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Title: MicroRNA-27a promotes myoblast proliferation by targeting myostatin

Abstract

Highlights: Black-Right-Pointing-Pointer We identified a myogenic role for miR-27a and a new target, myostatin. Black-Right-Pointing-Pointer The miR-27a was confirmed to target myostatin 3 Prime UTR. Black-Right-Pointing-Pointer miR-27a is upregulated and myostatin is downregulated during myoblast proliferation. Black-Right-Pointing-Pointer miR-27a promotes myoblast proliferation by reducing the expression of myostatin. -- Abstract: MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. However, the role of miRNAs in myoblast proliferation remains poorly understood. Here we found that the expression of miR-27a was increased during proliferation of C2C12 myoblasts. Moreover, overexpression of miR-27a in C2C12 cells promoted myoblast proliferation by reducing the expression of myostatin, a critical inhibitor of skeletal myogenesis. In addition, the miR-27a was confirmed to target myostatin 3 Prime UTR by a luciferase reporter analysis. Together, these results suggest that miR-27a promotes myoblast proliferation through targeting myostatin.

Authors:
; ; ;  [1];  [1]
  1. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014 (China)
Publication Date:
OSTI Identifier:
22207914
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 423; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; GENE REGULATION; LUCIFERASE; MYOBLASTS; RNA

Citation Formats

Huang, Zhiqing, Chen, Xiaoling, Yu, Bing, He, Jun, and Chen, Daiwen, E-mail: dwchen@sicau.edu.cn. MicroRNA-27a promotes myoblast proliferation by targeting myostatin. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.05.106.
Huang, Zhiqing, Chen, Xiaoling, Yu, Bing, He, Jun, & Chen, Daiwen, E-mail: dwchen@sicau.edu.cn. MicroRNA-27a promotes myoblast proliferation by targeting myostatin. United States. doi:10.1016/J.BBRC.2012.05.106.
Huang, Zhiqing, Chen, Xiaoling, Yu, Bing, He, Jun, and Chen, Daiwen, E-mail: dwchen@sicau.edu.cn. Fri . "MicroRNA-27a promotes myoblast proliferation by targeting myostatin". United States. doi:10.1016/J.BBRC.2012.05.106.
@article{osti_22207914,
title = {MicroRNA-27a promotes myoblast proliferation by targeting myostatin},
author = {Huang, Zhiqing and Chen, Xiaoling and Yu, Bing and He, Jun and Chen, Daiwen, E-mail: dwchen@sicau.edu.cn},
abstractNote = {Highlights: Black-Right-Pointing-Pointer We identified a myogenic role for miR-27a and a new target, myostatin. Black-Right-Pointing-Pointer The miR-27a was confirmed to target myostatin 3 Prime UTR. Black-Right-Pointing-Pointer miR-27a is upregulated and myostatin is downregulated during myoblast proliferation. Black-Right-Pointing-Pointer miR-27a promotes myoblast proliferation by reducing the expression of myostatin. -- Abstract: MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. However, the role of miRNAs in myoblast proliferation remains poorly understood. Here we found that the expression of miR-27a was increased during proliferation of C2C12 myoblasts. Moreover, overexpression of miR-27a in C2C12 cells promoted myoblast proliferation by reducing the expression of myostatin, a critical inhibitor of skeletal myogenesis. In addition, the miR-27a was confirmed to target myostatin 3 Prime UTR by a luciferase reporter analysis. Together, these results suggest that miR-27a promotes myoblast proliferation through targeting myostatin.},
doi = {10.1016/J.BBRC.2012.05.106},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 423,
place = {United States},
year = {2012},
month = {6}
}