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Title: Rice Rab11 is required for JA-mediated defense signaling

Abstract

Highlights: •OsRab11 interacts with OsOPR8. •OsOPR8 is localized in the cytosol and peroxisome. •OsRab11 enhances the NADPH consumption by OsOPR8. •Transgenic Arabidopsis overexpressing OsRab11 represents a pathogen-resistant phenotype. -- Abstract: Rab proteins play an essential role in regulating vesicular transport in eukaryotic cells. Previously, we characterized OsRab11, which in concert with OsGAP1 and OsGDI3 regulates vesicular trafficking from the trans-Golgi network (TGN) to the plasma membrane or vacuole. To further elucidate the physiological function of OsRab11 in plants, we performed yeast two-hybrid screens using OsRab11 as bait. OsOPR8 was isolated and shown to interact with OsRab11. A co-immunoprecipitation assay confirmed this interaction. The green fluorescent protein-OsOPR8 fusion product was targeted to the cytoplasm and peroxisomes of protoplasts from Arabidopsis thaliana. OsOPR8 exhibited NADPH-dependent reduction activity when 2-cyclohexen-1-one (CyHE) and 12-oxo-phytodienoic acid (OPDA) were supplied as possible substrates. Interestingly, NADPH oxidation by OsOPR8 was increased when wild-type OsRab11 or the constitutively active form of OsRab11 (Q78L) were included in the reaction mix, but not when the dominant negative form of OsRab11 (S28N) was included. OsRab11 was expressed broadly in plants and both OsRab11 and OsOPR8 were induced by jasmonic acid (JA) and elicitor treatments. Overexpressed OsRab11 transgenic plants showed resistance tomore » pathogens through induced expression of JA-responsive genes. In conclusion, OsRab11 may be required for JA-mediated defense signaling by activating the reducing activity of OsOPR8.« less

Authors:
 [1];  [2];  [1];  [3];  [4];  [1];  [5];  [3];  [1];  [2]
  1. Department of Molecular Biotechnology, Dong-A University, Busan 604-714 (Korea, Republic of)
  2. (Korea, Republic of)
  3. Division of Applied Life Sciences (BK21), Graduate School of Gyeongsang National University, Jinju 660-701 (Korea, Republic of)
  4. Eco-Friendliness Research Department, Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 660-360 (Korea, Republic of)
  5. Systems and Synthetic Agrobiotech Center, Gyeongsang National University, Jinju 660-701 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22239597
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 434; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ARABIDOPSIS; CYTOPLASM; FLUORESCENCE; HYBRIDIZATION; OXIDATION; PATHOGENS; PHENOTYPE; PROTEINS; RICE; TRANSGENIC PLANTS; YEASTS

Citation Formats

Hong, Min Ji, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, Lee, Yun mi, Son, Young Sim, Im, Chak Han, Yi, Young Byung, Rim, Yeong Gil, Bahk, Jeong Dong, E-mail: jdbahk@gnu.ac.kr, Heo, Jae Bok, E-mail: jbheo72@dau.ac.kr, and BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714. Rice Rab11 is required for JA-mediated defense signaling. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.04.014.
Hong, Min Ji, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, Lee, Yun mi, Son, Young Sim, Im, Chak Han, Yi, Young Byung, Rim, Yeong Gil, Bahk, Jeong Dong, E-mail: jdbahk@gnu.ac.kr, Heo, Jae Bok, E-mail: jbheo72@dau.ac.kr, & BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714. Rice Rab11 is required for JA-mediated defense signaling. United States. doi:10.1016/J.BBRC.2013.04.014.
Hong, Min Ji, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, Lee, Yun mi, Son, Young Sim, Im, Chak Han, Yi, Young Byung, Rim, Yeong Gil, Bahk, Jeong Dong, E-mail: jdbahk@gnu.ac.kr, Heo, Jae Bok, E-mail: jbheo72@dau.ac.kr, and BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714. Fri . "Rice Rab11 is required for JA-mediated defense signaling". United States. doi:10.1016/J.BBRC.2013.04.014.
@article{osti_22239597,
title = {Rice Rab11 is required for JA-mediated defense signaling},
author = {Hong, Min Ji and BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714 and Lee, Yun mi and Son, Young Sim and Im, Chak Han and Yi, Young Byung and Rim, Yeong Gil and Bahk, Jeong Dong, E-mail: jdbahk@gnu.ac.kr and Heo, Jae Bok, E-mail: jbheo72@dau.ac.kr and BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714},
abstractNote = {Highlights: •OsRab11 interacts with OsOPR8. •OsOPR8 is localized in the cytosol and peroxisome. •OsRab11 enhances the NADPH consumption by OsOPR8. •Transgenic Arabidopsis overexpressing OsRab11 represents a pathogen-resistant phenotype. -- Abstract: Rab proteins play an essential role in regulating vesicular transport in eukaryotic cells. Previously, we characterized OsRab11, which in concert with OsGAP1 and OsGDI3 regulates vesicular trafficking from the trans-Golgi network (TGN) to the plasma membrane or vacuole. To further elucidate the physiological function of OsRab11 in plants, we performed yeast two-hybrid screens using OsRab11 as bait. OsOPR8 was isolated and shown to interact with OsRab11. A co-immunoprecipitation assay confirmed this interaction. The green fluorescent protein-OsOPR8 fusion product was targeted to the cytoplasm and peroxisomes of protoplasts from Arabidopsis thaliana. OsOPR8 exhibited NADPH-dependent reduction activity when 2-cyclohexen-1-one (CyHE) and 12-oxo-phytodienoic acid (OPDA) were supplied as possible substrates. Interestingly, NADPH oxidation by OsOPR8 was increased when wild-type OsRab11 or the constitutively active form of OsRab11 (Q78L) were included in the reaction mix, but not when the dominant negative form of OsRab11 (S28N) was included. OsRab11 was expressed broadly in plants and both OsRab11 and OsOPR8 were induced by jasmonic acid (JA) and elicitor treatments. Overexpressed OsRab11 transgenic plants showed resistance to pathogens through induced expression of JA-responsive genes. In conclusion, OsRab11 may be required for JA-mediated defense signaling by activating the reducing activity of OsOPR8.},
doi = {10.1016/J.BBRC.2013.04.014},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 434,
place = {United States},
year = {Fri May 17 00:00:00 EDT 2013},
month = {Fri May 17 00:00:00 EDT 2013}
}