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Title: LAMMER kinase Kic1 is involved in pre-mRNA processing

Abstract

The LAMMER kinases are conserved through evolution. They play vital roles in cell growth/differentiation, development, and metabolism. One of the best known functions of the kinases in animal cells is the regulation of pre-mRNA splicing. Kic1 is the LAMMER kinase in fission yeast Schizosaccharomyces pombe. Despite the reported pleiotropic effects of kic1{sup +} deletion/overexpression on various cellular processes the involvement of Kic1 in splicing remains elusive. In this study, we demonstrate for the first time that Kic1 not only is required for efficient splicing but also affects mRNA export, providing evidence for the conserved roles of LAMMER kinases in the unicellular context of fission yeast. Consistent with the hypothesis of its direct participation in multiple steps of pre-mRNA processing, Kic1 is predominantly present in the nucleus during interphase. In addition, the kinase activity of Kic1 plays a role in modulating its own cellular partitioning. Interestingly, Kic1 expression oscillates in a cell cycle-dependent manner and the peak level coincides with mitosis and cytokinesis, revealing a potential mechanism for controlling the kinase activity during the cell cycle. The novel information about the in vivo functions and regulation of Kic1 offers insights into the conserved biological roles fundamental to LAMMER kinases in eukaryotes.

Authors:
 [1]; ; ; ; ;  [1]; ;  [2]
  1. W.M. Keck Science Center, The Claremont Colleges, Claremont, CA 91711 (United States)
  2. Genome Damage and Stability Centre, University of Sussex, Falmer, BN1 9RQ (United Kingdom)
Publication Date:
OSTI Identifier:
22212173
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 317; Journal Issue: 16; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL CELLS; CELL CYCLE; IN VIVO; MESSENGER-RNA; METABOLISM; MITOSIS; PHOSPHORYLATION; PHOSPHOTRANSFERASES; SPLICING; YEASTS

Citation Formats

Tang, Zhaohua, E-mail: ztang@jsd.claremont.edu, Luca, Maria, Portillio, Jessica, Ngo, Benson, Chang, Cathey, Wen, Teresa, Murray, Johanne, and Carr, Antony. LAMMER kinase Kic1 is involved in pre-mRNA processing. United States: N. p., 2011. Web. doi:10.1016/J.YEXCR.2011.06.014.
Tang, Zhaohua, E-mail: ztang@jsd.claremont.edu, Luca, Maria, Portillio, Jessica, Ngo, Benson, Chang, Cathey, Wen, Teresa, Murray, Johanne, & Carr, Antony. LAMMER kinase Kic1 is involved in pre-mRNA processing. United States. doi:10.1016/J.YEXCR.2011.06.014.
Tang, Zhaohua, E-mail: ztang@jsd.claremont.edu, Luca, Maria, Portillio, Jessica, Ngo, Benson, Chang, Cathey, Wen, Teresa, Murray, Johanne, and Carr, Antony. Sat . "LAMMER kinase Kic1 is involved in pre-mRNA processing". United States. doi:10.1016/J.YEXCR.2011.06.014.
@article{osti_22212173,
title = {LAMMER kinase Kic1 is involved in pre-mRNA processing},
author = {Tang, Zhaohua, E-mail: ztang@jsd.claremont.edu and Luca, Maria and Portillio, Jessica and Ngo, Benson and Chang, Cathey and Wen, Teresa and Murray, Johanne and Carr, Antony},
abstractNote = {The LAMMER kinases are conserved through evolution. They play vital roles in cell growth/differentiation, development, and metabolism. One of the best known functions of the kinases in animal cells is the regulation of pre-mRNA splicing. Kic1 is the LAMMER kinase in fission yeast Schizosaccharomyces pombe. Despite the reported pleiotropic effects of kic1{sup +} deletion/overexpression on various cellular processes the involvement of Kic1 in splicing remains elusive. In this study, we demonstrate for the first time that Kic1 not only is required for efficient splicing but also affects mRNA export, providing evidence for the conserved roles of LAMMER kinases in the unicellular context of fission yeast. Consistent with the hypothesis of its direct participation in multiple steps of pre-mRNA processing, Kic1 is predominantly present in the nucleus during interphase. In addition, the kinase activity of Kic1 plays a role in modulating its own cellular partitioning. Interestingly, Kic1 expression oscillates in a cell cycle-dependent manner and the peak level coincides with mitosis and cytokinesis, revealing a potential mechanism for controlling the kinase activity during the cell cycle. The novel information about the in vivo functions and regulation of Kic1 offers insights into the conserved biological roles fundamental to LAMMER kinases in eukaryotes.},
doi = {10.1016/J.YEXCR.2011.06.014},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 16,
volume = 317,
place = {United States},
year = {2011},
month = {10}
}