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Title: Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation

Abstract

Highlights: Black-Right-Pointing-Pointer Electrorotation offers a non-invasive tool for dielectric analysis of fish embryos. Black-Right-Pointing-Pointer The three-shell dielectric model matches the rotation spectra of medaka eggs. Black-Right-Pointing-Pointer The capacitance value suggests a double-membrane structure of yolk envelope. -- Abstract: The Japanese medaka fish, Oryzias latipes, has become a powerful vertebrate model organism in developmental biology and genetics. The present study explores the dielectric properties of medaka embryos during pre-hatching development by means of the electrorotation (ROT) technique. Due to their layered structure, medaka eggs exhibited up to three ROT peaks in the kHz-MHz frequency range. During development from blastula to early somite stage, ROT spectra varied only slightly. But as the embryo progressed to the late-somite stage, the ROT peaks underwent significant changes in frequency and amplitude. Using morphological data obtained by light and electron microscopy, we analyzed the ROT spectra with a three-shell dielectric model that accounted for the major embryonic compartments. The analysis yielded a very high value for the ionic conductivity of the egg shell (chorion), which was confirmed by independent osmotic experiments. A relatively low capacitance of the yolk envelope was consistent with its double-membrane structure revealed by transmission electron microscopy. Yolk-free dead eggs exhibited only onemore » co-field ROT peak, shifted markedly to lower frequencies with respect to the corresponding peak of live embryos. The dielectric data may be useful for monitoring the development and changes in fish embryos' viability/conditions in basic research and industrial aquaculture.« less

Authors:
 [1];  [2];  [3];  [2];  [4];  [5];  [6];  [2]
  1. Institute of Industrial Science, The University of Tokyo, Tokyo 153-8505 (Japan)
  2. Lehrstuhl fuer Biotechnologie und Biophysik, Biozentrum, Universitaet Wuerzburg, Wuerzburg (Germany)
  3. Physiologische Chemie, Biozentrum, Universitaet Wuerzburg, Wuerzburg (Germany)
  4. Abteilung fuer Elektronenmikroskopie, Biozentrum, Universitaet Wuerzburg, Wuerzburg (Germany)
  5. Lehrstuhl fuer Biotechnologie und Medizintechnik, Universitaet des Saarlandes, Saarbruecken (Germany)
  6. Lehrstuhl fuer Molekulare und Zellulaere Biotechnologie, Universitaet des Saarlandes, Saarbruecken (Germany)
Publication Date:
OSTI Identifier:
22210325
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 428; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 60 APPLIED LIFE SCIENCES; DIELECTRIC MATERIALS; EGGS; EMBRYOS; GENETICS; PERMITTIVITY; TRANSMISSION ELECTRON MICROSCOPY

Citation Formats

Shirakashi, Ryo, E-mail: aa21150@iis.u-tokyo.ac.jp, Mischke, Miriam, Fischer, Peter, Memmel, Simon, Krohne, Georg, Fuhr, Guenter R., Zimmermann, Heiko, and Sukhorukov, Vladimir L., E-mail: sukhorukov@biozentrum.uni-wuerzburg.de. Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.10.019.
Shirakashi, Ryo, E-mail: aa21150@iis.u-tokyo.ac.jp, Mischke, Miriam, Fischer, Peter, Memmel, Simon, Krohne, Georg, Fuhr, Guenter R., Zimmermann, Heiko, & Sukhorukov, Vladimir L., E-mail: sukhorukov@biozentrum.uni-wuerzburg.de. Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation. United States. doi:10.1016/J.BBRC.2012.10.019.
Shirakashi, Ryo, E-mail: aa21150@iis.u-tokyo.ac.jp, Mischke, Miriam, Fischer, Peter, Memmel, Simon, Krohne, Georg, Fuhr, Guenter R., Zimmermann, Heiko, and Sukhorukov, Vladimir L., E-mail: sukhorukov@biozentrum.uni-wuerzburg.de. Fri . "Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation". United States. doi:10.1016/J.BBRC.2012.10.019.
@article{osti_22210325,
title = {Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation},
author = {Shirakashi, Ryo, E-mail: aa21150@iis.u-tokyo.ac.jp and Mischke, Miriam and Fischer, Peter and Memmel, Simon and Krohne, Georg and Fuhr, Guenter R. and Zimmermann, Heiko and Sukhorukov, Vladimir L., E-mail: sukhorukov@biozentrum.uni-wuerzburg.de},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Electrorotation offers a non-invasive tool for dielectric analysis of fish embryos. Black-Right-Pointing-Pointer The three-shell dielectric model matches the rotation spectra of medaka eggs. Black-Right-Pointing-Pointer The capacitance value suggests a double-membrane structure of yolk envelope. -- Abstract: The Japanese medaka fish, Oryzias latipes, has become a powerful vertebrate model organism in developmental biology and genetics. The present study explores the dielectric properties of medaka embryos during pre-hatching development by means of the electrorotation (ROT) technique. Due to their layered structure, medaka eggs exhibited up to three ROT peaks in the kHz-MHz frequency range. During development from blastula to early somite stage, ROT spectra varied only slightly. But as the embryo progressed to the late-somite stage, the ROT peaks underwent significant changes in frequency and amplitude. Using morphological data obtained by light and electron microscopy, we analyzed the ROT spectra with a three-shell dielectric model that accounted for the major embryonic compartments. The analysis yielded a very high value for the ionic conductivity of the egg shell (chorion), which was confirmed by independent osmotic experiments. A relatively low capacitance of the yolk envelope was consistent with its double-membrane structure revealed by transmission electron microscopy. Yolk-free dead eggs exhibited only one co-field ROT peak, shifted markedly to lower frequencies with respect to the corresponding peak of live embryos. The dielectric data may be useful for monitoring the development and changes in fish embryos' viability/conditions in basic research and industrial aquaculture.},
doi = {10.1016/J.BBRC.2012.10.019},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 428,
place = {United States},
year = {2012},
month = {11}
}