 (NUCB2), was originally identified in hypothalamic nuclei involved in the regulation of food intake. It was recently reported that nesfatin-1 is a novel depot specific adipokine preferentially produced by subcutaneous tissue, with obesity- and food deprivation-regulated expression. Although a relation between ovarian cancer mortality and obesity has been previously established, a role of nesfatin-1 in ovarian epithelial carcinoma remains unknown. The aim of the present study is to examine the effect of nesfatin-1 on ovary carcinoma cells proliferation. We found that nesfatin-1 inhibits the proliferation and growth of HO-8910 cells by G1 phase arrest, this inhibition could be abolished by nesfatin-1 neutralizing antibody. Nesfatin-1 enhances HO-8910 cell apoptosis, activation of mammalian target of rapamycin (mTOR) and RhoA/ROCK signaling pathway block the effects of nesfatin-1-induced apoptosis, therefore reverses the inhibition of HO-8910 cell proliferation by nesfatin-1. In conclusion, the present study demonstrated that nesfatin-1 can inhibit themore » proliferation in human ovarian epithelial carcinoma cell line HO-8910 cells through inducing apoptosis via mTOR and RhoA/ROCK signaling pathway. This study provides a novel regulatory signaling pathway of nesfatin-1-regulated ovarian epithelial carcinoma growth and may contribute to ovarian cancer prevention and therapy, especially in obese patients.« less

Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
22242153
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 440; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACIDS; ANIMAL TISSUES; ANTIBODIES; APOPTOSIS; CARCINOMAS; CELL PROLIFERATION; FOOD; IN VITRO; INHIBITION; INTAKE; METABOLIC DISEASES; MORTALITY; OVARIES; PEPTIDES

Citation Formats

Xu, Yang, Pang, Xiaoyan, Dong, Mei, Wen, Fang, E-mail: wenfang64@hotmail.com, and Zhang, Yi, E-mail: syzi960@yahoo.com. Nesfatin-1 inhibits ovarian epithelial carcinoma cell proliferation in vitro. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.06.001.
Xu, Yang, Pang, Xiaoyan, Dong, Mei, Wen, Fang, E-mail: wenfang64@hotmail.com, & Zhang, Yi, E-mail: syzi960@yahoo.com. Nesfatin-1 inhibits ovarian epithelial carcinoma cell proliferation in vitro. United States. doi:10.1016/J.BBRC.2013.06.001.
Xu, Yang, Pang, Xiaoyan, Dong, Mei, Wen, Fang, E-mail: wenfang64@hotmail.com, and Zhang, Yi, E-mail: syzi960@yahoo.com. 2013. "Nesfatin-1 inhibits ovarian epithelial carcinoma cell proliferation in vitro". United States. doi:10.1016/J.BBRC.2013.06.001.
@article{osti_22242153,
title = {Nesfatin-1 inhibits ovarian epithelial carcinoma cell proliferation in vitro},
author = {Xu, Yang and Pang, Xiaoyan and Dong, Mei and Wen, Fang, E-mail: wenfang64@hotmail.com and Zhang, Yi, E-mail: syzi960@yahoo.com},
abstractNote = {Highlights: •Nesfatin-1 inhibits the proliferation and growth of HO-8910 cells by G1 phase arrest. •Nesfatin-1 enhances HO-8910 cell apoptosis. •Nesfatin-1 inhibits HO-8910 cell proliferation via mTOR and RhoA/ROCK signaling pathway. •The first report of nesfatin-1-mediated proliferation in ovarian epithelial carcinoma. -- Abstract: Nesfatin-1, an 82-amino-acid peptide derived from a 396-amino-acid precursor protein nucleobindin 2 (NUCB2), was originally identified in hypothalamic nuclei involved in the regulation of food intake. It was recently reported that nesfatin-1 is a novel depot specific adipokine preferentially produced by subcutaneous tissue, with obesity- and food deprivation-regulated expression. Although a relation between ovarian cancer mortality and obesity has been previously established, a role of nesfatin-1 in ovarian epithelial carcinoma remains unknown. The aim of the present study is to examine the effect of nesfatin-1 on ovary carcinoma cells proliferation. We found that nesfatin-1 inhibits the proliferation and growth of HO-8910 cells by G1 phase arrest, this inhibition could be abolished by nesfatin-1 neutralizing antibody. Nesfatin-1 enhances HO-8910 cell apoptosis, activation of mammalian target of rapamycin (mTOR) and RhoA/ROCK signaling pathway block the effects of nesfatin-1-induced apoptosis, therefore reverses the inhibition of HO-8910 cell proliferation by nesfatin-1. In conclusion, the present study demonstrated that nesfatin-1 can inhibit the proliferation in human ovarian epithelial carcinoma cell line HO-8910 cells through inducing apoptosis via mTOR and RhoA/ROCK signaling pathway. This study provides a novel regulatory signaling pathway of nesfatin-1-regulated ovarian epithelial carcinoma growth and may contribute to ovarian cancer prevention and therapy, especially in obese patients.},
doi = {10.1016/J.BBRC.2013.06.001},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 440,
place = {United States},
year = 2013,
month =
}
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