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Title: Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

Abstract

Highlights: Black-Right-Pointing-Pointer MMP-1 is a member of the zinc-dependent endopeptidase family. Black-Right-Pointing-Pointer MMP-1 has no cytotoxic effects on BMSCs. Black-Right-Pointing-Pointer MMP-1 can promote the myogenic differentiation of BMSCs. Black-Right-Pointing-Pointer MyoD and desmin were chosen as myogenic markers in this study. -- Abstract: Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.

Authors:
 [1];  [2]; ; ;  [1];  [3];  [1]
  1. Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhongshan 2nd Road, Guangzhou 510080, Guangdong Province (China)
  2. Department of Rehabilitation, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhongshan 2nd Road, Guangzhou 510080, Guangdong Province (China)
  3. Institute of Molecular Medicine and Genetics, Georgia Health Sciences University, Augusta, GA 30912 (United States)
Publication Date:
OSTI Identifier:
22210337
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 428; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALIZARIN; ANTIGENS; BONE MARROW; CATTLE; CELL PROLIFERATION; ELECTROPHORESIS; IN VITRO; MESSENGER-RNA; MYOBLASTS; PEROXIDASES; POLYMERASE CHAIN REACTION; RADISHES; STEM CELLS

Citation Formats

Zheng, Zhenyang, Leng, Yan, Zhou, Chen, Ma, Zhenyu, Zhong, Zhigang, Shi, Xing-Ming, and Zhang, Weixi, E-mail: weixizhang@qq.com. Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.10.053.
Zheng, Zhenyang, Leng, Yan, Zhou, Chen, Ma, Zhenyu, Zhong, Zhigang, Shi, Xing-Ming, & Zhang, Weixi, E-mail: weixizhang@qq.com. Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro. United States. doi:10.1016/J.BBRC.2012.10.053.
Zheng, Zhenyang, Leng, Yan, Zhou, Chen, Ma, Zhenyu, Zhong, Zhigang, Shi, Xing-Ming, and Zhang, Weixi, E-mail: weixizhang@qq.com. Fri . "Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro". United States. doi:10.1016/J.BBRC.2012.10.053.
@article{osti_22210337,
title = {Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro},
author = {Zheng, Zhenyang and Leng, Yan and Zhou, Chen and Ma, Zhenyu and Zhong, Zhigang and Shi, Xing-Ming and Zhang, Weixi, E-mail: weixizhang@qq.com},
abstractNote = {Highlights: Black-Right-Pointing-Pointer MMP-1 is a member of the zinc-dependent endopeptidase family. Black-Right-Pointing-Pointer MMP-1 has no cytotoxic effects on BMSCs. Black-Right-Pointing-Pointer MMP-1 can promote the myogenic differentiation of BMSCs. Black-Right-Pointing-Pointer MyoD and desmin were chosen as myogenic markers in this study. -- Abstract: Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.},
doi = {10.1016/J.BBRC.2012.10.053},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 428,
place = {United States},
year = {2012},
month = {11}
}