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Title: Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating

Abstract

Advanced biomaterials should also be bioactive with regard to desirable cellular responses, such as selective protein adsorption and cell attachment, proliferation, and differentiation. To enhance cell-material interactions, surface modifications have commonly been performed. Among the various surface modification approaches, atmospheric pressure glow discharge plasma has been used to change a hydrophobic polymer surface to a hydrophilic surface. Poly(L-lactic acid) (PLLA)-derived scaffolds lack cell recognition signals and the hydrophobic nature of PLLA hinders cell seeding. To make PLLA surfaces more conducive to cell attachment and spreading, surface modifications may be used to create cell-biomaterial interfaces that elicit controlled cell adhesion and maintain differentiated phenotypes. In this study, (He) gaseous atmospheric plasma glow discharge was used to change the characteristics of a 3D-type polymeric scaffold from hydrophobic to hydrophilic on both the outer and inner surfaces of the scaffold and the penetration efficiency with fibronectin was investigated. Field-emission scanning electron microscope images showed that some grooves were formed on the PLLA fibers after plasma treatment. X-ray photoelectron spectroscopy data also showed chemical changes in the PLLA structure. After plasma treatment, -CN (285.76 eV) was increased in C1s and -NH{sub 2} (399.70 eV) was increased significantly and –N=CH (400.80 eV) and –NH{sub 3}{supmore » +} (402.05 eV) were newly appeared in N1s. These changes allowed fibronectin to penetrate into the PLLA scaffold; this could be observed by confocal microscopy. In conclusion, helium atmospheric pressure plasma treatment was effective in modifying the polymeric scaffold, making it hydrophilic, and this treatment can also be used in tissue engineering research as needed to make polymers hydrophilic.« less

Authors:
; ; ; ;  [1];  [2];  [3]; ; ;  [4]
  1. Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752 (Korea, Republic of)
  2. (Korea, Republic of)
  3. Department of Industrial Pharmacy, College of Pharmacy, Ewha Womans University, Seoul 120-750 (Korea, Republic of)
  4. Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-Gu, Seoul 120-749 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22218156
Resource Type:
Journal Article
Journal Name:
Journal of Applied Physics
Additional Journal Information:
Journal Volume: 114; Journal Issue: 7; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0021-8979
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ADHESION; ADSORPTION; ANIMAL TISSUES; ATMOSPHERIC PRESSURE; BIOLOGICAL MATERIALS; EV RANGE; FIBERS; FIELD EMISSION; GLOW DISCHARGES; HELIUM; LACTIC ACID; MODIFICATIONS; PLASMA; POLYMERS; PROTEINS; SCANNING ELECTRON MICROSCOPY; SURFACE COATING; SURFACES; X-RAY PHOTOELECTRON SPECTROSCOPY

Citation Formats

Jin Seo, Hyok, Hee Lee, Mi, Kwon, Byeong-Ju, Kim, Hye-Lee, Park, Jong-Chul, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Jin Lee, Seung, Kim, Bong-Jin, Wang, Kang-Kyun, and Kim, Yong-Rok. Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating. United States: N. p., 2013. Web. doi:10.1063/1.4818914.
Jin Seo, Hyok, Hee Lee, Mi, Kwon, Byeong-Ju, Kim, Hye-Lee, Park, Jong-Chul, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Jin Lee, Seung, Kim, Bong-Jin, Wang, Kang-Kyun, & Kim, Yong-Rok. Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating. United States. doi:10.1063/1.4818914.
Jin Seo, Hyok, Hee Lee, Mi, Kwon, Byeong-Ju, Kim, Hye-Lee, Park, Jong-Chul, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Jin Lee, Seung, Kim, Bong-Jin, Wang, Kang-Kyun, and Kim, Yong-Rok. Wed . "Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating". United States. doi:10.1063/1.4818914.
@article{osti_22218156,
title = {Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating},
author = {Jin Seo, Hyok and Hee Lee, Mi and Kwon, Byeong-Ju and Kim, Hye-Lee and Park, Jong-Chul and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752 and Jin Lee, Seung and Kim, Bong-Jin and Wang, Kang-Kyun and Kim, Yong-Rok},
abstractNote = {Advanced biomaterials should also be bioactive with regard to desirable cellular responses, such as selective protein adsorption and cell attachment, proliferation, and differentiation. To enhance cell-material interactions, surface modifications have commonly been performed. Among the various surface modification approaches, atmospheric pressure glow discharge plasma has been used to change a hydrophobic polymer surface to a hydrophilic surface. Poly(L-lactic acid) (PLLA)-derived scaffolds lack cell recognition signals and the hydrophobic nature of PLLA hinders cell seeding. To make PLLA surfaces more conducive to cell attachment and spreading, surface modifications may be used to create cell-biomaterial interfaces that elicit controlled cell adhesion and maintain differentiated phenotypes. In this study, (He) gaseous atmospheric plasma glow discharge was used to change the characteristics of a 3D-type polymeric scaffold from hydrophobic to hydrophilic on both the outer and inner surfaces of the scaffold and the penetration efficiency with fibronectin was investigated. Field-emission scanning electron microscope images showed that some grooves were formed on the PLLA fibers after plasma treatment. X-ray photoelectron spectroscopy data also showed chemical changes in the PLLA structure. After plasma treatment, -CN (285.76 eV) was increased in C1s and -NH{sub 2} (399.70 eV) was increased significantly and –N=CH (400.80 eV) and –NH{sub 3}{sup +} (402.05 eV) were newly appeared in N1s. These changes allowed fibronectin to penetrate into the PLLA scaffold; this could be observed by confocal microscopy. In conclusion, helium atmospheric pressure plasma treatment was effective in modifying the polymeric scaffold, making it hydrophilic, and this treatment can also be used in tissue engineering research as needed to make polymers hydrophilic.},
doi = {10.1063/1.4818914},
journal = {Journal of Applied Physics},
issn = {0021-8979},
number = 7,
volume = 114,
place = {United States},
year = {2013},
month = {8}
}