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Title: Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation

Abstract

Highlights: Black-Right-Pointing-Pointer Ultrasmall citrate-coated SPIONs with {gamma}Fe{sub 2}O{sub 3} and Fe{sub 3}O{sub 4} structure were prepared. Black-Right-Pointing-Pointer SPIONs uptaken by MCF-7 cells increase the ROS production for about 240%. Black-Right-Pointing-Pointer The SPION induced ROS production is due to released iron ions and catalytically active surfaces. Black-Right-Pointing-Pointer Released iron ions and SPION surfaces initiate the Fenton and Haber-Weiss reaction. Black-Right-Pointing-Pointer X-ray irradiation of internalized SPIONs leads to an increase of catalytically active surfaces. -- Abstract: Internalization of citrate-coated and uncoated superparamagnetic iron oxide nanoparticles by human breast cancer (MCF-7) cells was verified by transmission electron microscopy imaging. Cytotoxicity studies employing metabolic and trypan blue assays manifested their excellent biocompatibility. The production of reactive oxygen species in iron oxide nanoparticle loaded MCF-7 cells was explained to originate from both, the release of iron ions and their catalytically active surfaces. Both initiate the Fenton and Haber-Weiss reaction. Additional oxidative stress caused by X-ray irradiation of MCF-7 cells was attributed to the increase of catalytically active iron oxide nanoparticle surfaces.

Authors:
;  [1];  [2];  [3];  [1]
  1. Department of Chemistry and Pharmacy, Physical Chemistry I and ICMM, Friedrich-Alexander University of Erlangen-Nuremberg, Egerlandstr. 3, D-91058 Erlangen (Germany)
  2. Department of Radiation Oncology, Friedrich Alexander University Erlangen-Nuremberg, Universitaetsstrasse 27, D-91054 Erlangen (Germany)
  3. Department of Anatomy, Chair of Anatomy I, Friedrich Alexander University Erlangen-Nuremberg, Krankenhausstr. 9, D-91054 Erlangen (Germany)
Publication Date:
OSTI Identifier:
22210211
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 425; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; AUGER ELECTRON SPECTROSCOPY; CITRATES; FERRITES; IRON IONS; IRON OXIDES; IRRADIATION; MAMMARY GLANDS; NANOSTRUCTURES; NEOPLASMS; OXIDATION; OXYGEN; RADIOSENSITIVITY; RADIOSENSITIZERS; SPECTROMETERS; SUPERPARAMAGNETISM; TOXICITY; TRANSMISSION ELECTRON MICROSCOPY; TRYPAN BLUE; X RADIATION

Citation Formats

Klein, Stefanie, Sommer, Anja, Distel, Luitpold V.R., Neuhuber, Winfried, and Kryschi, Carola. Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.07.108.
Klein, Stefanie, Sommer, Anja, Distel, Luitpold V.R., Neuhuber, Winfried, & Kryschi, Carola. Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation. United States. https://doi.org/10.1016/J.BBRC.2012.07.108
Klein, Stefanie, Sommer, Anja, Distel, Luitpold V.R., Neuhuber, Winfried, and Kryschi, Carola. Fri . "Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation". United States. https://doi.org/10.1016/J.BBRC.2012.07.108.
@article{osti_22210211,
title = {Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation},
author = {Klein, Stefanie and Sommer, Anja and Distel, Luitpold V.R. and Neuhuber, Winfried and Kryschi, Carola},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Ultrasmall citrate-coated SPIONs with {gamma}Fe{sub 2}O{sub 3} and Fe{sub 3}O{sub 4} structure were prepared. Black-Right-Pointing-Pointer SPIONs uptaken by MCF-7 cells increase the ROS production for about 240%. Black-Right-Pointing-Pointer The SPION induced ROS production is due to released iron ions and catalytically active surfaces. Black-Right-Pointing-Pointer Released iron ions and SPION surfaces initiate the Fenton and Haber-Weiss reaction. Black-Right-Pointing-Pointer X-ray irradiation of internalized SPIONs leads to an increase of catalytically active surfaces. -- Abstract: Internalization of citrate-coated and uncoated superparamagnetic iron oxide nanoparticles by human breast cancer (MCF-7) cells was verified by transmission electron microscopy imaging. Cytotoxicity studies employing metabolic and trypan blue assays manifested their excellent biocompatibility. The production of reactive oxygen species in iron oxide nanoparticle loaded MCF-7 cells was explained to originate from both, the release of iron ions and their catalytically active surfaces. Both initiate the Fenton and Haber-Weiss reaction. Additional oxidative stress caused by X-ray irradiation of MCF-7 cells was attributed to the increase of catalytically active iron oxide nanoparticle surfaces.},
doi = {10.1016/J.BBRC.2012.07.108},
url = {https://www.osti.gov/biblio/22210211}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 425,
place = {United States},
year = {2012},
month = {8}
}