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Title: TRIM45 negatively regulates NF-{kappa}B-mediated transcription and suppresses cell proliferation

Abstract

Highlights: Black-Right-Pointing-Pointer NF-{kappa}B plays an important role in cell survival and carcinogenesis. Black-Right-Pointing-Pointer TRIM45 negatively regulates TNF{alpha}-induced NF-{kappa}B-mediated transcription. Black-Right-Pointing-Pointer TRIM45 overexpression suppresses cell growth. Black-Right-Pointing-Pointer TRIM45 acts as a repressor for the NF-{kappa}B signal and regulates cell growth. -- Abstract: The NF-{kappa}B signaling pathway plays an important role in cell survival, immunity, inflammation, carcinogenesis, and organogenesis. Activation of NF-{kappa}B is regulated by several posttranslational modifications including phosphorylation, neddylation and ubiquitination. The NF-{kappa}B signaling pathway is activated by two distinct signaling mechanisms and is strictly modulated by the ubiquitin-proteasome system. It has been reported that overexpression of TRIM45, one of the TRIM family ubiquitin ligases, suppresses transcriptional activities of Elk-1 and AP-1, which are targets of the MAPK signaling pathway. In this study, we showed that TRIM45 also negatively regulates TNF{alpha}-induced NF-{kappa}B-mediated transcription by a luciferase reporter assay and that TRIM45 lacking a RING domain also has an activity to inhibit the NF-{kappa}B signal. Moreover, we found that TRIM45 overexpression suppresses cell growth. These findings suggest that TRIM45 acts as a repressor for the NF-{kappa}B signal and regulates cell growth.

Authors:
 [1];  [1];  [2];  [1];  [3];  [1]
  1. Department of Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638 (Japan)
  2. (Japan)
  3. Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638 (Japan)
Publication Date:
OSTI Identifier:
22207905
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 423; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CARCINOGENESIS; CELL PROLIFERATION; IMMUNITY; INFLAMMATION; LIGASES; LUCIFERASE; PHOSPHORYLATION; TRANSCRIPTION

Citation Formats

Shibata, Mio, Sato, Tomonobu, Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Nukiwa, Ryota, Ariga, Tadashi, and Hatakeyama, Shigetsugu, E-mail: hatas@med.hokudai.ac.jp. TRIM45 negatively regulates NF-{kappa}B-mediated transcription and suppresses cell proliferation. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.05.090.
Shibata, Mio, Sato, Tomonobu, Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Nukiwa, Ryota, Ariga, Tadashi, & Hatakeyama, Shigetsugu, E-mail: hatas@med.hokudai.ac.jp. TRIM45 negatively regulates NF-{kappa}B-mediated transcription and suppresses cell proliferation. United States. doi:10.1016/J.BBRC.2012.05.090.
Shibata, Mio, Sato, Tomonobu, Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Nukiwa, Ryota, Ariga, Tadashi, and Hatakeyama, Shigetsugu, E-mail: hatas@med.hokudai.ac.jp. Fri . "TRIM45 negatively regulates NF-{kappa}B-mediated transcription and suppresses cell proliferation". United States. doi:10.1016/J.BBRC.2012.05.090.
@article{osti_22207905,
title = {TRIM45 negatively regulates NF-{kappa}B-mediated transcription and suppresses cell proliferation},
author = {Shibata, Mio and Sato, Tomonobu and Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638 and Nukiwa, Ryota and Ariga, Tadashi and Hatakeyama, Shigetsugu, E-mail: hatas@med.hokudai.ac.jp},
abstractNote = {Highlights: Black-Right-Pointing-Pointer NF-{kappa}B plays an important role in cell survival and carcinogenesis. Black-Right-Pointing-Pointer TRIM45 negatively regulates TNF{alpha}-induced NF-{kappa}B-mediated transcription. Black-Right-Pointing-Pointer TRIM45 overexpression suppresses cell growth. Black-Right-Pointing-Pointer TRIM45 acts as a repressor for the NF-{kappa}B signal and regulates cell growth. -- Abstract: The NF-{kappa}B signaling pathway plays an important role in cell survival, immunity, inflammation, carcinogenesis, and organogenesis. Activation of NF-{kappa}B is regulated by several posttranslational modifications including phosphorylation, neddylation and ubiquitination. The NF-{kappa}B signaling pathway is activated by two distinct signaling mechanisms and is strictly modulated by the ubiquitin-proteasome system. It has been reported that overexpression of TRIM45, one of the TRIM family ubiquitin ligases, suppresses transcriptional activities of Elk-1 and AP-1, which are targets of the MAPK signaling pathway. In this study, we showed that TRIM45 also negatively regulates TNF{alpha}-induced NF-{kappa}B-mediated transcription by a luciferase reporter assay and that TRIM45 lacking a RING domain also has an activity to inhibit the NF-{kappa}B signal. Moreover, we found that TRIM45 overexpression suppresses cell growth. These findings suggest that TRIM45 acts as a repressor for the NF-{kappa}B signal and regulates cell growth.},
doi = {10.1016/J.BBRC.2012.05.090},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 423,
place = {United States},
year = {2012},
month = {6}
}