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Title: Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics

Abstract

Amphiregulin (AREG), a ligand for epidermal growth factor receptor, is required for mammary gland ductal morphogenesis and mediates estrogen actions in vivo, emerging as an essential growth factor during mammary gland growth and differentiation. The COMMA-D {beta}-geo (CD{beta}geo) mouse mammary cell line displays characteristics of normal mammary progenitor cells including the ability to regenerate a mammary gland when transplanted into the cleared fat pad of a juvenile mouse, nuclear label retention, and the capacity to form anchorage-independent mammospheres. We demonstrate that AREG is essential for formation of floating mammospheres by CD{beta}geo cells and that the mitogen activated protein kinase signaling pathway is involved in AREG-mediated mammosphere formation. Addition of exogenous AREG promotes mammosphere formation in cells where AREG expression is knocked down by siRNA and mammosphere formation by AREG{sup -/-} mammary epithelial cells. AREG knockdown inhibits mammosphere formation by duct-limited mammary progenitor cells but not lobule-limited mammary progenitor cells. These data demonstrate AREG mediates the function of a subset of mammary progenitor cells in vitro.

Authors:
 [1];  [2]; ;  [1]; ;  [3];  [1]
  1. Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 (United States)
  2. (United States)
  3. Ecole polytechnique federale de Lausanne (EPFL), ISREC-Swiss Institute for Experimental Research, NCCR Molecular Oncology, SV.832 Station 19 CH-1015, Lausanne (Switzerland)
Publication Date:
OSTI Identifier:
22209858
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 316; Journal Issue: 3; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BUDR; ESTROGENS; GROWTH FACTORS; IN VITRO; IN VIVO; MAMMARY GLANDS; MICE; MORPHOGENESIS; RECEPTORS; STEM CELLS

Citation Formats

Booth, Brian W., E-mail: brbooth@clemson.edu, Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC 29634, Boulanger, Corinne A., Anderson, Lisa H., Jimenez-Rojo, Lucia, Brisken, Cathrin, and Smith, Gilbert H., E-mail: gs4d@nih.gov. Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics. United States: N. p., 2010. Web. doi:10.1016/J.YEXCR.2009.11.006.
Booth, Brian W., E-mail: brbooth@clemson.edu, Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC 29634, Boulanger, Corinne A., Anderson, Lisa H., Jimenez-Rojo, Lucia, Brisken, Cathrin, & Smith, Gilbert H., E-mail: gs4d@nih.gov. Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics. United States. doi:10.1016/J.YEXCR.2009.11.006.
Booth, Brian W., E-mail: brbooth@clemson.edu, Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC 29634, Boulanger, Corinne A., Anderson, Lisa H., Jimenez-Rojo, Lucia, Brisken, Cathrin, and Smith, Gilbert H., E-mail: gs4d@nih.gov. Mon . "Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics". United States. doi:10.1016/J.YEXCR.2009.11.006.
@article{osti_22209858,
title = {Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics},
author = {Booth, Brian W., E-mail: brbooth@clemson.edu and Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC 29634 and Boulanger, Corinne A. and Anderson, Lisa H. and Jimenez-Rojo, Lucia and Brisken, Cathrin and Smith, Gilbert H., E-mail: gs4d@nih.gov},
abstractNote = {Amphiregulin (AREG), a ligand for epidermal growth factor receptor, is required for mammary gland ductal morphogenesis and mediates estrogen actions in vivo, emerging as an essential growth factor during mammary gland growth and differentiation. The COMMA-D {beta}-geo (CD{beta}geo) mouse mammary cell line displays characteristics of normal mammary progenitor cells including the ability to regenerate a mammary gland when transplanted into the cleared fat pad of a juvenile mouse, nuclear label retention, and the capacity to form anchorage-independent mammospheres. We demonstrate that AREG is essential for formation of floating mammospheres by CD{beta}geo cells and that the mitogen activated protein kinase signaling pathway is involved in AREG-mediated mammosphere formation. Addition of exogenous AREG promotes mammosphere formation in cells where AREG expression is knocked down by siRNA and mammosphere formation by AREG{sup -/-} mammary epithelial cells. AREG knockdown inhibits mammosphere formation by duct-limited mammary progenitor cells but not lobule-limited mammary progenitor cells. These data demonstrate AREG mediates the function of a subset of mammary progenitor cells in vitro.},
doi = {10.1016/J.YEXCR.2009.11.006},
journal = {Experimental Cell Research},
number = 3,
volume = 316,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 2010},
month = {Mon Feb 01 00:00:00 EST 2010}
}