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Title: Peroxisome proliferator-activated receptor gamma overexpression suppresses proliferation of human colon cancer cells

Abstract

Highlights: Black-Right-Pointing-Pointer We examined the correlation between PPAR{gamma} expression and cell proliferation. Black-Right-Pointing-Pointer PPAR{gamma} overexpression reduces cell viability. Black-Right-Pointing-Pointer We show the synergistic effect of cell growth inhibition by a PPAR{gamma} agonist. -- Abstract: Peroxisome proliferator-activated receptor gamma (PPAR{gamma}) plays an important role in the differentiation of intestinal cells and tissues. Our previous reports indicate that PPAR{gamma} is expressed at considerable levels in human colon cancer cells. This suggests that PPAR{gamma} expression may be an important factor for cell growth regulation in colon cancer. In this study, we investigated PPAR{gamma} expression in 4 human colon cancer cell lines, HT-29, LOVO, DLD-1, and Caco-2. Real-time polymerase chain reaction (PCR) and Western blot analysis revealed that the relative levels of PPAR{gamma} mRNA and protein in these cells were in the order HT-29 > LOVO > Caco-2 > DLD-1. We also found that PPAR{gamma} overexpression promoted cell growth inhibition in PPAR{gamma} lower-expressing cell lines (Caco-2 and DLD-1), but not in higher-expressing cells (HT-29 and LOVO). We observed a correlation between the level of PPAR{gamma} expression and the cells' sensitivity for proliferation.

Authors:
 [1];  [2]
  1. Department of Integrative Physiology and Bio-System Control, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621 (Japan)
  2. Department of Orthopaedic Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621 (Japan)
Publication Date:
OSTI Identifier:
22210172
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 424; Journal Issue: 3; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; INHIBITION; LARGE INTESTINE; MESSENGER-RNA; NEOPLASMS; POLYMERASE CHAIN REACTION; RECEPTORS; SENSITIVITY

Citation Formats

Tsukahara, Tamotsu, E-mail: ttamotsu@shinshu-u.ac.jp, and Haniu, Hisao. Peroxisome proliferator-activated receptor gamma overexpression suppresses proliferation of human colon cancer cells. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.06.149.
Tsukahara, Tamotsu, E-mail: ttamotsu@shinshu-u.ac.jp, & Haniu, Hisao. Peroxisome proliferator-activated receptor gamma overexpression suppresses proliferation of human colon cancer cells. United States. doi:10.1016/J.BBRC.2012.06.149.
Tsukahara, Tamotsu, E-mail: ttamotsu@shinshu-u.ac.jp, and Haniu, Hisao. Fri . "Peroxisome proliferator-activated receptor gamma overexpression suppresses proliferation of human colon cancer cells". United States. doi:10.1016/J.BBRC.2012.06.149.
@article{osti_22210172,
title = {Peroxisome proliferator-activated receptor gamma overexpression suppresses proliferation of human colon cancer cells},
author = {Tsukahara, Tamotsu, E-mail: ttamotsu@shinshu-u.ac.jp and Haniu, Hisao},
abstractNote = {Highlights: Black-Right-Pointing-Pointer We examined the correlation between PPAR{gamma} expression and cell proliferation. Black-Right-Pointing-Pointer PPAR{gamma} overexpression reduces cell viability. Black-Right-Pointing-Pointer We show the synergistic effect of cell growth inhibition by a PPAR{gamma} agonist. -- Abstract: Peroxisome proliferator-activated receptor gamma (PPAR{gamma}) plays an important role in the differentiation of intestinal cells and tissues. Our previous reports indicate that PPAR{gamma} is expressed at considerable levels in human colon cancer cells. This suggests that PPAR{gamma} expression may be an important factor for cell growth regulation in colon cancer. In this study, we investigated PPAR{gamma} expression in 4 human colon cancer cell lines, HT-29, LOVO, DLD-1, and Caco-2. Real-time polymerase chain reaction (PCR) and Western blot analysis revealed that the relative levels of PPAR{gamma} mRNA and protein in these cells were in the order HT-29 > LOVO > Caco-2 > DLD-1. We also found that PPAR{gamma} overexpression promoted cell growth inhibition in PPAR{gamma} lower-expressing cell lines (Caco-2 and DLD-1), but not in higher-expressing cells (HT-29 and LOVO). We observed a correlation between the level of PPAR{gamma} expression and the cells' sensitivity for proliferation.},
doi = {10.1016/J.BBRC.2012.06.149},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 424,
place = {United States},
year = {Fri Aug 03 00:00:00 EDT 2012},
month = {Fri Aug 03 00:00:00 EDT 2012}
}