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Title: Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid

Abstract

Highlights: Black-Right-Pointing-Pointer Biocompatible room-temperature ionic liquid was applied on beating cardiomyocyte. Black-Right-Pointing-Pointer The lifetime of beating cardiomyocytes was depended on anion functional group. Black-Right-Pointing-Pointer A longer lifetime was recorded for no functional group on alkyl chain on their anion. Black-Right-Pointing-Pointer Amino group on alkyl chain and fluorine in anion induced fatal condition changes. Black-Right-Pointing-Pointer We reported liquid electrolyte interface to stimulate cardiomyocytes. -- Abstract: Room-temperature ionic liquids (RTILs) are drawing attention as a new class of nonaqueous solvents to replace organic and aqueous solvents for chemical processes in the liquid phase at room temperature. The RTILs are notable for their characteristics of nonvolatility, extremely low vapor pressure, electric conductivity, and incombustibility. These distinguished properties of RTILs have brought attention to them in applications with biological cells and tissue in vacuum environment for scanning electron microscopy, and in microfluidic devices for micro-total analysis system (micro-TAS). Habitable RTILs could increase capability of nonaqueous micro-TAS for living cells. Some RTILs seemed to have the capability to replace water in biological applications. However, these RTILs had been applied to just supplemental additives for biocompatible test, to fixed cells as a substitute for an aqueous solution, and to simple molecules. None of RTILs in whichmore » directly soaks a living cell culture. Therefore, we demonstrated the design of RTILs for a living cell culture and a liquid electrolyte to stimulate contracting cardiomyocytes using the RTILs. We assessed the effect of RTILs on the cardiomyocytes using the beating lifetime to compare the applicability of RTILs for biological applications. Frequent spontaneous contractions of cardiomyocytes were confirmed in amino acid anion RTILs [P{sub 8,8,8,8}][Leu] and [P{sub 8,8,8,8}][Ala], phosphoric acid derivatives [P{sub 8,8,8,8}][MeO(H)PO{sub 2}], and [P{sub 8,8,8,8}][C{sub 7}CO{sub 2}]. The anion type of RTILs had influence on applicable characteristics for the contracting cardiomyocyte. This result suggested the possibility for biocompatible design of hydrophobic group RTILs to achieve biological applications with living cells.« less

Authors:
 [1];  [2]; ;  [3];  [2];  [1]
  1. Department of Mechanical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 (Japan)
  2. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588 (Japan)
  3. Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588 (Japan)
Publication Date:
OSTI Identifier:
22210296
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 427; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES; AMINO ACIDS; ANIMAL TISSUES; AQUEOUS SOLUTIONS; CELL CULTURES; ELECTRIC CONDUCTIVITY; ELECTROLYTES; FLUORINE; MOLTEN SALTS; NONAQUEOUS SOLVENTS; PARTIAL PRESSURE; SCANNING ELECTRON MICROSCOPY; VAPOR PRESSURE

Citation Formats

Hoshino, Takayuki, Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Fujita, Kyoko, Higashi, Ayako, Sakiyama, Keiko, Ohno, Hiroyuki, Morishima, Keisuke, and Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588. Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.09.068.
Hoshino, Takayuki, Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Fujita, Kyoko, Higashi, Ayako, Sakiyama, Keiko, Ohno, Hiroyuki, Morishima, Keisuke, & Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588. Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid. United States. https://doi.org/10.1016/J.BBRC.2012.09.068
Hoshino, Takayuki, Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Fujita, Kyoko, Higashi, Ayako, Sakiyama, Keiko, Ohno, Hiroyuki, Morishima, Keisuke, and Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588. 2012. "Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid". United States. https://doi.org/10.1016/J.BBRC.2012.09.068.
@article{osti_22210296,
title = {Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid},
author = {Hoshino, Takayuki and Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588 and Fujita, Kyoko and Higashi, Ayako and Sakiyama, Keiko and Ohno, Hiroyuki and Morishima, Keisuke and Department of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Biocompatible room-temperature ionic liquid was applied on beating cardiomyocyte. Black-Right-Pointing-Pointer The lifetime of beating cardiomyocytes was depended on anion functional group. Black-Right-Pointing-Pointer A longer lifetime was recorded for no functional group on alkyl chain on their anion. Black-Right-Pointing-Pointer Amino group on alkyl chain and fluorine in anion induced fatal condition changes. Black-Right-Pointing-Pointer We reported liquid electrolyte interface to stimulate cardiomyocytes. -- Abstract: Room-temperature ionic liquids (RTILs) are drawing attention as a new class of nonaqueous solvents to replace organic and aqueous solvents for chemical processes in the liquid phase at room temperature. The RTILs are notable for their characteristics of nonvolatility, extremely low vapor pressure, electric conductivity, and incombustibility. These distinguished properties of RTILs have brought attention to them in applications with biological cells and tissue in vacuum environment for scanning electron microscopy, and in microfluidic devices for micro-total analysis system (micro-TAS). Habitable RTILs could increase capability of nonaqueous micro-TAS for living cells. Some RTILs seemed to have the capability to replace water in biological applications. However, these RTILs had been applied to just supplemental additives for biocompatible test, to fixed cells as a substitute for an aqueous solution, and to simple molecules. None of RTILs in which directly soaks a living cell culture. Therefore, we demonstrated the design of RTILs for a living cell culture and a liquid electrolyte to stimulate contracting cardiomyocytes using the RTILs. We assessed the effect of RTILs on the cardiomyocytes using the beating lifetime to compare the applicability of RTILs for biological applications. Frequent spontaneous contractions of cardiomyocytes were confirmed in amino acid anion RTILs [P{sub 8,8,8,8}][Leu] and [P{sub 8,8,8,8}][Ala], phosphoric acid derivatives [P{sub 8,8,8,8}][MeO(H)PO{sub 2}], and [P{sub 8,8,8,8}][C{sub 7}CO{sub 2}]. The anion type of RTILs had influence on applicable characteristics for the contracting cardiomyocyte. This result suggested the possibility for biocompatible design of hydrophobic group RTILs to achieve biological applications with living cells.},
doi = {10.1016/J.BBRC.2012.09.068},
url = {https://www.osti.gov/biblio/22210296}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 427,
place = {United States},
year = {2012},
month = {10}
}