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Title: End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes

Abstract

Highlights: Black-Right-Pointing-Pointer Multifunctional enzymes offer an interesting approach for biomass degradation. Black-Right-Pointing-Pointer Size and conformation of separate constructs play a role in the effectiveness of chimeras. Black-Right-Pointing-Pointer A connecting linker allows for maximal flexibility and increased thermostability. Black-Right-Pointing-Pointer Genes with functional similarities are the best choice for fusion candidates. -- Abstract: The reduction of fossil fuels, coupled with its increase in price, has made the search for alternative energy resources more plausible. One of the topics gaining fast interest is the utilization of lignocellulose, the main component of plants. Its primary constituents, cellulose and hemicellulose, can be degraded by a series of enzymes present in microorganisms, into simple sugars, later used for bioethanol production. Thermophilic bacteria have proven to be an interesting source of enzymes required for hydrolysis since they can withstand high and denaturing temperatures, which are usually required for processes involving biomass degradation. However, the cost associated with the whole enzymatic process is staggering. A solution for cost effective and highly active production is through the construction of multifunctional enzyme complexes harboring the function of more than one enzyme needed for the hydrolysis process. There are various strategies for the degradation of complex biomass ranging from the regulationmore » of the enzymes involved, to cellulosomes, and proteins harboring more than one enzymatic activity. In this review, the construction of multifunctional biomass degrading enzymes through end-to-end gene fusions, and its impact on production and activity by choosing the enzymes and linkers is assessed.« less

Authors:
 [1];  [1];  [1]
  1. Institute of Technical Microbiology, Hamburg University of Technology (TUHH), Kasernenstr. 12, D-21073 Hamburg (Germany)
Publication Date:
OSTI Identifier:
22210320
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 428; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BACTERIA; BIOETHANOL; BIOMASS; CELLULASE; CELLULOSE; GENES; HEMICELLULOSE; HYDROLYSIS; SACCHAROSE; XYLANASE

Citation Formats

Rizk, Mazen, E-mail: mazen.rizk@tuhh.de, Antranikian, Garabed, E-mail: antranikian@tuhh.de, and Elleuche, Skander, E-mail: skander.elleuche@tuhh.de. End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.09.142.
Rizk, Mazen, E-mail: mazen.rizk@tuhh.de, Antranikian, Garabed, E-mail: antranikian@tuhh.de, & Elleuche, Skander, E-mail: skander.elleuche@tuhh.de. End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes. United States. doi:10.1016/J.BBRC.2012.09.142.
Rizk, Mazen, E-mail: mazen.rizk@tuhh.de, Antranikian, Garabed, E-mail: antranikian@tuhh.de, and Elleuche, Skander, E-mail: skander.elleuche@tuhh.de. Fri . "End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes". United States. doi:10.1016/J.BBRC.2012.09.142.
@article{osti_22210320,
title = {End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes},
author = {Rizk, Mazen, E-mail: mazen.rizk@tuhh.de and Antranikian, Garabed, E-mail: antranikian@tuhh.de and Elleuche, Skander, E-mail: skander.elleuche@tuhh.de},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Multifunctional enzymes offer an interesting approach for biomass degradation. Black-Right-Pointing-Pointer Size and conformation of separate constructs play a role in the effectiveness of chimeras. Black-Right-Pointing-Pointer A connecting linker allows for maximal flexibility and increased thermostability. Black-Right-Pointing-Pointer Genes with functional similarities are the best choice for fusion candidates. -- Abstract: The reduction of fossil fuels, coupled with its increase in price, has made the search for alternative energy resources more plausible. One of the topics gaining fast interest is the utilization of lignocellulose, the main component of plants. Its primary constituents, cellulose and hemicellulose, can be degraded by a series of enzymes present in microorganisms, into simple sugars, later used for bioethanol production. Thermophilic bacteria have proven to be an interesting source of enzymes required for hydrolysis since they can withstand high and denaturing temperatures, which are usually required for processes involving biomass degradation. However, the cost associated with the whole enzymatic process is staggering. A solution for cost effective and highly active production is through the construction of multifunctional enzyme complexes harboring the function of more than one enzyme needed for the hydrolysis process. There are various strategies for the degradation of complex biomass ranging from the regulation of the enzymes involved, to cellulosomes, and proteins harboring more than one enzymatic activity. In this review, the construction of multifunctional biomass degrading enzymes through end-to-end gene fusions, and its impact on production and activity by choosing the enzymes and linkers is assessed.},
doi = {10.1016/J.BBRC.2012.09.142},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 428,
place = {United States},
year = {2012},
month = {11}
}