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Title: Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells

Abstract

Highlights: Black-Right-Pointing-Pointer Co-culture of Sertoli cells (SCs) with human umbilical cord mesenchymal stem cells (UCMSCs). Black-Right-Pointing-Pointer Presence of SCs dramatically increased proliferation and migration of UCMSCs. Black-Right-Pointing-Pointer Presence of SCs stimulated expression of Mdm2, Akt, CDC2, Cyclin D, CXCR4, MAPKs. -- Abstract: Human umbilical cord mesenchymal stem cells (hUCMSCs) have been recently used in transplant therapy. The proliferation and migration of MSCs are the determinants of the efficiency of MSC transplant therapy. Sertoli cells are a kind of 'nurse' cells that support the development of sperm cells. Recent studies show that Sertoli cells promote proliferation of endothelial cells and neural stem cells in co-culture. We hypothesized that co-culture of UCMSCs with Sertoli cells may also promote proliferation and migration of UCMSCs. To examine this hypothesis, we isolated UCMSCs from human cords and Sertoli cells from mouse testes, and co-cultured them using a Transwell system. We found that UCMSCs exhibited strong proliferation ability and potential to differentiate to other cell lineages such as osteocytes and adipocytes. The presence of Sertoli cells in co-culture significantly enhanced the proliferation and migration potential of UCMSCs (P < 0.01). Moreover, these phenotypic changes were accompanied with upregulation of multiple genes involved in cell proliferation andmore » migration including phospho-Akt, Mdm2, phospho-CDC2, Cyclin D1, Cyclin D3 as well as CXCR4, phospho-p44 MAPK and phospho-p38 MAPK. These findings indicate that Sertoli cells boost UCMSC proliferation and migration potential.« less

Authors:
 [1]; ;  [2];  [1];  [3];  [4]
  1. Department of Anatomy, Sanquan College, Xinxiang Medical University, Henan 453003, People's Republic of China (China)
  2. Department of Histology and Embryology, Guiyang Medical University, Guizhou 550004, People's Republic of China (China)
  3. ICU Center, The Third Hospital of Xinxiang Medical University, Henan 453003, People's Republic of China (China)
  4. Stem Cell Center, Xinxiang Medical University, Henan 453003, People's Republic of China (China)
Publication Date:
OSTI Identifier:
22210276
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 427; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BONE CELLS; CELL PROLIFERATION; MICE; PROLIFERATION; SPERMATOZOA; STEM CELLS; TESTES; TRANSPLANTS

Citation Formats

Zhang, Fenxi, E-mail: fxzhang0824@gmail.com, Hong, Yan, Liang, Wenmei, Ren, Tongming, Jing, Suhua, and Lin, Juntang. Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.09.007.
Zhang, Fenxi, E-mail: fxzhang0824@gmail.com, Hong, Yan, Liang, Wenmei, Ren, Tongming, Jing, Suhua, & Lin, Juntang. Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells. United States. doi:10.1016/J.BBRC.2012.09.007.
Zhang, Fenxi, E-mail: fxzhang0824@gmail.com, Hong, Yan, Liang, Wenmei, Ren, Tongming, Jing, Suhua, and Lin, Juntang. Fri . "Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells". United States. doi:10.1016/J.BBRC.2012.09.007.
@article{osti_22210276,
title = {Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells},
author = {Zhang, Fenxi, E-mail: fxzhang0824@gmail.com and Hong, Yan and Liang, Wenmei and Ren, Tongming and Jing, Suhua and Lin, Juntang},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Co-culture of Sertoli cells (SCs) with human umbilical cord mesenchymal stem cells (UCMSCs). Black-Right-Pointing-Pointer Presence of SCs dramatically increased proliferation and migration of UCMSCs. Black-Right-Pointing-Pointer Presence of SCs stimulated expression of Mdm2, Akt, CDC2, Cyclin D, CXCR4, MAPKs. -- Abstract: Human umbilical cord mesenchymal stem cells (hUCMSCs) have been recently used in transplant therapy. The proliferation and migration of MSCs are the determinants of the efficiency of MSC transplant therapy. Sertoli cells are a kind of 'nurse' cells that support the development of sperm cells. Recent studies show that Sertoli cells promote proliferation of endothelial cells and neural stem cells in co-culture. We hypothesized that co-culture of UCMSCs with Sertoli cells may also promote proliferation and migration of UCMSCs. To examine this hypothesis, we isolated UCMSCs from human cords and Sertoli cells from mouse testes, and co-cultured them using a Transwell system. We found that UCMSCs exhibited strong proliferation ability and potential to differentiate to other cell lineages such as osteocytes and adipocytes. The presence of Sertoli cells in co-culture significantly enhanced the proliferation and migration potential of UCMSCs (P < 0.01). Moreover, these phenotypic changes were accompanied with upregulation of multiple genes involved in cell proliferation and migration including phospho-Akt, Mdm2, phospho-CDC2, Cyclin D1, Cyclin D3 as well as CXCR4, phospho-p44 MAPK and phospho-p38 MAPK. These findings indicate that Sertoli cells boost UCMSC proliferation and migration potential.},
doi = {10.1016/J.BBRC.2012.09.007},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 427,
place = {United States},
year = {Fri Oct 12 00:00:00 EDT 2012},
month = {Fri Oct 12 00:00:00 EDT 2012}
}