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Title: The role of DNA methylation in catechol-enhanced erythroid differentiation of K562 cells

Abstract

Catechol is one of phenolic metabolites of benzene in vivo. Catechol is also widely used in pharmaceutical and chemical industries. In addition, fruits, vegetables and cigarette smoke also contain catechol. Our precious study showed that several benzene metabolites (phenol, hydroquinone, and 1,2,4-benzenetriol) inhibited erythroid differentiation of K562 cells. In present study, the effect of catechol on erythroid differentiation of K562 cells was investigated. Moreover, to address the role of DNA methylation in catechol-induced effect on erythroid differentiation in K562 cells, methylation levels of erythroid-specific genes were analyzed by Quantitative MassARRAY methylation analysis platform. Benzidine staining showed that exposure to catechol enhanced hemin-induced hemoglobin accumulation in K562 cells in concentration- and time-dependent manners. The mRNA expression of erythroid specific genes, including α-globin, β-globin, γ-globin, erythroid 5-aminolevulinate synthase, erythroid porphobilinogen deaminase, and transcription factor GATA-1 genes, showed a significant concentration-dependent increase in catechol-treated K562 cells. The exposure to catechol caused a decrease in DNA methylation levels at a few CpG sites in some erythroid specific genes including α-globin, β-globin and erythroid porphobilinogen deaminase genes. These results indicated that catechol improved erythroid differentiation potency of K562 cells at least partly via up-regulating transcription of some erythroid related genes, and suggested that inhibition ofmore » DNA methylation might be involved in up-regulated expression of some erythroid related genes. -- Highlights: ► Catechol enhanced hemin-induced hemoglobin accumulation. ► Exposure to catechol resulted in up-regulated expression of erythroid genes. ► Catechol reduced methylation levels at some CpG sites in erythroid genes.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22215973
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 265; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALKALINE PHOSPHATASE; BENZENE; BENZIDINE; COLONY FORMING UNITS; DESORPTION; DNA; HEME; HEMOGLOBIN; HOMOCYSTEINE; LEAD SULFIDES; LEUKOCYTES; MACROPHAGES; MESSENGER-RNA; METABOLITES; METHIONINE; METHYL TRANSFERASES; METHYLATION; PHENOL; PHOSPHATES; POLYMERASE CHAIN REACTION; PYROCATECHOL; RECEPTORS; RETINOIC ACID; SHRIMP; TIME DEPENDENCE; TOBACCO SMOKES; TRANSCRIPTION FACTORS; VEGETABLES

Citation Formats

Li, Xiao-Fei, Wu, Xiao-Rong, Xue, Ming, Wang, Yan, Wang, Jie, Li, Yang, Suriguga,, Zhang, Guang-Yao, and Yi, Zong-Chun, E-mail: yizc@buaa.edu.cn. The role of DNA methylation in catechol-enhanced erythroid differentiation of K562 cells. United States: N. p., 2012. Web. doi:10.1016/J.TAAP.2012.09.018.
Li, Xiao-Fei, Wu, Xiao-Rong, Xue, Ming, Wang, Yan, Wang, Jie, Li, Yang, Suriguga,, Zhang, Guang-Yao, & Yi, Zong-Chun, E-mail: yizc@buaa.edu.cn. The role of DNA methylation in catechol-enhanced erythroid differentiation of K562 cells. United States. doi:10.1016/J.TAAP.2012.09.018.
Li, Xiao-Fei, Wu, Xiao-Rong, Xue, Ming, Wang, Yan, Wang, Jie, Li, Yang, Suriguga,, Zhang, Guang-Yao, and Yi, Zong-Chun, E-mail: yizc@buaa.edu.cn. Thu . "The role of DNA methylation in catechol-enhanced erythroid differentiation of K562 cells". United States. doi:10.1016/J.TAAP.2012.09.018.
@article{osti_22215973,
title = {The role of DNA methylation in catechol-enhanced erythroid differentiation of K562 cells},
author = {Li, Xiao-Fei and Wu, Xiao-Rong and Xue, Ming and Wang, Yan and Wang, Jie and Li, Yang and Suriguga, and Zhang, Guang-Yao and Yi, Zong-Chun, E-mail: yizc@buaa.edu.cn},
abstractNote = {Catechol is one of phenolic metabolites of benzene in vivo. Catechol is also widely used in pharmaceutical and chemical industries. In addition, fruits, vegetables and cigarette smoke also contain catechol. Our precious study showed that several benzene metabolites (phenol, hydroquinone, and 1,2,4-benzenetriol) inhibited erythroid differentiation of K562 cells. In present study, the effect of catechol on erythroid differentiation of K562 cells was investigated. Moreover, to address the role of DNA methylation in catechol-induced effect on erythroid differentiation in K562 cells, methylation levels of erythroid-specific genes were analyzed by Quantitative MassARRAY methylation analysis platform. Benzidine staining showed that exposure to catechol enhanced hemin-induced hemoglobin accumulation in K562 cells in concentration- and time-dependent manners. The mRNA expression of erythroid specific genes, including α-globin, β-globin, γ-globin, erythroid 5-aminolevulinate synthase, erythroid porphobilinogen deaminase, and transcription factor GATA-1 genes, showed a significant concentration-dependent increase in catechol-treated K562 cells. The exposure to catechol caused a decrease in DNA methylation levels at a few CpG sites in some erythroid specific genes including α-globin, β-globin and erythroid porphobilinogen deaminase genes. These results indicated that catechol improved erythroid differentiation potency of K562 cells at least partly via up-regulating transcription of some erythroid related genes, and suggested that inhibition of DNA methylation might be involved in up-regulated expression of some erythroid related genes. -- Highlights: ► Catechol enhanced hemin-induced hemoglobin accumulation. ► Exposure to catechol resulted in up-regulated expression of erythroid genes. ► Catechol reduced methylation levels at some CpG sites in erythroid genes.},
doi = {10.1016/J.TAAP.2012.09.018},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 265,
place = {United States},
year = {2012},
month = {11}
}