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Title: Reductive nitrosylation of Methanosarcina acetivorans protoglobin: A comparative study

Abstract

Highlights: ► Methanosarcina acetivorans is a strictly anaerobic non-motile methane-producing Archaea. ► M. acetivorans protoglobin binds preferentially O{sub 2} rather than CO. ► Reductive nitrosylation of ferric M. acetivorans protoglobin. ► Nitrosylation of ferrious M. acetivorans protoglobin. ► M. acetivorans protoglobin is a scavenger of RNS and ROS. -- Abstract: Methanosarcina acetivorans is a strictly anaerobic non-motile methane-producing Archaea expressing protoglobin (Pgb) which might either facilitate O{sub 2} detoxification or act as a CO sensor/supplier in methanogenesis. Unusually, M. acetivorans Pgb (MaPgb) binds preferentially O{sub 2} rather than CO and displays anticooperativity in ligand binding. Here, kinetics and/or thermodynamics of ferric and ferrous MaPgb (MaPgb(III) and MaPgb(II), respectively) nitrosylation are reported. Data were obtained between pH 7.2 and 9.5, at 22.0 °C. Addition of NO to MaPgb(III) leads to the transient formation of MaPgb(III)–NO in equilibrium with MaPgb(II)–NO{sup +}. In turn, MaPgb(II)–NO{sup +} is converted to MaPgb(II) by OH{sup −}-based catalysis. Then, MaPgb(II) binds NO very rapidly leading to MaPgb(II)–NO. The rate-limiting step for reductive nitrosylation of MaPgb(III) is represented by the OH{sup −}-mediated reduction of MaPgb(II)–NO{sup +} to MaPgb(II). Present results highlight the potential role of MaPgb in scavenging of reactive nitrogen and oxygen species.

Authors:
 [1];  [2]; ;  [3]; ;  [4];  [5]
  1. Laboratorio Interdipartimentale di Microscopia Elettronica, Università Roma Tre, Via della Vasca Navale 79, I-00146 Roma (Italy)
  2. Dipartimento di Fisica, Università di Genova, I-16146 Genova (Italy)
  3. Dipartimento di Bioscienze, Università di Milano, Via Celoria 26, I-20133 Milano (Italy)
  4. Dipartimento di Scienze Cliniche e Medicina Traslazionale, Università di Roma Tor Vergata, Via Montpellier 1, I-00133 Roma (Italy)
  5. Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp (Belgium)
Publication Date:
OSTI Identifier:
22224324
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 430; Journal Issue: 4; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES; CARBON MONOXIDE; CATALYSIS; DETOXIFICATION; HEMOGLOBIN; LIGANDS; METHANE; MYOGLOBIN; NITRIC OXIDE; PH VALUE; PROPANE; SCAVENGING; THERMODYNAMICS

Citation Formats

Ascenzi, Paolo, Istituto di Biochimica delle Proteine, CNR, Via Pietro Castellino 111, I-80131 Napoli, Pesce, Alessandra, Nardini, Marco, Bolognesi, Martino, Ciaccio, Chiara, Coletta, Massimo, Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici, Piazza Umberto I 1, I-70121 Bari, and Dewilde, Sylvia. Reductive nitrosylation of Methanosarcina acetivorans protoglobin: A comparative study. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2012.11.122.
Ascenzi, Paolo, Istituto di Biochimica delle Proteine, CNR, Via Pietro Castellino 111, I-80131 Napoli, Pesce, Alessandra, Nardini, Marco, Bolognesi, Martino, Ciaccio, Chiara, Coletta, Massimo, Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici, Piazza Umberto I 1, I-70121 Bari, & Dewilde, Sylvia. Reductive nitrosylation of Methanosarcina acetivorans protoglobin: A comparative study. United States. doi:10.1016/J.BBRC.2012.11.122.
Ascenzi, Paolo, Istituto di Biochimica delle Proteine, CNR, Via Pietro Castellino 111, I-80131 Napoli, Pesce, Alessandra, Nardini, Marco, Bolognesi, Martino, Ciaccio, Chiara, Coletta, Massimo, Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici, Piazza Umberto I 1, I-70121 Bari, and Dewilde, Sylvia. Fri . "Reductive nitrosylation of Methanosarcina acetivorans protoglobin: A comparative study". United States. doi:10.1016/J.BBRC.2012.11.122.
@article{osti_22224324,
title = {Reductive nitrosylation of Methanosarcina acetivorans protoglobin: A comparative study},
author = {Ascenzi, Paolo and Istituto di Biochimica delle Proteine, CNR, Via Pietro Castellino 111, I-80131 Napoli and Pesce, Alessandra and Nardini, Marco and Bolognesi, Martino and Ciaccio, Chiara and Coletta, Massimo and Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici, Piazza Umberto I 1, I-70121 Bari and Dewilde, Sylvia},
abstractNote = {Highlights: ► Methanosarcina acetivorans is a strictly anaerobic non-motile methane-producing Archaea. ► M. acetivorans protoglobin binds preferentially O{sub 2} rather than CO. ► Reductive nitrosylation of ferric M. acetivorans protoglobin. ► Nitrosylation of ferrious M. acetivorans protoglobin. ► M. acetivorans protoglobin is a scavenger of RNS and ROS. -- Abstract: Methanosarcina acetivorans is a strictly anaerobic non-motile methane-producing Archaea expressing protoglobin (Pgb) which might either facilitate O{sub 2} detoxification or act as a CO sensor/supplier in methanogenesis. Unusually, M. acetivorans Pgb (MaPgb) binds preferentially O{sub 2} rather than CO and displays anticooperativity in ligand binding. Here, kinetics and/or thermodynamics of ferric and ferrous MaPgb (MaPgb(III) and MaPgb(II), respectively) nitrosylation are reported. Data were obtained between pH 7.2 and 9.5, at 22.0 °C. Addition of NO to MaPgb(III) leads to the transient formation of MaPgb(III)–NO in equilibrium with MaPgb(II)–NO{sup +}. In turn, MaPgb(II)–NO{sup +} is converted to MaPgb(II) by OH{sup −}-based catalysis. Then, MaPgb(II) binds NO very rapidly leading to MaPgb(II)–NO. The rate-limiting step for reductive nitrosylation of MaPgb(III) is represented by the OH{sup −}-mediated reduction of MaPgb(II)–NO{sup +} to MaPgb(II). Present results highlight the potential role of MaPgb in scavenging of reactive nitrogen and oxygen species.},
doi = {10.1016/J.BBRC.2012.11.122},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 430,
place = {United States},
year = {2013},
month = {1}
}