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Title: Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells

Abstract

Highlights: Black-Right-Pointing-Pointer Silica nanoparticles enhanced cross-presentation. Black-Right-Pointing-Pointer Silica nanoparticles induced endosomal release of exogenous antigens. Black-Right-Pointing-Pointer Silica nanoparticle-induced cross-presentation was mediated by scavenger receptors. Black-Right-Pointing-Pointer Surface-modification may enable the manufacture of safer silica nanoparticles. -- Abstract: Nanomaterials (NMs) exhibit unique physicochemical properties and innovative functions, and they are increasingly being used in a wide variety of fields. Ensuring the safety of NMs is now an urgent task. Recently, we reported that amorphous silica nanoparticles (nSPs), one of the most widely used NMs, enhance antigen-specific cellular immune responses and may therefore aggravate immune diseases. Thus, to ensure the design of safer nSPs, investigations into the effect of nSPs on antigen presentation in dendritic cells, which are central orchestrators of the adaptive immune response, are now needed. Here, we show that nSPs with diameters of 70 and 100 nm enhanced exogenous antigen entry into the cytosol from endosomes and induced cross-presentation, whereas submicron-sized silica particles (>100 nm) did not. Furthermore, we show that surface modification of nSPs suppressed cross-presentation. Although further studies are required to investigate whether surface-modified nSPs suppress immune-modulating effects in vivo, the current results indicate that appropriate regulation of the characteristics of nSPs, such as size and surface properties,more » will be critical for the design of safer nSPs.« less

Authors:
 [1];  [1]; ; ; ;  [1];  [2];  [3]; ;  [2];  [4];  [5];  [1];  [1];  [4];  [4]
  1. Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871 (Japan)
  2. Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, 7-6-8 Saitoasagi, Ibaraki, Osaka 567-0085 (Japan)
  3. Cancer Biology Research Center, Sanford Research/USD, 2301 E. 60th Street N, Sioux Falls, SD 57104 (United States)
  4. (Japan)
  5. Division of Foods, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501 (Japan)
Publication Date:
OSTI Identifier:
22210305
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 427; Journal Issue: 3; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; DENDRITES; IN VIVO; NANOSTRUCTURES; OVA; OVALBUMIN; RECEPTORS; SILICA; SURFACE PROPERTIES

Citation Formats

Hirai, Toshiro, Yoshioka, Yasuo, E-mail: yasuo@phs.osaka-u.ac.jp, Takahashi, Hideki, Ichihashi, Ko-ichi, Yoshida, Tokuyuki, Tochigi, Saeko, Nagano, Kazuya, Abe, Yasuhiro, Kamada, Haruhiko, Tsunoda, Shin-ichi, The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Nabeshi, Hiromi, Yoshikawa, Tomoaki, Tsutsumi, Yasuo, E-mail: ytsutsumi@phs.osaka-u.ac.jp, Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, 7-6-8 Saitoasagi, Ibaraki, Osaka 567-0085, and The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871. Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.09.095.
Hirai, Toshiro, Yoshioka, Yasuo, E-mail: yasuo@phs.osaka-u.ac.jp, Takahashi, Hideki, Ichihashi, Ko-ichi, Yoshida, Tokuyuki, Tochigi, Saeko, Nagano, Kazuya, Abe, Yasuhiro, Kamada, Haruhiko, Tsunoda, Shin-ichi, The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Nabeshi, Hiromi, Yoshikawa, Tomoaki, Tsutsumi, Yasuo, E-mail: ytsutsumi@phs.osaka-u.ac.jp, Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, 7-6-8 Saitoasagi, Ibaraki, Osaka 567-0085, & The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871. Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells. United States. doi:10.1016/J.BBRC.2012.09.095.
Hirai, Toshiro, Yoshioka, Yasuo, E-mail: yasuo@phs.osaka-u.ac.jp, Takahashi, Hideki, Ichihashi, Ko-ichi, Yoshida, Tokuyuki, Tochigi, Saeko, Nagano, Kazuya, Abe, Yasuhiro, Kamada, Haruhiko, Tsunoda, Shin-ichi, The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Nabeshi, Hiromi, Yoshikawa, Tomoaki, Tsutsumi, Yasuo, E-mail: ytsutsumi@phs.osaka-u.ac.jp, Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, 7-6-8 Saitoasagi, Ibaraki, Osaka 567-0085, and The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871. Fri . "Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells". United States. doi:10.1016/J.BBRC.2012.09.095.
@article{osti_22210305,
title = {Amorphous silica nanoparticles enhance cross-presentation in murine dendritic cells},
author = {Hirai, Toshiro and Yoshioka, Yasuo, E-mail: yasuo@phs.osaka-u.ac.jp and Takahashi, Hideki and Ichihashi, Ko-ichi and Yoshida, Tokuyuki and Tochigi, Saeko and Nagano, Kazuya and Abe, Yasuhiro and Kamada, Haruhiko and Tsunoda, Shin-ichi and The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871 and Nabeshi, Hiromi and Yoshikawa, Tomoaki and Tsutsumi, Yasuo, E-mail: ytsutsumi@phs.osaka-u.ac.jp and Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, 7-6-8 Saitoasagi, Ibaraki, Osaka 567-0085 and The Center for Advanced Medical Engineering and Informatics, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Silica nanoparticles enhanced cross-presentation. Black-Right-Pointing-Pointer Silica nanoparticles induced endosomal release of exogenous antigens. Black-Right-Pointing-Pointer Silica nanoparticle-induced cross-presentation was mediated by scavenger receptors. Black-Right-Pointing-Pointer Surface-modification may enable the manufacture of safer silica nanoparticles. -- Abstract: Nanomaterials (NMs) exhibit unique physicochemical properties and innovative functions, and they are increasingly being used in a wide variety of fields. Ensuring the safety of NMs is now an urgent task. Recently, we reported that amorphous silica nanoparticles (nSPs), one of the most widely used NMs, enhance antigen-specific cellular immune responses and may therefore aggravate immune diseases. Thus, to ensure the design of safer nSPs, investigations into the effect of nSPs on antigen presentation in dendritic cells, which are central orchestrators of the adaptive immune response, are now needed. Here, we show that nSPs with diameters of 70 and 100 nm enhanced exogenous antigen entry into the cytosol from endosomes and induced cross-presentation, whereas submicron-sized silica particles (>100 nm) did not. Furthermore, we show that surface modification of nSPs suppressed cross-presentation. Although further studies are required to investigate whether surface-modified nSPs suppress immune-modulating effects in vivo, the current results indicate that appropriate regulation of the characteristics of nSPs, such as size and surface properties, will be critical for the design of safer nSPs.},
doi = {10.1016/J.BBRC.2012.09.095},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 427,
place = {United States},
year = {2012},
month = {10}
}