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Title: Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds

Abstract

Highlights: Black-Right-Pointing-Pointer Neuron cancer stem cells (NCSCs) behave high multiply of growth on collagen scaffold. Black-Right-Pointing-Pointer Enhancement of NCSCs neurite outgrowth on porous collagen scaffold. Black-Right-Pointing-Pointer 3-D collagen culture of NCSCs shows an advance differentiation than 2-D culture. -- Abstract: Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 {mu}m porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells.more » This result could provide a new application for nerve tissue engineering and nerve regeneration.« less

Authors:
 [1];  [2];  [2];  [3];  [4];  [5];  [1];  [5]
  1. Department of Electrical Engineering, I-Shou University, Taiwan, ROC (China)
  2. (China)
  3. Department of Biomedical Engineering, I-Shou University, Taiwan, ROC (China)
  4. Centre for Eye Research Australia, University of Melbourne (Australia)
  5. Department of Biological Science and Technology, I-Shou University, Taiwan, ROC (China)
Publication Date:
OSTI Identifier:
22210321
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 428; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; COLLAGEN; FLUORESCENCE; MORPHOLOGICAL CHANGES; NEOPLASMS; NERVE CELLS; NERVE TISSUE; POROUS MATERIALS; REGENERATION; STEM CELLS

Citation Formats

Chen, Chih-Hao, Neurosurgery, Department of Surgery, Kaohsiung Veterans General Hospital, Taiwan, ROC, Department of Biomedical Engineering, I-Shou University, Taiwan, ROC, Kuo, Shyh Ming, Liu, Guei-Sheung, Chen, Wan-Nan U., Chuang, Chin-Wen, and Liu, Li-Feng, E-mail: liulf@isu.edu.tw. Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.10.007.
Chen, Chih-Hao, Neurosurgery, Department of Surgery, Kaohsiung Veterans General Hospital, Taiwan, ROC, Department of Biomedical Engineering, I-Shou University, Taiwan, ROC, Kuo, Shyh Ming, Liu, Guei-Sheung, Chen, Wan-Nan U., Chuang, Chin-Wen, & Liu, Li-Feng, E-mail: liulf@isu.edu.tw. Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds. United States. doi:10.1016/J.BBRC.2012.10.007.
Chen, Chih-Hao, Neurosurgery, Department of Surgery, Kaohsiung Veterans General Hospital, Taiwan, ROC, Department of Biomedical Engineering, I-Shou University, Taiwan, ROC, Kuo, Shyh Ming, Liu, Guei-Sheung, Chen, Wan-Nan U., Chuang, Chin-Wen, and Liu, Li-Feng, E-mail: liulf@isu.edu.tw. Fri . "Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds". United States. doi:10.1016/J.BBRC.2012.10.007.
@article{osti_22210321,
title = {Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds},
author = {Chen, Chih-Hao and Neurosurgery, Department of Surgery, Kaohsiung Veterans General Hospital, Taiwan, ROC and Department of Biomedical Engineering, I-Shou University, Taiwan, ROC and Kuo, Shyh Ming and Liu, Guei-Sheung and Chen, Wan-Nan U. and Chuang, Chin-Wen and Liu, Li-Feng, E-mail: liulf@isu.edu.tw},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Neuron cancer stem cells (NCSCs) behave high multiply of growth on collagen scaffold. Black-Right-Pointing-Pointer Enhancement of NCSCs neurite outgrowth on porous collagen scaffold. Black-Right-Pointing-Pointer 3-D collagen culture of NCSCs shows an advance differentiation than 2-D culture. -- Abstract: Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 {mu}m porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells. This result could provide a new application for nerve tissue engineering and nerve regeneration.},
doi = {10.1016/J.BBRC.2012.10.007},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 428,
place = {United States},
year = {2012},
month = {11}
}