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Title: Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish

Abstract

Highlights: ► Sema4d was expressed at all developmental stages of zebrafish. ► Knockdown of sema4d in embryos resulted in defects in the hindbrain and the trunk structure. ► Knockdown of sema4d in embryos upregulated the expression of three hindbrain rhombomere markers. ► Knockdown of sema4d in embryos increased the expression of myogenic regulatory factors. ► Knockdown of sema4d in embryos resulted in an obvious increase of cell apoptosis. -- Abstract: Semaphorin4d (SEMA4D), also known as CD100, an oligodendrocyte secreted R-Ras GTPase-activating protein (GAP), affecting axonal growth is involved in a range of processes including cell adhesion, motility, angiogenesis, immune responses and tumour progression. However, its actual physiological mechanisms and its role in development remain unclear. This study has focused on the role of sema4d in the development and expression patterns in zebrafish embryos and the effect of its suppression on development using sema4d-specific antisense morpholino-oligonucleotides. In this study the knockdown of sema4d, expressed at all developmental stages, lead to defects in the hindbrain and trunk structure of zebrafish embryos. In addition, these phenotypes appeared to be associated with the abnormal expression of three hindbrain rhombomere boundary markers, wnt1, epha4a and foxb1.2, and two myogenic regulatory factors, myod and myog. Further,more » a notable increase of cell apoptosis appeared in the sema4d knockdown embryos, while no obvious reduction in cell proliferation was observed. Collectively, these data suggest that sema4d plays an important role in the development of the hindbrain and skeletal muscle.« less

Authors:
; ; ; ; ; ; ; ; ;  [1];  [1]
  1. Cancer Biology Research Centre, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030 (China)
Publication Date:
OSTI Identifier:
22239535
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 433; Journal Issue: 2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANGIOGENESIS; APOPTOSIS; CELL PROLIFERATION; EMBRYOS; INHIBITION; MUSCLES; OLIGONUCLEOTIDES; PHENOTYPE

Citation Formats

Yang, Jie, Zeng, Zhen, Wei, Juncheng, Jiang, Lijun, Ma, Quanfu, Wu, Mingfu, Huang, Xiaoyuan, Ye, Shuangmei, Li, Ye, Ma, Ding, and Gao, Qinglei, E-mail: qlgao@tjh.tjmu.edu.cn. Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.02.085.
Yang, Jie, Zeng, Zhen, Wei, Juncheng, Jiang, Lijun, Ma, Quanfu, Wu, Mingfu, Huang, Xiaoyuan, Ye, Shuangmei, Li, Ye, Ma, Ding, & Gao, Qinglei, E-mail: qlgao@tjh.tjmu.edu.cn. Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish. United States. doi:10.1016/J.BBRC.2013.02.085.
Yang, Jie, Zeng, Zhen, Wei, Juncheng, Jiang, Lijun, Ma, Quanfu, Wu, Mingfu, Huang, Xiaoyuan, Ye, Shuangmei, Li, Ye, Ma, Ding, and Gao, Qinglei, E-mail: qlgao@tjh.tjmu.edu.cn. Fri . "Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish". United States. doi:10.1016/J.BBRC.2013.02.085.
@article{osti_22239535,
title = {Sema4d is required for the development of the hindbrain boundary and skeletal muscle in zebrafish},
author = {Yang, Jie and Zeng, Zhen and Wei, Juncheng and Jiang, Lijun and Ma, Quanfu and Wu, Mingfu and Huang, Xiaoyuan and Ye, Shuangmei and Li, Ye and Ma, Ding and Gao, Qinglei, E-mail: qlgao@tjh.tjmu.edu.cn},
abstractNote = {Highlights: ► Sema4d was expressed at all developmental stages of zebrafish. ► Knockdown of sema4d in embryos resulted in defects in the hindbrain and the trunk structure. ► Knockdown of sema4d in embryos upregulated the expression of three hindbrain rhombomere markers. ► Knockdown of sema4d in embryos increased the expression of myogenic regulatory factors. ► Knockdown of sema4d in embryos resulted in an obvious increase of cell apoptosis. -- Abstract: Semaphorin4d (SEMA4D), also known as CD100, an oligodendrocyte secreted R-Ras GTPase-activating protein (GAP), affecting axonal growth is involved in a range of processes including cell adhesion, motility, angiogenesis, immune responses and tumour progression. However, its actual physiological mechanisms and its role in development remain unclear. This study has focused on the role of sema4d in the development and expression patterns in zebrafish embryos and the effect of its suppression on development using sema4d-specific antisense morpholino-oligonucleotides. In this study the knockdown of sema4d, expressed at all developmental stages, lead to defects in the hindbrain and trunk structure of zebrafish embryos. In addition, these phenotypes appeared to be associated with the abnormal expression of three hindbrain rhombomere boundary markers, wnt1, epha4a and foxb1.2, and two myogenic regulatory factors, myod and myog. Further, a notable increase of cell apoptosis appeared in the sema4d knockdown embryos, while no obvious reduction in cell proliferation was observed. Collectively, these data suggest that sema4d plays an important role in the development of the hindbrain and skeletal muscle.},
doi = {10.1016/J.BBRC.2013.02.085},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 433,
place = {United States},
year = {2013},
month = {4}
}