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Title: BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation

Abstract

Highlights: Black-Right-Pointing-Pointer It has been thought that BDNF is not produced in the megakaryocytic lineage. Black-Right-Pointing-Pointer MEG-01 produces BDNF upon TPO stimulation and regulates its proliferation. Black-Right-Pointing-Pointer BDNF accelerates proliferation of MEG-01 in an autocrine manner. Black-Right-Pointing-Pointer BDNF may be an autocrine MEG-CSF, which regulates megakaryopoiesis. -- Abstract: While human platelets release endogenous brain-derived neurotrophic factor (BDNF) upon activation, a previous report on MEG-01, a megakaryocytic cell line, found no trace of BDNF production, and the pathophysiological function of platelet BDNF has remained elusive. In the present study, we demonstrate that MEG-01 produces BDNF in the presence of TPO and that this serves to potentiate cell proliferation. Our in vitro findings suggest that BDNF regulates MEG-01 proliferation in an autocrine manner, and we suggest that BDNF may be a physiological autocrine regulator of megakaryocyte progenitors.

Authors:
 [1];  [2]; ; ;  [1]; ; ;  [3];  [4];  [5]
  1. Graduate School of Health Sciences, Hokkaido University, Sapporo (Japan)
  2. (Japan)
  3. Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo (Japan)
  4. Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi (Japan)
  5. Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo (Japan)
Publication Date:
OSTI Identifier:
22210304
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 427; Journal Issue: 3; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BONE MARROW CELLS; BRAIN; CELL PROLIFERATION; COLONY FORMING UNITS; IN VITRO; STEM CELLS; STIMULATION

Citation Formats

Tamura, Shogo, Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Nagasawa, Ayumi, Masuda, Yuya, Tsunematsu, Tetsuya, Hayasaka, Koji, Matsuno, Kazuhiko, Shimizu, Chikara, Ozaki, Yukio, and Moriyama, Takanori, E-mail: moriyama@hs.hokuda.ac.jp. BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.09.093.
Tamura, Shogo, Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Nagasawa, Ayumi, Masuda, Yuya, Tsunematsu, Tetsuya, Hayasaka, Koji, Matsuno, Kazuhiko, Shimizu, Chikara, Ozaki, Yukio, & Moriyama, Takanori, E-mail: moriyama@hs.hokuda.ac.jp. BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation. United States. doi:10.1016/J.BBRC.2012.09.093.
Tamura, Shogo, Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Nagasawa, Ayumi, Masuda, Yuya, Tsunematsu, Tetsuya, Hayasaka, Koji, Matsuno, Kazuhiko, Shimizu, Chikara, Ozaki, Yukio, and Moriyama, Takanori, E-mail: moriyama@hs.hokuda.ac.jp. Fri . "BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation". United States. doi:10.1016/J.BBRC.2012.09.093.
@article{osti_22210304,
title = {BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation},
author = {Tamura, Shogo and Research Fellow of the Japan Society for the Promotion of Science, Tokyo and Nagasawa, Ayumi and Masuda, Yuya and Tsunematsu, Tetsuya and Hayasaka, Koji and Matsuno, Kazuhiko and Shimizu, Chikara and Ozaki, Yukio and Moriyama, Takanori, E-mail: moriyama@hs.hokuda.ac.jp},
abstractNote = {Highlights: Black-Right-Pointing-Pointer It has been thought that BDNF is not produced in the megakaryocytic lineage. Black-Right-Pointing-Pointer MEG-01 produces BDNF upon TPO stimulation and regulates its proliferation. Black-Right-Pointing-Pointer BDNF accelerates proliferation of MEG-01 in an autocrine manner. Black-Right-Pointing-Pointer BDNF may be an autocrine MEG-CSF, which regulates megakaryopoiesis. -- Abstract: While human platelets release endogenous brain-derived neurotrophic factor (BDNF) upon activation, a previous report on MEG-01, a megakaryocytic cell line, found no trace of BDNF production, and the pathophysiological function of platelet BDNF has remained elusive. In the present study, we demonstrate that MEG-01 produces BDNF in the presence of TPO and that this serves to potentiate cell proliferation. Our in vitro findings suggest that BDNF regulates MEG-01 proliferation in an autocrine manner, and we suggest that BDNF may be a physiological autocrine regulator of megakaryocyte progenitors.},
doi = {10.1016/J.BBRC.2012.09.093},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 427,
place = {United States},
year = {2012},
month = {10}
}