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Title: Stochastic differentiation into an osteoclast lineage from cloned macrophage-like cells

Abstract

Highlights: Black-Right-Pointing-Pointer The frequency of C7 differentiation into osteoclast was low and constant. Black-Right-Pointing-Pointer Only extended C7 cell cultures exponentially increased osteoclast+ cultures. Black-Right-Pointing-Pointer C7 cell differentiation into committed osteoclast precursors is on 'autopilot'. Black-Right-Pointing-Pointer The system may maintain the stem cell self-renewal and differentiation. -- Abstract: Differentiation into osteoclasts is induced by a macrophage colony-stimulating factor and receptor activator of nuclear-factor {kappa}B ligand. The macrophage-like cell line, C7 has the potential to differentiate into osteoclasts when it is cultured with both factors for 6 days. Although C7 is an established cell line, the frequency of differentiation into this lineage was less than 10%, and the ratio was maintained at a constant level, even after repeated cloning. In this study, to increase the differentiation of C7 cells to osteoclasts, C7 derivative treatments with several activators and/or inhibitors were performed for 3 days prior to setting osteoclast induction analysis; however, a reagent to significantly up-regulate the frequency of differentiation was not found. Only extended cultures for osteoclastogenesis exponentially increased the frequency of osteoclast precursors. It is likely that C7 cell differentiation into committed osteoclast precursors is on 'autopilot' rather than requiring specific signals to drive this process.

Authors:
 [1]; ; ; ;  [1];  [2];  [1]
  1. Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-Cho, Yonago, Tottori 683-8503 (Japan)
  2. Planning and Development, Bioindustry Division, Oriental Yeast Co., Ltd, Itabashi-Ku, Tokyo 174-8505 (Japan)
Publication Date:
OSTI Identifier:
22210336
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 428; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACID PHOSPHATASE; BONE MARROW; CELL CULTURES; CELL DIFFERENTIATION; CLONING; LIGANDS; MACROPHAGES; MONOCLONAL ANTIBODIES; PRECURSOR; RECEPTORS; STEM CELLS; STOCHASTIC PROCESSES

Citation Formats

Hayashi, Shin-Ichi, E-mail: shayashi@med.tottori-u.ac.jp, Murata, Akihiko, Okuyama, Kazuki, Shimoda, Yuhki, Hikosaka, Mari, Yasuda, Hisataka, and Yoshino, Miya. Stochastic differentiation into an osteoclast lineage from cloned macrophage-like cells. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.10.052.
Hayashi, Shin-Ichi, E-mail: shayashi@med.tottori-u.ac.jp, Murata, Akihiko, Okuyama, Kazuki, Shimoda, Yuhki, Hikosaka, Mari, Yasuda, Hisataka, & Yoshino, Miya. Stochastic differentiation into an osteoclast lineage from cloned macrophage-like cells. United States. doi:10.1016/J.BBRC.2012.10.052.
Hayashi, Shin-Ichi, E-mail: shayashi@med.tottori-u.ac.jp, Murata, Akihiko, Okuyama, Kazuki, Shimoda, Yuhki, Hikosaka, Mari, Yasuda, Hisataka, and Yoshino, Miya. Fri . "Stochastic differentiation into an osteoclast lineage from cloned macrophage-like cells". United States. doi:10.1016/J.BBRC.2012.10.052.
@article{osti_22210336,
title = {Stochastic differentiation into an osteoclast lineage from cloned macrophage-like cells},
author = {Hayashi, Shin-Ichi, E-mail: shayashi@med.tottori-u.ac.jp and Murata, Akihiko and Okuyama, Kazuki and Shimoda, Yuhki and Hikosaka, Mari and Yasuda, Hisataka and Yoshino, Miya},
abstractNote = {Highlights: Black-Right-Pointing-Pointer The frequency of C7 differentiation into osteoclast was low and constant. Black-Right-Pointing-Pointer Only extended C7 cell cultures exponentially increased osteoclast+ cultures. Black-Right-Pointing-Pointer C7 cell differentiation into committed osteoclast precursors is on 'autopilot'. Black-Right-Pointing-Pointer The system may maintain the stem cell self-renewal and differentiation. -- Abstract: Differentiation into osteoclasts is induced by a macrophage colony-stimulating factor and receptor activator of nuclear-factor {kappa}B ligand. The macrophage-like cell line, C7 has the potential to differentiate into osteoclasts when it is cultured with both factors for 6 days. Although C7 is an established cell line, the frequency of differentiation into this lineage was less than 10%, and the ratio was maintained at a constant level, even after repeated cloning. In this study, to increase the differentiation of C7 cells to osteoclasts, C7 derivative treatments with several activators and/or inhibitors were performed for 3 days prior to setting osteoclast induction analysis; however, a reagent to significantly up-regulate the frequency of differentiation was not found. Only extended cultures for osteoclastogenesis exponentially increased the frequency of osteoclast precursors. It is likely that C7 cell differentiation into committed osteoclast precursors is on 'autopilot' rather than requiring specific signals to drive this process.},
doi = {10.1016/J.BBRC.2012.10.052},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 428,
place = {United States},
year = {2012},
month = {11}
}