Nuclear translocation of the 1,25D{sub 3}-MARRS (membrane associated rapid response to steroids) receptor protein and NF{kappa}B in differentiating NB4 leukemia cells
Abstract
1,25 Dihydroxyvitamin D{sub 3} (1,25D{sub 3}) primes NB4 promyelocytic leukemia cells to differentiate along the monocyte/macrophage lineage through a non-genomic mechanism. Here we show that NB4 cells express high levels of the recently identified membrane receptor for 1,25D{sub 3}, which is a distinct gene product from the classical nuclear vitamin D receptor. This 57 kDa protein, named 1,25D{sub 3}-MARRS (Membrane Activated Rapid Response to Steroids)/ERp57/PIA3 appears to associate in a complex with the transcription factor, nuclear factor kappa B (NF{kappa}B). In unstimulated cells, 1,25D{sub 3}-MARRS can be co-immunoprecipitated with antibodies directed at NF{kappa}B, and NF{kappa}B is co-precipitated when antibodies against 1,25D{sub 3}-MARRS or ERp57 are used. Confocal microscopy and subcellular fractionation studies demonstrate that both 1,25D{sub 3}-MARRS and NF{kappa}B begin translocating to the nucleus within minutes of co-stimulation with 1,25D{sub 3} and phorbol ester. The predominant nuclear localization of both proteins precedes the expression of the monocyte/macrophage phenotype and suggests that this event may be critical to the differentiation pathway. This suggests a role for 1,25D{sub 3}-MARRS in the nucleus as a regulator of gene expression. Here it may also regulate the activity of NF{kappa}B and other factors with which it may be interacting.
- Authors:
-
- Departments of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G2W1 (Canada)
- Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G2W1 (Canada)
- Department of Biological Sciences, University of Delaware, Newark, DE 19716 (United States)
- Department of Nutrition and Food Sciences, Center for Integrated BioSystems, Utah State University, Logan, UT 84322 8700 (United States)
- Publication Date:
- OSTI Identifier:
- 22212064
- Resource Type:
- Journal Article
- Journal Name:
- Experimental Cell Research
- Additional Journal Information:
- Journal Volume: 316; Journal Issue: 7; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ANTIBODIES; FRACTIONATION; GENES; HORMONES; LEUKEMIA; MACROPHAGES; MICROSCOPY; MONOCYTES; PHENOTYPE; PHORBOL ESTERS; RECEPTORS; STEROIDS; TRANSCRIPTION FACTORS; TRANSLOCATION; VITAMIN D
Citation Formats
Wu, Wenqing, Beilhartz, Greg, Roy, Yvette, Richard, Cynthia L., Curtin, Maureen, Brown, Lauren, Cadieux, Danielle, Coppolino, Marc, Farach-Carson, Mary C., Nemere, Ilka, and Meckling, Kelly A., E-mail: kmecklin@uoguelph.ca. Nuclear translocation of the 1,25D{sub 3}-MARRS (membrane associated rapid response to steroids) receptor protein and NF{kappa}B in differentiating NB4 leukemia cells. United States: N. p., 2010.
Web. doi:10.1016/J.YEXCR.2010.01.010.
Wu, Wenqing, Beilhartz, Greg, Roy, Yvette, Richard, Cynthia L., Curtin, Maureen, Brown, Lauren, Cadieux, Danielle, Coppolino, Marc, Farach-Carson, Mary C., Nemere, Ilka, & Meckling, Kelly A., E-mail: kmecklin@uoguelph.ca. Nuclear translocation of the 1,25D{sub 3}-MARRS (membrane associated rapid response to steroids) receptor protein and NF{kappa}B in differentiating NB4 leukemia cells. United States. https://doi.org/10.1016/J.YEXCR.2010.01.010
Wu, Wenqing, Beilhartz, Greg, Roy, Yvette, Richard, Cynthia L., Curtin, Maureen, Brown, Lauren, Cadieux, Danielle, Coppolino, Marc, Farach-Carson, Mary C., Nemere, Ilka, and Meckling, Kelly A., E-mail: kmecklin@uoguelph.ca. 2010.
"Nuclear translocation of the 1,25D{sub 3}-MARRS (membrane associated rapid response to steroids) receptor protein and NF{kappa}B in differentiating NB4 leukemia cells". United States. https://doi.org/10.1016/J.YEXCR.2010.01.010.
@article{osti_22212064,
title = {Nuclear translocation of the 1,25D{sub 3}-MARRS (membrane associated rapid response to steroids) receptor protein and NF{kappa}B in differentiating NB4 leukemia cells},
author = {Wu, Wenqing and Beilhartz, Greg and Roy, Yvette and Richard, Cynthia L. and Curtin, Maureen and Brown, Lauren and Cadieux, Danielle and Coppolino, Marc and Farach-Carson, Mary C. and Nemere, Ilka and Meckling, Kelly A., E-mail: kmecklin@uoguelph.ca},
abstractNote = {1,25 Dihydroxyvitamin D{sub 3} (1,25D{sub 3}) primes NB4 promyelocytic leukemia cells to differentiate along the monocyte/macrophage lineage through a non-genomic mechanism. Here we show that NB4 cells express high levels of the recently identified membrane receptor for 1,25D{sub 3}, which is a distinct gene product from the classical nuclear vitamin D receptor. This 57 kDa protein, named 1,25D{sub 3}-MARRS (Membrane Activated Rapid Response to Steroids)/ERp57/PIA3 appears to associate in a complex with the transcription factor, nuclear factor kappa B (NF{kappa}B). In unstimulated cells, 1,25D{sub 3}-MARRS can be co-immunoprecipitated with antibodies directed at NF{kappa}B, and NF{kappa}B is co-precipitated when antibodies against 1,25D{sub 3}-MARRS or ERp57 are used. Confocal microscopy and subcellular fractionation studies demonstrate that both 1,25D{sub 3}-MARRS and NF{kappa}B begin translocating to the nucleus within minutes of co-stimulation with 1,25D{sub 3} and phorbol ester. The predominant nuclear localization of both proteins precedes the expression of the monocyte/macrophage phenotype and suggests that this event may be critical to the differentiation pathway. This suggests a role for 1,25D{sub 3}-MARRS in the nucleus as a regulator of gene expression. Here it may also regulate the activity of NF{kappa}B and other factors with which it may be interacting.},
doi = {10.1016/J.YEXCR.2010.01.010},
url = {https://www.osti.gov/biblio/22212064},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 7,
volume = 316,
place = {United States},
year = {Thu Apr 15 00:00:00 EDT 2010},
month = {Thu Apr 15 00:00:00 EDT 2010}
}