skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: miR-330 regulates the proliferation of colorectal cancer cells by targeting Cdc42

Abstract

Highlights: ► miR-330 was inversely correlated with Cdc42 in colorectal cancer cells. ► Elevated miR-330 suppressed cell proliferation in vivo and in vitro. ► Elevated miR-330 mimicked the effect of Cdc42 knockdown. ► Restoration of Cdc42 could partially attenuate the effects of miR-330. -- Abstract: MicroRNAs are small non-coding RNA molecules that play important roles in the multistep process of colorectal carcinoma (CRC) development. However, the miRNA–mRNA regulatory network is far from being fully understood. The objective of this study was to investigate the expression and the biological roles of miR-330 in colorectal cancer cells. Cdc42, one of the best characterized members of the Rho GTPase family, was found to be up-regulated in several types of human tumors including CRC and has been implicated in cancer initiation and progression. In the present study, we identified miR-330, as a potential regulator of Cdc42, was found to be inversely correlated with Cdc42 expression in colorectal cancer cell lines. Ectopic expression of miR-330 down-regulated Cdc42 expression at both protein and mRNA level, mimicked the effect of Cdc42 knockdown in inhibiting proliferation, inducing G1 cell cycle arrest and apoptosis of the colorectal cancer cells, whereas restoration of Cdc42 could partially attenuate the effects ofmore » miR-330. In addition, elevated expression of miR-330 could suppress the immediate downstream effectors of Cdc42 and inhibit the growth of colorectal cancer cells in vivo. To sum up, our results establish a role of miR-330 in negatively regulating Cdc42 expression and colorectal cancer cell proliferation. They suggest that manipulating the expression level of Cdc42 by miR-330 has the potential to influence colorectal cancer progression.« less

Authors:
 [1]; ;  [2];  [1];  [1]
  1. The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001 (China)
  2. The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001 (China)
Publication Date:
OSTI Identifier:
22239500
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 431; Journal Issue: 3; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; BIOLOGICAL RECOVERY; CARCINOMAS; CELL CYCLE; CELL PROLIFERATION; IN VITRO; IN VIVO; MESSENGER-RNA; PROTEINS

Citation Formats

Li, Yuefeng, Zhu, Xiaolan, Xu, Wenlin, Wang, Dongqing, and Yan, Jinchuan, E-mail: jiangdalyf2009@126.com. miR-330 regulates the proliferation of colorectal cancer cells by targeting Cdc42. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.01.016.
Li, Yuefeng, Zhu, Xiaolan, Xu, Wenlin, Wang, Dongqing, & Yan, Jinchuan, E-mail: jiangdalyf2009@126.com. miR-330 regulates the proliferation of colorectal cancer cells by targeting Cdc42. United States. doi:10.1016/J.BBRC.2013.01.016.
Li, Yuefeng, Zhu, Xiaolan, Xu, Wenlin, Wang, Dongqing, and Yan, Jinchuan, E-mail: jiangdalyf2009@126.com. Fri . "miR-330 regulates the proliferation of colorectal cancer cells by targeting Cdc42". United States. doi:10.1016/J.BBRC.2013.01.016.
@article{osti_22239500,
title = {miR-330 regulates the proliferation of colorectal cancer cells by targeting Cdc42},
author = {Li, Yuefeng and Zhu, Xiaolan and Xu, Wenlin and Wang, Dongqing and Yan, Jinchuan, E-mail: jiangdalyf2009@126.com},
abstractNote = {Highlights: ► miR-330 was inversely correlated with Cdc42 in colorectal cancer cells. ► Elevated miR-330 suppressed cell proliferation in vivo and in vitro. ► Elevated miR-330 mimicked the effect of Cdc42 knockdown. ► Restoration of Cdc42 could partially attenuate the effects of miR-330. -- Abstract: MicroRNAs are small non-coding RNA molecules that play important roles in the multistep process of colorectal carcinoma (CRC) development. However, the miRNA–mRNA regulatory network is far from being fully understood. The objective of this study was to investigate the expression and the biological roles of miR-330 in colorectal cancer cells. Cdc42, one of the best characterized members of the Rho GTPase family, was found to be up-regulated in several types of human tumors including CRC and has been implicated in cancer initiation and progression. In the present study, we identified miR-330, as a potential regulator of Cdc42, was found to be inversely correlated with Cdc42 expression in colorectal cancer cell lines. Ectopic expression of miR-330 down-regulated Cdc42 expression at both protein and mRNA level, mimicked the effect of Cdc42 knockdown in inhibiting proliferation, inducing G1 cell cycle arrest and apoptosis of the colorectal cancer cells, whereas restoration of Cdc42 could partially attenuate the effects of miR-330. In addition, elevated expression of miR-330 could suppress the immediate downstream effectors of Cdc42 and inhibit the growth of colorectal cancer cells in vivo. To sum up, our results establish a role of miR-330 in negatively regulating Cdc42 expression and colorectal cancer cell proliferation. They suggest that manipulating the expression level of Cdc42 by miR-330 has the potential to influence colorectal cancer progression.},
doi = {10.1016/J.BBRC.2013.01.016},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 431,
place = {United States},
year = {2013},
month = {2}
}