skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Characterization of the nuclear localization signal of the mouse TET3 protein

Abstract

Highlights: •Amino acid sequence KKRK is responsible for nuclear localization of TET3. •Amino acid sequence KKRK are capable of targeting the cytoplasmic proteins to the nucleus. •Amino acid sequence KKRK are conserved in TET3 orthologs. -- Abstract: DNA demethylation is associated with gene activation and is mediated by a family of ten-eleven translocation (TET) dioxygenase. The TET3 protein is a 1668-amino-acid DNA demethylase that is predicted to possess five nuclear localization signals (NLSs). In this paper, we used a series of green fluorescent protein-tagged and mutation constructs to identify a conserved NLS (KKRK) embedded between amino acid 1615 and 1618 of mouse TET3. The KKRK sequence facilitates the cytoplasmic protein’s translocation into the nucleus. Additionally TET3 may be imported into the nucleus by importin-α and importin-β.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22242113
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 439; Journal Issue: 3; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACIDS; FLUORESCENCE; GLUTATHIONE; MICE; STH

Citation Formats

Xiao, Peng, Zhou, Xiao-long, Zhang, Hong-xiao, Xiong, Kai, Teng, Yun, Huang, Xian-ju, Cao, Rui, Wang, Yi, and Liu, Hong-lin. Characterization of the nuclear localization signal of the mouse TET3 protein. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.08.075.
Xiao, Peng, Zhou, Xiao-long, Zhang, Hong-xiao, Xiong, Kai, Teng, Yun, Huang, Xian-ju, Cao, Rui, Wang, Yi, & Liu, Hong-lin. Characterization of the nuclear localization signal of the mouse TET3 protein. United States. doi:10.1016/J.BBRC.2013.08.075.
Xiao, Peng, Zhou, Xiao-long, Zhang, Hong-xiao, Xiong, Kai, Teng, Yun, Huang, Xian-ju, Cao, Rui, Wang, Yi, and Liu, Hong-lin. Fri . "Characterization of the nuclear localization signal of the mouse TET3 protein". United States. doi:10.1016/J.BBRC.2013.08.075.
@article{osti_22242113,
title = {Characterization of the nuclear localization signal of the mouse TET3 protein},
author = {Xiao, Peng and Zhou, Xiao-long and Zhang, Hong-xiao and Xiong, Kai and Teng, Yun and Huang, Xian-ju and Cao, Rui and Wang, Yi and Liu, Hong-lin},
abstractNote = {Highlights: •Amino acid sequence KKRK is responsible for nuclear localization of TET3. •Amino acid sequence KKRK are capable of targeting the cytoplasmic proteins to the nucleus. •Amino acid sequence KKRK are conserved in TET3 orthologs. -- Abstract: DNA demethylation is associated with gene activation and is mediated by a family of ten-eleven translocation (TET) dioxygenase. The TET3 protein is a 1668-amino-acid DNA demethylase that is predicted to possess five nuclear localization signals (NLSs). In this paper, we used a series of green fluorescent protein-tagged and mutation constructs to identify a conserved NLS (KKRK) embedded between amino acid 1615 and 1618 of mouse TET3. The KKRK sequence facilitates the cytoplasmic protein’s translocation into the nucleus. Additionally TET3 may be imported into the nucleus by importin-α and importin-β.},
doi = {10.1016/J.BBRC.2013.08.075},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 439,
place = {United States},
year = {2013},
month = {9}
}