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Title: Study the cytotoxicity of different kinds of water-soluble nanoparticles in human osteoblast-like MG-63 cells

Abstract

Highlights: ► Preparation of three kinds of water-soluble QDs: CdTe, CdTe@SiO{sub 2}, Mn:ZnSe. ► Evaluated the cytotoxicity qualitatively and quantitatively. ► Fluorescent staining. ► Detected the total intracellular cadmium in cells. -- Abstract: Quantum nanoparticles have been applied extensively in biological and medical fields, the cytotoxicity of nanoparticles becomes the key point we should concern. In this paper, the cytotoxicity of three kinds of water-soluble nanoparticles: CdTe, CdTe@SiO{sub 2} and Mn:ZnSe was studied. We evaluated the nanoparticles toxicity qualitatively by observing the morphological changes of human osteoblast-like MG-63 cells at different incubation times and colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out to detect the cell viability quantitatively. The results showed that CdTe nanoparticles with high concentrations caused cells to die largely while CdTe@SiO{sub 2} and Mn:ZnSe nanoparticles had no obvious effect. For further study, we studied the relation between the cell viability and the total cadmium concentration in cells and found that the viability of cells treated with CdTe@SiO{sub 2} nanoparticles was higher than that treated with CdTe nanoparticles. We also discovered that the death rate of cells co-incubated with CdTe nanoparticles was proportional to the total intracellular cadmium concentrations.

Authors:
 [1]; ;  [2];  [1];  [1]
  1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012 (China)
  2. Department of Pathophysiology, Prostate Diseases Prevention and Treatment Research Centre, Norman Bethune Medical School, Jilin University, Changchun 130012 (China)
Publication Date:
OSTI Identifier:
22215605
Resource Type:
Journal Article
Journal Name:
Materials Research Bulletin
Additional Journal Information:
Journal Volume: 47; Journal Issue: 11; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0025-5408
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY; CADMIUM TELLURIDES; CONNECTIVE TISSUE CELLS; ELECTRON MICROSCOPY; FLUORESCENCE; MICROSTRUCTURE; NANOSTRUCTURES; SILICA; SILICON OXIDES; TETRAZOLIUM; TOXICITY; ZINC SELENIDES

Citation Formats

Niu, Lu, Li, Yang, Li, Xiaojie, Gao, Xue, and Su, Xingguang, E-mail: suxg@jlu.edu.cn. Study the cytotoxicity of different kinds of water-soluble nanoparticles in human osteoblast-like MG-63 cells. United States: N. p., 2012. Web. doi:10.1016/J.MATERRESBULL.2012.06.045.
Niu, Lu, Li, Yang, Li, Xiaojie, Gao, Xue, & Su, Xingguang, E-mail: suxg@jlu.edu.cn. Study the cytotoxicity of different kinds of water-soluble nanoparticles in human osteoblast-like MG-63 cells. United States. doi:10.1016/J.MATERRESBULL.2012.06.045.
Niu, Lu, Li, Yang, Li, Xiaojie, Gao, Xue, and Su, Xingguang, E-mail: suxg@jlu.edu.cn. Thu . "Study the cytotoxicity of different kinds of water-soluble nanoparticles in human osteoblast-like MG-63 cells". United States. doi:10.1016/J.MATERRESBULL.2012.06.045.
@article{osti_22215605,
title = {Study the cytotoxicity of different kinds of water-soluble nanoparticles in human osteoblast-like MG-63 cells},
author = {Niu, Lu and Li, Yang and Li, Xiaojie and Gao, Xue and Su, Xingguang, E-mail: suxg@jlu.edu.cn},
abstractNote = {Highlights: ► Preparation of three kinds of water-soluble QDs: CdTe, CdTe@SiO{sub 2}, Mn:ZnSe. ► Evaluated the cytotoxicity qualitatively and quantitatively. ► Fluorescent staining. ► Detected the total intracellular cadmium in cells. -- Abstract: Quantum nanoparticles have been applied extensively in biological and medical fields, the cytotoxicity of nanoparticles becomes the key point we should concern. In this paper, the cytotoxicity of three kinds of water-soluble nanoparticles: CdTe, CdTe@SiO{sub 2} and Mn:ZnSe was studied. We evaluated the nanoparticles toxicity qualitatively by observing the morphological changes of human osteoblast-like MG-63 cells at different incubation times and colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out to detect the cell viability quantitatively. The results showed that CdTe nanoparticles with high concentrations caused cells to die largely while CdTe@SiO{sub 2} and Mn:ZnSe nanoparticles had no obvious effect. For further study, we studied the relation between the cell viability and the total cadmium concentration in cells and found that the viability of cells treated with CdTe@SiO{sub 2} nanoparticles was higher than that treated with CdTe nanoparticles. We also discovered that the death rate of cells co-incubated with CdTe nanoparticles was proportional to the total intracellular cadmium concentrations.},
doi = {10.1016/J.MATERRESBULL.2012.06.045},
journal = {Materials Research Bulletin},
issn = {0025-5408},
number = 11,
volume = 47,
place = {United States},
year = {2012},
month = {11}
}