skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells

Abstract

Highlights: •Down-regulation of Wnt10a in dental mesenchymal cells impairs odontogenesis of reassociated tooth germs. •Dspp is down- and up-regulated after Wnt10a-knockdown and overexpression in dental mesenchymal cells. •Down-regulation of Wnt10a inhibits proliferation of dental mesenchymal cells. -- Abstract: The WNT10a mutation has been found in patients with abnormal odontogenesis. In mice, Wnt10a expression is found in the tooth germ, but its role has not yet been elucidated. We aimed to investigate the role of Wnt10a in odontogenesis. Mesenchymal cells of the first mandibular molar germ at the bell stage were isolated, transfected with Wnt10a SiRNA or plasmid, and reassociated with epithelial part of the molar germ. Scrambled SiRNA or empty vector was used in the control group. The reassociated tooth germs were transplanted into mice subrenal capsules. After gene modification, dental mesenchymal cells cultured in vitro were checked for cell proliferation and the expression of Dspp was examined. All 12 reassociated tooth germs in the control group resumed odontogenesis, while only 5 of 12 in the Wnt10a knockdown group developed into teeth. After Wnt10a knockdown, the mesenchymal cells cultured in vitro presented repressed proliferation. Wnt10a knockdown and overexpression led to both down- and up-regulation of Dspp. We conclude that themore » down-regulation of Wnt10a impairs odontogensis and cell proliferation, and that Wnt10a regulates Dspp expression in mesenchymal cells. These findings help to elucidate the mechanism of abnormal tooth development in patients with the WNT10A mutation.« less

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22239592
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 434; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; GENE REGULATION; IN VITRO; MICE; MUTATIONS; PATIENTS; TEETH; TRANSPLANTS

Citation Formats

Liu, Yang, E-mail: Ly10160624@163.com, Han, Dong, E-mail: Donghan@bjmu.edu.cn, Wang, Lei, E-mail: wanglei_dentist@163.com, and Feng, Hailan, E-mail: kqfenghl@bjmu.edu.cn. Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.03.088.
Liu, Yang, E-mail: Ly10160624@163.com, Han, Dong, E-mail: Donghan@bjmu.edu.cn, Wang, Lei, E-mail: wanglei_dentist@163.com, & Feng, Hailan, E-mail: kqfenghl@bjmu.edu.cn. Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells. United States. doi:10.1016/J.BBRC.2013.03.088.
Liu, Yang, E-mail: Ly10160624@163.com, Han, Dong, E-mail: Donghan@bjmu.edu.cn, Wang, Lei, E-mail: wanglei_dentist@163.com, and Feng, Hailan, E-mail: kqfenghl@bjmu.edu.cn. Fri . "Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells". United States. doi:10.1016/J.BBRC.2013.03.088.
@article{osti_22239592,
title = {Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells},
author = {Liu, Yang, E-mail: Ly10160624@163.com and Han, Dong, E-mail: Donghan@bjmu.edu.cn and Wang, Lei, E-mail: wanglei_dentist@163.com and Feng, Hailan, E-mail: kqfenghl@bjmu.edu.cn},
abstractNote = {Highlights: •Down-regulation of Wnt10a in dental mesenchymal cells impairs odontogenesis of reassociated tooth germs. •Dspp is down- and up-regulated after Wnt10a-knockdown and overexpression in dental mesenchymal cells. •Down-regulation of Wnt10a inhibits proliferation of dental mesenchymal cells. -- Abstract: The WNT10a mutation has been found in patients with abnormal odontogenesis. In mice, Wnt10a expression is found in the tooth germ, but its role has not yet been elucidated. We aimed to investigate the role of Wnt10a in odontogenesis. Mesenchymal cells of the first mandibular molar germ at the bell stage were isolated, transfected with Wnt10a SiRNA or plasmid, and reassociated with epithelial part of the molar germ. Scrambled SiRNA or empty vector was used in the control group. The reassociated tooth germs were transplanted into mice subrenal capsules. After gene modification, dental mesenchymal cells cultured in vitro were checked for cell proliferation and the expression of Dspp was examined. All 12 reassociated tooth germs in the control group resumed odontogenesis, while only 5 of 12 in the Wnt10a knockdown group developed into teeth. After Wnt10a knockdown, the mesenchymal cells cultured in vitro presented repressed proliferation. Wnt10a knockdown and overexpression led to both down- and up-regulation of Dspp. We conclude that the down-regulation of Wnt10a impairs odontogensis and cell proliferation, and that Wnt10a regulates Dspp expression in mesenchymal cells. These findings help to elucidate the mechanism of abnormal tooth development in patients with the WNT10A mutation.},
doi = {10.1016/J.BBRC.2013.03.088},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 434,
place = {United States},
year = {Fri May 17 00:00:00 EDT 2013},
month = {Fri May 17 00:00:00 EDT 2013}
}