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Title: Suppression of dendritic cells' maturation and functions by daidzein, a phytoestrogen

Abstract

Isoflavones are ubiquitous compounds in foods and in the environment in general. Daidzein and genistein, the best known of isoflavones, are structurally similar to 17{beta}-estradiol and known to exert estrogenic effects. They also evidence a broad variety of biological properties, including antioxidant, anti-carcinogenic, anti-atherogenic and anti-osteoporotic activities. Previously, daidzein was reported to increase the phagocytic activity of peritoneal macrophages and splenocyte proliferation, and to inhibit nitric oxide (NO) production in macrophages. However, its potential impacts on immune response in dendritic cells (DCs), antigen-presenting cells that link innate and adaptive immunity, have yet to be clearly elucidated. In this study, we evaluated the effects of isoflavones on the maturation and activation of DCs. Isoflavones (formononetin, daidzein, equol, biochanin A, genistein) were found to differentially affect the expression of CD86, a costimulatory molecule, on lipopolysaccharide (LPS)-stimulated DCs. In particular, daidzein significantly and dose-dependently inhibited the expression levels of maturation-associated cell surface markers including CD40, costimulatory molecules (CD80, CD86), and major histocompatibility complex class II (I-A{sup b}) molecule on LPS-stimulated DCs. Daidzein also suppressed pro-inflammatory cytokine production such as IL-12p40, IL-6 and TNF-{alpha}, whereas it didn't affect IL-10 and IL-1{beta} expression. Furthermore, daidzein enhanced endocytosis and inhibited the allo-stimulatory ability of LPS-stimulated DCsmore » on T cells, indicating that daidzein treatment can inhibit the functional maturation of DCs. These results demonstrate that daidzein may exhibit immunosuppressive activity by inhibiting the maturation and activation of DCs. -- Highlights: Black-Right-Pointing-Pointer Daidzein inhibited expression of maturation-associated cell surface markers in DCs. Black-Right-Pointing-Pointer Daidzein suppressed expression of pro-inflammatory cytokines in LPS-stimulated DCs. Black-Right-Pointing-Pointer Daidzein enhanced endocytosis and inhibited allo-stimulatory ability of DCs. Black-Right-Pointing-Pointer Daidzein exhibited immunosuppressive activity by inhibiting the activation of DCs.« less

Authors:
;  [1];  [2]
  1. School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)
  2. Department of Life Science, Sookmyung Women's University, Seoul 140-742 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22212563
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 257; Journal Issue: 2; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIOXIDANTS; BONE MARROW; CARCINOGENS; DENDRITES; ESTRADIOL; FLUORESCENCE; HISTOCOMPATIBILITY COMPLEX; IMMUNOSUPPRESSION; INFLAMMATION; LIPOPROTEINS; LYMPHOCYTES; LYMPHOKINES; MACROPHAGES; NITRIC OXIDE; PROSTAGLANDINS

Citation Formats

Yum, Min Kyu, Jung, Mi Young, Cho, Daeho, and Kim, Tae Sung, E-mail: tskim@korea.ac.kr. Suppression of dendritic cells' maturation and functions by daidzein, a phytoestrogen. United States: N. p., 2011. Web. doi:10.1016/J.TAAP.2011.09.002.
Yum, Min Kyu, Jung, Mi Young, Cho, Daeho, & Kim, Tae Sung, E-mail: tskim@korea.ac.kr. Suppression of dendritic cells' maturation and functions by daidzein, a phytoestrogen. United States. https://doi.org/10.1016/J.TAAP.2011.09.002
Yum, Min Kyu, Jung, Mi Young, Cho, Daeho, and Kim, Tae Sung, E-mail: tskim@korea.ac.kr. Thu . "Suppression of dendritic cells' maturation and functions by daidzein, a phytoestrogen". United States. https://doi.org/10.1016/J.TAAP.2011.09.002.
@article{osti_22212563,
title = {Suppression of dendritic cells' maturation and functions by daidzein, a phytoestrogen},
author = {Yum, Min Kyu and Jung, Mi Young and Cho, Daeho and Kim, Tae Sung, E-mail: tskim@korea.ac.kr},
abstractNote = {Isoflavones are ubiquitous compounds in foods and in the environment in general. Daidzein and genistein, the best known of isoflavones, are structurally similar to 17{beta}-estradiol and known to exert estrogenic effects. They also evidence a broad variety of biological properties, including antioxidant, anti-carcinogenic, anti-atherogenic and anti-osteoporotic activities. Previously, daidzein was reported to increase the phagocytic activity of peritoneal macrophages and splenocyte proliferation, and to inhibit nitric oxide (NO) production in macrophages. However, its potential impacts on immune response in dendritic cells (DCs), antigen-presenting cells that link innate and adaptive immunity, have yet to be clearly elucidated. In this study, we evaluated the effects of isoflavones on the maturation and activation of DCs. Isoflavones (formononetin, daidzein, equol, biochanin A, genistein) were found to differentially affect the expression of CD86, a costimulatory molecule, on lipopolysaccharide (LPS)-stimulated DCs. In particular, daidzein significantly and dose-dependently inhibited the expression levels of maturation-associated cell surface markers including CD40, costimulatory molecules (CD80, CD86), and major histocompatibility complex class II (I-A{sup b}) molecule on LPS-stimulated DCs. Daidzein also suppressed pro-inflammatory cytokine production such as IL-12p40, IL-6 and TNF-{alpha}, whereas it didn't affect IL-10 and IL-1{beta} expression. Furthermore, daidzein enhanced endocytosis and inhibited the allo-stimulatory ability of LPS-stimulated DCs on T cells, indicating that daidzein treatment can inhibit the functional maturation of DCs. These results demonstrate that daidzein may exhibit immunosuppressive activity by inhibiting the maturation and activation of DCs. -- Highlights: Black-Right-Pointing-Pointer Daidzein inhibited expression of maturation-associated cell surface markers in DCs. Black-Right-Pointing-Pointer Daidzein suppressed expression of pro-inflammatory cytokines in LPS-stimulated DCs. Black-Right-Pointing-Pointer Daidzein enhanced endocytosis and inhibited allo-stimulatory ability of DCs. Black-Right-Pointing-Pointer Daidzein exhibited immunosuppressive activity by inhibiting the activation of DCs.},
doi = {10.1016/J.TAAP.2011.09.002},
url = {https://www.osti.gov/biblio/22212563}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 2,
volume = 257,
place = {United States},
year = {2011},
month = {12}
}