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Title: Localization and function of KLF4 in cytoplasm of vascular smooth muscle cell

Abstract

Highlights: •PDGF-BB prompts the translocation of KLF4 to the cytoplasm. •PDGF-BB promotes interaction between KLF4 and actin in the cytoplasm. •Phosphorylation and SUMOylation of KLF4 participates in regulation of cytoskeletal organization. •KLF4 regulates cytoskeleton by promoting the expression of contraction-associated genes. -- Abstract: The Krüppel-like factor 4 is a DNA-binding transcriptional regulator that regulates a diverse array of cellular processes, including development, differentiation, proliferation, and apoptosis. The previous studies about KLF4 functions mainly focused on its role as a transcription factor, its functions in the cytoplasm are still unknown. In this study, we found that PDGF-BB could prompt the translocation of KLF4 to the cytoplasm through CRM1-mediated nuclear export pathway in vascular smooth muscle cells (VSMCs) and increased the interaction of KLF4 with actin in the cytoplasm. Further study showed that both KLF4 phosphorylation and SUMOylation induced by PDGF-BB participates in regulation of cytoskeletal organization by stabilizing the actin cytoskeleton in VSMCs. In conclusion, these results identify that KLF4 participates in the cytoskeletal organization by stabilizing cytoskeleton in the cytoplasm of VSMCs.

Authors:
 [1];  [2]; ; ; ;  [1];  [1]
  1. Department of Biochemistry and Molecular Biology, The Key Laboratory of Neurobiology and Vascular Biology (China)
  2. (China)
Publication Date:
OSTI Identifier:
22239639
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 436; Journal Issue: 2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; APOPTOSIS; CYTOPLASM; DNA; MICROTUBULES; MUSCLES; PHOSPHORYLATION; TRANSCRIPTION FACTORS; TRANSLOCATION

Citation Formats

Liu, Yan, The Third Hospital of Hebei Medical University, Shijazhuang, Zheng, Bin, Zhang, Xin-hua, Nie, Chan-juan, Li, Yong-hui, and Wen, Jin-kun, E-mail: wjk@hebmu.edu.cn. Localization and function of KLF4 in cytoplasm of vascular smooth muscle cell. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.05.067.
Liu, Yan, The Third Hospital of Hebei Medical University, Shijazhuang, Zheng, Bin, Zhang, Xin-hua, Nie, Chan-juan, Li, Yong-hui, & Wen, Jin-kun, E-mail: wjk@hebmu.edu.cn. Localization and function of KLF4 in cytoplasm of vascular smooth muscle cell. United States. doi:10.1016/J.BBRC.2013.05.067.
Liu, Yan, The Third Hospital of Hebei Medical University, Shijazhuang, Zheng, Bin, Zhang, Xin-hua, Nie, Chan-juan, Li, Yong-hui, and Wen, Jin-kun, E-mail: wjk@hebmu.edu.cn. Fri . "Localization and function of KLF4 in cytoplasm of vascular smooth muscle cell". United States. doi:10.1016/J.BBRC.2013.05.067.
@article{osti_22239639,
title = {Localization and function of KLF4 in cytoplasm of vascular smooth muscle cell},
author = {Liu, Yan and The Third Hospital of Hebei Medical University, Shijazhuang and Zheng, Bin and Zhang, Xin-hua and Nie, Chan-juan and Li, Yong-hui and Wen, Jin-kun, E-mail: wjk@hebmu.edu.cn},
abstractNote = {Highlights: •PDGF-BB prompts the translocation of KLF4 to the cytoplasm. •PDGF-BB promotes interaction between KLF4 and actin in the cytoplasm. •Phosphorylation and SUMOylation of KLF4 participates in regulation of cytoskeletal organization. •KLF4 regulates cytoskeleton by promoting the expression of contraction-associated genes. -- Abstract: The Krüppel-like factor 4 is a DNA-binding transcriptional regulator that regulates a diverse array of cellular processes, including development, differentiation, proliferation, and apoptosis. The previous studies about KLF4 functions mainly focused on its role as a transcription factor, its functions in the cytoplasm are still unknown. In this study, we found that PDGF-BB could prompt the translocation of KLF4 to the cytoplasm through CRM1-mediated nuclear export pathway in vascular smooth muscle cells (VSMCs) and increased the interaction of KLF4 with actin in the cytoplasm. Further study showed that both KLF4 phosphorylation and SUMOylation induced by PDGF-BB participates in regulation of cytoskeletal organization by stabilizing the actin cytoskeleton in VSMCs. In conclusion, these results identify that KLF4 participates in the cytoskeletal organization by stabilizing cytoskeleton in the cytoplasm of VSMCs.},
doi = {10.1016/J.BBRC.2013.05.067},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 436,
place = {United States},
year = {Fri Jun 28 00:00:00 EDT 2013},
month = {Fri Jun 28 00:00:00 EDT 2013}
}