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Title: Tet1 is required for Rb phosphorylation during G1/S phase transition

Abstract

Highlights: •Tet1 was required for NIT3T3 proliferation. •Tet1 depletion inhibited G1-S entry. •Cyclin D1 accumulation and Rb phosphorylation was blocked by Tet1 knockdown. -- Abstract: DNA methylation plays an important role in many biological processes, including regulation of gene expression, maintenance of chromatin conformation and genomic stability. TET-family proteins convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), which indicates that these enzymes may participate in DNA demethylation. The function of TET1 has not yet been well characterized in somatic cells. Here, we show that depletion of Tet1 in NIH3T3 cells inhibits cell growth. Furthermore, Tet1 knockdown blocks cyclin D1 accumulation in G1 phase, inhibits Rb phosphorylation and consequently delays entrance to G1/S phase. Taken together, this study demonstrates that Tet1 is required for cell proliferation and that this process is mediated through the Rb pathway.

Authors:
 [1]; ; ; ; ; ;  [2]; ;  [1];  [2];  [1]
  1. Department of Urology, Tongji Hospital of Tongji University, Shanghai (China)
  2. State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology and Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai (China)
Publication Date:
OSTI Identifier:
22239569
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 434; Journal Issue: 2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; CHROMATIN; DNA; HYDROXYLASES; METHYLATION; PHASE TRANSFORMATIONS; PHOSPHORYLATION; SOMATIC CELLS

Citation Formats

Huang, Shengsong, Zhu, Ziqi, Wang, Yiqin, Wang, Yanru, Xu, Longxia, Chen, Xuemei, Xu, Qing, Zhang, Qimin, Zhao, Xin, Yu, Yi, E-mail: yuyi@sibcb.ac.cn, and Wu, Denglong, E-mail: wudenglong@163.com. Tet1 is required for Rb phosphorylation during G1/S phase transition. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.02.110.
Huang, Shengsong, Zhu, Ziqi, Wang, Yiqin, Wang, Yanru, Xu, Longxia, Chen, Xuemei, Xu, Qing, Zhang, Qimin, Zhao, Xin, Yu, Yi, E-mail: yuyi@sibcb.ac.cn, & Wu, Denglong, E-mail: wudenglong@163.com. Tet1 is required for Rb phosphorylation during G1/S phase transition. United States. doi:10.1016/J.BBRC.2013.02.110.
Huang, Shengsong, Zhu, Ziqi, Wang, Yiqin, Wang, Yanru, Xu, Longxia, Chen, Xuemei, Xu, Qing, Zhang, Qimin, Zhao, Xin, Yu, Yi, E-mail: yuyi@sibcb.ac.cn, and Wu, Denglong, E-mail: wudenglong@163.com. Fri . "Tet1 is required for Rb phosphorylation during G1/S phase transition". United States. doi:10.1016/J.BBRC.2013.02.110.
@article{osti_22239569,
title = {Tet1 is required for Rb phosphorylation during G1/S phase transition},
author = {Huang, Shengsong and Zhu, Ziqi and Wang, Yiqin and Wang, Yanru and Xu, Longxia and Chen, Xuemei and Xu, Qing and Zhang, Qimin and Zhao, Xin and Yu, Yi, E-mail: yuyi@sibcb.ac.cn and Wu, Denglong, E-mail: wudenglong@163.com},
abstractNote = {Highlights: •Tet1 was required for NIT3T3 proliferation. •Tet1 depletion inhibited G1-S entry. •Cyclin D1 accumulation and Rb phosphorylation was blocked by Tet1 knockdown. -- Abstract: DNA methylation plays an important role in many biological processes, including regulation of gene expression, maintenance of chromatin conformation and genomic stability. TET-family proteins convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), which indicates that these enzymes may participate in DNA demethylation. The function of TET1 has not yet been well characterized in somatic cells. Here, we show that depletion of Tet1 in NIH3T3 cells inhibits cell growth. Furthermore, Tet1 knockdown blocks cyclin D1 accumulation in G1 phase, inhibits Rb phosphorylation and consequently delays entrance to G1/S phase. Taken together, this study demonstrates that Tet1 is required for cell proliferation and that this process is mediated through the Rb pathway.},
doi = {10.1016/J.BBRC.2013.02.110},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 434,
place = {United States},
year = {2013},
month = {5}
}